Article
Immunology
Gertrudis Horna, Joaquim Ruiz
Summary: Type 3 secretion systems (T3SSs) are crucial for bacterial pathogenesis, with impairing T3SS potentially serving as an alternative approach in combating bacterial infections. Various strategies, such as T3SS inhibition, vaccination, and antibody development, have been proposed to address this issue.
MICROBIAL PATHOGENESIS
(2021)
Article
Chemistry, Medicinal
Jun Liu, Qiu-Xian Chen, Wen-Fu Wu, Dong Wang, Si -Yu Zhao, Jia-Hao Li, Yi-Qun Chang, Shao-Gao Zeng, Jia-Yi Hu, Yu-Jie Li, Jia-Xin Du, Shu-Meng Jiao, Hai-Chuan Xiao, Qiang Zhang, Jun Xu, Jian-Fu Zhao, Hai -Bo Zhou, Yong-Heng Wang, Jian Zou, Ping-Hua Sun
Summary: A new anti-infective drug strategy has been discovered to attenuate virulence and modulate inflammation caused by drug-resistant Pseudomonas aeruginosa infections. Compound 5f inhibits biofilm formation, macrophage migration, and inflammatory response induced by P. aeruginosa, showing potential as a novel candidate against drug-resistant infections.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2024)
Review
Infectious Diseases
Virgyl Camberlein, Gwenaelle Jezequel, Joerg Haupenthal, Anna K. H. Hirsch
Summary: LasB, a zinc metalloprotease and a crucial virulence factor of Pseudomonas aeruginosa, has become a key target in the development of novel antivirulence agents. This review provides an overview of the structure of its active site and a summary of the disclosed P. aeruginosa LasB inhibitors, with a specific focus on their binding modes and strategies for targeting LasB by small molecules.
Article
Chemistry, Multidisciplinary
Cansu Kaya, Isabell Walter, Samir Yahiaoui, Asfandyar Sikandar, Alaa Alhayek, Jelena Konstantinovic, Andreas M. Kany, Jorg Haupenthal, Jesko Koehnke, Rolf W. Hartmann, Anna K. H. Hirsch
Summary: Extracellular virulence factors, including the LasB enzyme secreted by the Gram-negative pathogen Pseudomonas aeruginosa, have become important targets in the current antimicrobial resistance crisis. In this study, a sub-micromolar, non-peptidic inhibitor of LasB was discovered through structural data analysis and optimization, showing improved in vivo efficacy in Galleria mellonella larvae. This class of compounds shows promise as potential drug candidates for combating infections.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Multidisciplinary Sciences
Li Li, Zuxian Xu, Ruipin Cao, Jiaxin Li, Chang-Jer Wu, Yinglu Wang, Hu Zhu
Summary: We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of cyclic dipeptides against Pseudomonas aeruginosa PAO1. The presence of hydroxyl groups improved the inhibition of virulence factors and cytotoxicity, but reduced the ability to inhibit biofilm formation. These cyclic dipeptides interacted with the QS-related protein LasR, and the introduction of hydroxyl groups enhanced their self-assembly ability.
Review
Immunology
Pamella Constantino-Teles, Albane Jouault, Lhousseine Touqui, Alessandra Mattos Saliba
Summary: Pseudomonas aeruginosa, an opportunistic pathogen, causes respiratory infections by utilizing various virulence factors, such as bacterial lipids and enzymes, and by interacting with host lipids. These mechanisms play important roles in the establishment and persistence of P. aeruginosa in the lungs, as well as in modulating host immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Tomasz Bogiel, Malgorzata Prazynska, Joanna Kwiecinska-Pirog, Agnieszka Mikucka, Eugenia Gospodarek-Komkowska
Summary: The study compared the frequency of selected enzymatic virulence factor genes among non-duplicated carbapenem-resistant P. aeruginosa isolates, finding a higher prevalence of alkaline protease gene and a 37.4% detection rate for neuraminidase-1 gene. Significant differences in the prevalence of lasB and nan1 were observed between MBL-positive and MBL-negative strains.
