Article
Immunology
F. Morales-Leon, A. Opazo-Capurro, C. Caro, N. Lincopan, A. Cardenas-Arias, F. Esposito, V. Illesca, M. L. Rioseco, M. Dominguez-Yevenes, C. A. Lima, H. Bello-Toledo, Gerardo Gonzalez-Rocha
Summary: The convergence of virulence and multidrug-resistant phenotype is reported for the first time in Chile in Klebsiella pneumoniae complex isolates, indicating a clinical problem that requires special attention and continuous surveillance in South America. The detected hypervirulent isolates displayed resistance to broad-spectrum cephalosporins and carried multiple antibiotic-resistance genes, leading to a multidrug-resistant phenotype. In vivo virulence testing confirmed higher virulence of the isolates compared to the control strain.
Article
Microbiology
Hidenori Yamashiro, Yu Kasamatsu, Naomi Anan, Miki Takemura, Yoshinori Yamano
Summary: This study compared the efficacy of flomoxef with other beta-lactam antibiotics against extended-spectrum beta-lactamases (ESBL)-producing bacteria of clinical relevance. The results demonstrated that flomoxef showed good bactericidal effects and effectively inhibited the growth of ESBL-producing strains, even those with a high minimum inhibitory concentration (MIC) value. Therefore, flomoxef may be a promising alternative in the context of carbapenems sparing stewardship.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Satomi Takei, Yu Jie Lu, Mari Tohya, Shin Watanabe, Shigeki Misawa, Yoko Tabe, Takashi Miida, San Mya, Htay Htay Tin, Tatsuya Tada, Teruo Kirikae
Summary: This study demonstrates the spread of carbapenem-resistant K. pneumoniae strains in medical settings in Myanmar. Specifically, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in high-risk clones of K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Analytical
Manal Suleiman, George Abu-Aqil, Uraib Sharaha, Klaris Riesenberg, Orli Sagi, Itshak Lapidot, Mahmoud Huleihel, Ahmad Salman
Summary: Rapid detection of ESBL+ bacteria is crucial for efficient treatment of bacterial infections. Results from the study demonstrate the possibility of accurately identifying ESBL+ Klebsiella pneumoniae within about 20 minutes, with approximately 89% accuracy, sensitivity, and specificity.
Article
Infectious Diseases
Soo Tein Ngoi, Cindy Shuan Ju Teh, Chun Wie Chong, Kartini Abdul Jabar, Shiang Chiet Tan, Lean Huat Yu, Kin Chong Leong, Loong Hua Tee, Sazaly AbuBakar
Summary: The prevalence of ESBL-producing Enterobacteriaceae has impacted the efficacy of beta-lactam antibiotics in treating UTIs. This study showed high rates of resistance to various beta-lactam antibiotics in ESBL-producing E. coli and K. pneumoniae isolated from UTIs, with carbapenems remaining the most effective treatment option. Flomoxef may serve as a potential alternative to carbapenems for treating UTIs caused by ESBL-producers in Malaysia.
Article
Immunology
Lisandra Aguilar-Bultet, Claudia Bagutti, Adrian Egli, Monica Alt, Laura Maurer Pekerman, Ruth Schindler, Reto Furger, Lucas Eichenberger, Tim Roloff, Ingrid Steffen, Philipp Huebner, Tanja Stadler, Sarah Tschudin-Sutter
Summary: This report describes a cluster of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sequence type 101, derived from 1 poultry and 2 clinical samples collected within the setting of a prospective study designed to determine the diversity and migration of ESBL-producing Enterobacterales between humans, foodstuffs, and wastewater.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Health Care Sciences & Services
Narcisa Muresu, Giovanna Deiana, Marco Dettori, Alessandra Palmieri, Maria Dolores Masia, Andrea Cossu, Cristina D'Avino, Illari Sechi, Arcadia Del Rio, Andrea Piana, Paolo Castiglia
Summary: The spread of multi-drug resistant organisms (MDROs) is increasing globally. This report presents two independent cases of patients from Ukraine infected with Carbapenemase-producing Klebsiella pneumoniae, and the infection prevention control (IPC) strategies implemented to prevent further contamination. These cases highlight the importance of early recognition of multidrug-resistant bacteria, effective inter-hospital communication, antimicrobial stewardship protocols, and adequate IPC policies. Improvement in screening procedures is necessary to prevent potential nosocomial outbreaks and endemization.
Article
Infectious Diseases
Elena Sendra, Inmaculada Lopez Montesinos, Alicia Rodriguez-Alarcon, Juan Du, Ana Siverio-Pares, Mar Arenas-Miras, Esperanza Canas-Ruano, Nuria Prim, Xavier Duran-Jorda, Fabiola Blasco-Hernando, Enric Garcia-Alzorriz, Francesc Cots, Olivia Ferrandez, Silvia Gomez-Zorrilla
Summary: This study compared the clinical characteristics, outcomes, and economic differences between complicated urinary tract infections caused by extensively drug-resistant Pseudomonas aeruginosa and extended-spectrum beta-lactamase-producing Klebsiella pneumoniae. The results showed that patients with XDR P. aeruginosa cUTI more frequently received inappropriate empirical therapy, while ESBL-K. pneumoniae cUTI was associated with worse eradication rates, higher recurrence, and higher infection-related readmission. XDR P. aeruginosa cUTI was associated with worse early clinical cures and more antibiotic side effects, but no differences in mortality or hospitalization costs were observed.