Article
Biochemistry & Molecular Biology
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
Summary: This study assessed the susceptibility and virulence factor gene frequency of clinical P. aeruginosa strains causing bloodstream infections. The results showed that these strains were resistant to multiple antibiotics and had diverse compositions of virulence factor genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Microbiology
Zhidong Zhang, Yang Sun, Yuanyang Yi, Xiaoyu Bai, Liying Zhu, Jing Zhu, Meiying Gu, Yanlei Zhu, Ling Jiang
Summary: A strain of Streptomyces with quorum-sensing inhibitory activity was isolated from the Kumutag Desert in Xinjiang, and it effectively inhibited biofilm formation and virulence factor production in bacteria such as Pseudomonas aeruginosa.
Article
Microbiology
Gertrudis Horna, Joaquim Ruiz
Summary: Pseudomonas aeruginosa is an opportunistic pathogen that mainly affects severe patients in ICUs, characterized by high levels of antibiotic resistance and a long battery of virulence factors. Among these factors, T3SS plays a key role in injecting effectors into host cells and influencing immune responses to increase bacterial survival rates. Despite extensive research on T3SS, there are still questions and further points to be clarified or established. Efforts are being made to develop T3SS-targeting molecules to improve patient outcomes.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Youngsun Shin, Xi-Hui Li, Cheol Seung Lee, Joon-Hee Lee
Summary: Three major proteases in Pseudomonas aeruginosa, including LasB, PIV, and LasA, have important roles in infections and pathogeneses. LasB(pp), the propeptide of LasB, is not effectively inhibited due to its degradation by LasB. However, C-terminal modification of LasB(pp) can enhance its stability and resistance against LasB degradation.
JOURNAL OF MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tung Truong Thanh, Huy Luong Xuan, Thang Nguyen Quoc
Summary: Quorum sensing in bacteria is critical for cell-cell communication, and the discovery of novel compounds inhibiting this process is an emerging field. Evaluation of synthesized compounds revealed three potential quorum sensing inhibitors, with two showing promising activities in the LasB system. Further research may utilize these compounds as templates for drug development.
Review
Pharmacology & Pharmacy
Martin Everett, David Davies
Summary: The Pseudomonas aeruginosa LasB elastase is a key protease in the pathogen's secretome, involved in host protein degradation and immune response disruption. Developing specific LasB inhibitors could be a promising approach for the treatment of P. aeruginosa infections.
DRUG DISCOVERY TODAY
(2021)
Article
Physics, Applied
Caitlin Heslin, Daniela Boehm, Brendan F. Gilmore, Julianne Megaw, Paula Bourke
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2020)
Review
Biotechnology & Applied Microbiology
Stephen A. Kelly, Stefan Mix, Thomas S. Moody, Brendan F. Gilmore
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Physics, Applied
Nid'a H. Alshraiedeh, Stephen A. Kelly, Thomas P. Thompson, Padrig B. Flynn, Michael M. Tunney, Brendan F. Gilmore
PLASMA PROCESSES AND POLYMERS
(2020)
Article
Food Science & Technology
Helina Marshall, Julie P. Meneely, Brian Quinn, Yueju Zhao, Paula Bourke, Brendan F. Gilmore, Guangtao Zhang, Christopher T. Elliott
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2020)
Article
Physics, Multidisciplinary
Caitlin Heslin, Daniela Boehm, Brendan F. Gilmore, Julianne Megaw, Theresa A. Freeman, Noreen J. Hickok, P. J. Cullen, Paula Bourke
Summary: The potential applications of cold plasma in medicine are diverse, but a better understanding of its effects on complex biological pathways is still needed. Research indicates that cold plasma-treated biomolecules can impact cell behavior and in vivo functionality, with the effects depending on the type of molecule and treatment method.
FRONTIERS IN PHYSICS
(2021)
Article
Engineering, Manufacturing
Essyrose Mathew, Brendan F. Gilmore, Eneko Larraneta, Dimitrios A. Lamprou
Summary: The uncertainty of the COVID-19 pandemic has caused shortages in medical consumables and personal protective equipment, with 3D printing playing a crucial role in providing essential items. Antimicrobial materials show potential in manufacturing 3D printed essential products for added protection against pandemics.
3D PRINTING AND ADDITIVE MANUFACTURING
(2021)
Article
Environmental Sciences
Martin McLaughlin, Manuela A. Gilea, Martyn J. Earle, Kenneth R. Seddon, Brendan F. Gilmore, Stephen A. Kelly
Summary: The study found that ILs with longer alkyl chains exhibited greater biocidal activity at relevant concentrations, comparable to commonly used biocides. The primary cytotoxic mechanism was determined to be necrosis, with apoptosis observed at lower concentrations. The modification of anion significantly affected cytotoxicity, indicating that cytotoxicity may be a tuneable property when using ILs as biocides.
Article
Microbiology
Thomas P. Thompson, Stephen A. Kelly, Timofey Skvortsov, Gill Plunkett, Alastair Ruffell, John E. Hallsworth, Jason Hopps, Brendan F. Gilmore
Summary: This study characterized the microbiology of a NaCl stalactite (‘salticle’) in a Triassic halite mine, revealing a unique microbial composition with higher abundance of halophilic microorganisms compared to nearby brine-pools and soil. The metagenomic analysis showed a prevalence of genes involved in osmoregulation, anaerobic respiration, UV resistance, oxidative stress, and stress-protein synthesis in the salticle, indicating its adaptation to extreme environmental conditions. The potential astrobiological implications of salticles as protected salt-saturated habitats with stable water activity were discussed.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Chemistry, Medicinal
Stephen A. Kelly, Jonathan Nzakizwanayo, Aoife M. Rodgers, Li Zhao, Rebecca Weiser, Ismaiel A. Tekko, Helen O. McCarthy, Rebecca J. Ingram, Brian Jones, Ryan F. Donnelly, Brendan F. Gilmore
Summary: This study found that antibiotic treatment can lead to gut microbiota dysbiosis, with the impact of different administration routes needing further investigation. Intravenous delivery may play a role in reducing long-term gut dysbiosis.
ACS INFECTIOUS DISEASES
(2021)
Article
Chemistry, Multidisciplinary
Dilidaer Yusufu, Erin Magee, Brendan Gilmore, Andrew Mills
Summary: The described colour-based carbon dioxide indicator provides an indirect response to aerobic microbial colonization in wounds when used with an occlusive dressing, potentially serving as an early warning indicator for infection in chronic wounds.
CHEMICAL COMMUNICATIONS
(2022)
Article
Microbiology
Cormac J. Rice, Stephen A. Kelly, Seamus C. O'Brien, Erinn M. Melaugh, Jan C. B. Ganacias, Zheng Hua Chai, Brendan F. Gilmore, Timofey Skvortsov
Summary: Proteus mirabilis adheres to urinary catheters and forms biofilms, leading to blockage and decreased antibiotic effectiveness, potentially causing antibiotic resistance. Bacteriophages and their enzymes are being explored as alternative treatment options.
Article
Pathology
Harriet Ravenscroft, Ikhlas El Karim, Anna D. Krasnodembskaya, Brendan Gilmore, Imad About, Fionnuala T. Lundy
Summary: The study revealed that the secretome of dental pulp multipotent mesenchymal stromal cells (MSCs) possesses direct antibacterial activity against various bacteria, including oral pathogens. Cytokines and growth factors present in the secretome were found to contribute to this novel antibacterial activity. This suggests that human dental pulp MSCs play a role in the antibacterial properties of the dental pulp, providing new evidence for the ability of the dental pulp to fight infection and restore functional competence.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Editorial Material
Immunology
Brendan F. F. Gilmore, Diane McDougald, Christophe Beloin
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Integrative & Complementary Medicine
Haroon Iqbal, Claire L. Wright, Sue Jones, Goncalo Rosas da Silva, John McKillen, Brendan F. Gilmore, Owen Kavanagh, Brian D. Green
Summary: This study investigated the immunomodulatory and antibacterial activities of Sida cordifolia. The aqueous extract showed immunomodulatory activity, while the methanolic fraction exhibited antibacterial activity. Sida cordifolia counters bacterial infections through immunomodulatory polysaccharides and antibacterial compounds.
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2022)
Article
Chemistry, Multidisciplinary
Angela L. Tether, Garry Laverty, Alberto Puga, Kenneth R. Seddon, Brendan F. Gilmore, Stephen A. Kelly