Article
Biochemistry & Molecular Biology
Forough Babazadeh, Roghayeh Teimourpour, Mohsen Arzanlou, Hadi Peeridogaheh, Mehdi Yousefipour, Jafar MohammadShahi
Summary: The objective of this study was to evaluate the phenotypic and molecular characterization of ESBL/AmpC- and carbapenemase-producing K. pneumoniae isolates in Iran. The results showed that K. pneumoniae isolates had high resistance rates to cefazolin and cefotaxime, while meropenem and amikacin exhibited high sensitivity rates. The most frequent carbapenemase-encoding genes were bla(OXA-48) and bla(IMP), while bla(KPC) and bla(GTM) were not detected. Among the ESBL and AmpC beta-lactamase-encoding genes, bla(TEM) had the highest frequency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Multidisciplinary Sciences
Lucie Kraftova, Marc Finianos, Vendula Studentova, Katerina Chudejova, Vladislav Jakubu, Helena Zemlickova, Costas C. Papagiannitsis, Ibrahim Bitar, Jaroslav Hrabak
Summary: The ongoing spread of KPC-producing strains in Czech hospitals is described in this study, with different types of bla(KPC)-carrying plasmids spreading among various Enterobacterales species. Local dissemination of IncR and IncN plasmids was observed in some districts. The evolution of bla(KPC)-carrying plasmids through genetic rearrangements facilitates their preservation and further dissemination.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Daniel Martak, Julia Guther, Tess D. Verschuuren, Benoit Valot, Nadine Conzelmann, Stefanie Bunk, M. Eugenia Riccio, Elena Salamanca, Alexandre Meunier, Charles P. Henriot, Caroline Pressacco Brossier, Xavier Bertrand, Ben S. Cooper, Stephan Harbarth, Evelina Tacconelli, Ad C. Fluit, Jesus Rodriguez-Bano, Jan A. J. W. Kluytmans, Silke Peter, Didier Hocquet
Summary: This study in five European cities found that food items were not the main source of ESBL-Ec and ESBL-Kp, as the strains from contaminated environments were genetically distinct from those in food. The findings suggest that human-to-human transmission is possibly the most frequent route of ESBL-Ec and ESBL-Kp transmission in high-income countries.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Immunology
Eduardo Rodriguez-Noriega, Elvira Garza-Gonzalez, Paola Bocanegra-Ibarias, Beatriz Alejandra Paz-Velarde, Sergio Esparza-Ahumada, Esteban Gonzalez-Diaz, Hector R. Perez-Gomez, Rodrigo Escobedo-Sanchez, Gerardo Leon-Garnica, Rayo Morfin-Otero
Summary: This study identified the previous use of antibiotics, the use of a urinary catheter, the use of a central venous catheter, the use of mechanical ventilation, and ICU stay as predictors and independent risk factors for infection with NDM-1 K. pneumoniae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Lina Liu, Yu Feng, Li Wei, Yuling Xiao, Zhiyong Zong
Summary: A carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical strain carrying a novel bla(OXA-926) gene and belonging to the rare ST29 type was isolated from patient blood. The strain harbored a 94.9-kb self-transmissible IncFII plasmid with bla(KPC-2). Phylogenomic analysis of the ST29 complex identified 157 genomes. This complex is commonly linked to extended-spectrum beta-lactamase-encoding genes, particularly bla(CTX-M-15), but rarely carries carbapenemase genes. The novel plasmid-borne beta-lactamase bla(OXA-926) confers reduced susceptibility to specific beta-lactams and does not fully respond to avibactam, suggesting its potential origin from a species related to Variovorax guangxiensis >10 years ago.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Teresita d. J. Bello Gonzalez, Arie Kant, Quillan Dijkstra, Francesca Marcato, Kees van Reenen, Kees T. Veldman, Michael S. M. Brouwer
Summary: This study aimed to characterize the changes in fecal carriage of Extended-Spectrum beta-Lactamase (ESBL) producing Enterobacterales (ESBL-PE) over time in Dutch veal calves. The study found a decrease in fecal carriage of cefotaxime-resistant Escherichia coli isolates and an increase of cefotaxime-resistant Klebsiella pneumoniae isolates during the rearing period. The ESBL genes identified differed between the two bacteria. The study also discovered clonal spread of ESBL-K. pneumoniae in some animals.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
I Nakamura, T. Yamaguchi, Y. Miura, H. Watanabe
Summary: This study investigated the transmission of ESBL-producing Klebsiella pneumoniae strains from sinks and drains in a general surgical hospital ward. Through whole-genome analysis, it was found that the isolates were indistinguishable genetically, confirming this mode of transmission.
JOURNAL OF HOSPITAL INFECTION
(2021)