Article
Multidisciplinary Sciences
Camilla Luiza-Batista, Sabine Thiberge, Malika Serra-Hassoun, Flore Nardella, Aurelie Claes, Vanessa C. Nicolete, Pierre-Henri Commere, Liliana Mancio-Silva, Marcelo U. Ferreira, Artur Scherf, Sylvie Garcia
Summary: This study presents a new humanized mice model that supports human erythropoiesis and allows the multiplication and transmission of P. vivax parasites. It provides a unique tool for investigating the biology of intraerythrocytic P. vivax.
NATURE COMMUNICATIONS
(2022)
Article
Veterinary Sciences
James C. Tarrant, Zev A. Binder, Mattia Bugatti, William Vermi, Joost van den Oord, Brona Ranieri, Charles-Antoine Assenmacher, Natalie Hoepp, Donald M. O'Rourke, Xiaochuan Shan, Gwenn Danet-Desnoyers, Enrico Radaelli
Summary: Humanized NSGS mice generated from the transplantation of CD34+ hHSC developed a fatal MAS-like phenotype characterized by hepatosplenomegaly, thrombocytopenia, anemia, and disseminated histiocytosis with infiltrates of activated macrophages. The mice exhibited a mixture of human and mouse macrophages in the affected tissues, with inflammasome activation observed in both species. The condition often developed despite low chimerism in peripheral blood, urging caution in long-term studies utilizing huNSGS mice.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Immunology
Takuya Yamaguchi, Ikumi Katano, Iyo Otsuka, Ryoji Ito, Misa Mochizuki, Motohito Goto, Takeshi Takahashi
Summary: This study investigated the role of mouse complement C3 in eliminating human red blood cells, by developing a novel mouse model with a truncated version of the murine C3 gene. Results showed that genetic deletion of C3 prolonged the survival of transfused human red blood cells, potentially paving the way for further research on human diseases associated with red blood cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Ayla Yalamanoglu, Irina L. Dubach, Nadja Schulthess, Giada Ingoglia, Delaney C. Swindle, Rok Humar, Dominik J. Schaer, Paul W. Buehler, David C. Irwin, Florence Vallelian
Summary: Sickle cell disease is a genetic disorder characterized by abnormal red blood cells and vascular occlusion. Acute hepatic crisis can occur under stress conditions, but organ damage can be attenuated by inhibiting certain inflammatory factors.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Biomedical
Miranda M. Carleton, Marius Locke, Michael V. Sefton
Summary: The study demonstrates that MAA-collagen hydrogel significantly enhances skeletal muscle regeneration after traumatic injury, increasing muscle fiber size and force production while reducing pro-inflammatory macrophages. The regenerative effects of the hydrogels are carrier-dependent with distinct gene expression profiles.
Article
Engineering, Biomedical
Georgios Theocharidis, Sahar Rahmani, Sangmin Lee, Zhuqing Li, Antonio Lobao, Konstantinos Kounas, Xanthi-Lida Katopodi, Peng Wang, Salina Moon, Ioannis S. Vlachos, Monika Niewczas, David Mooney, Aristidis Veves
Summary: This study investigates the efficacy of calcium-crosslinked alginate dressings in treating diabetic foot ulcers. The results show that alginate dressings can serve as a delivery platform for primary macrophages and their secretome, accelerating wound healing.
Article
Biotechnology & Applied Microbiology
Yong Cao, Yan Lin, Yan Sun, Weiyu Liu, Yichuan Shao, Changqing Zheng
Summary: The study demonstrated that 4-HPR may be a potent anti-UC agent that works by regulating macrophage polarization via PPAR gamma.
Article
Chemistry, Multidisciplinary
Tiemei Li, Xiaotao Su, Pinglan Lu, Xinmei Kang, Mengyan Hu, Chunyi Li, Shisi Wang, Danli Lu, Shishi Shen, Huipeng Huang, Yuxin Liu, Xiaohui Deng, Wei Cai, Lei Wei, Zhengqi Lu
Summary: Pneumonia is a common cause of death in patients with acute ischemic stroke (AIS), and antibiotics are not effective in improving the prognosis due to the negative impact on the immune system. However, a recent study found that bone marrow mesenchymal stem cells (BM-MSC) can reduce bacterial load in the lungs of stroke mice, and this is achieved by modulating the activities of pulmonary macrophages. The study also identified that BM-MSC releases migrasomes, which contain the antibacterial peptide dermcidin (DCD) and enhance the phagocytosis of macrophages.
Review
Immunology
Patrick Schuhmachers, Christian Munz
Summary: EBV is a highly successful pathogen in humans, infecting over 95% of the adult population, but the immune system is able to control it in most carriers. It is primarily found in lymphomas and epithelial cell carcinomas, with the emergence of EBV-positive tumors requiring additional factors such as co-infections or genetic predispositions. Research in humanized mice provides a valuable platform for testing therapies and vaccines against EBV-associated pathologies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hazel Dunbar, Ian J. Hawthorne, Courteney Tunstead, Michelle E. Armstrong, Seamas C. Donnelly, Karen English
Summary: The expression of macrophage migration inhibitory factor (MIF) is controlled by a functional promoter polymorphism, where the number of tetranucleotide repeats (CATTn) corresponds to the level of MIF expression. Using a pre-clinical model of allergic asthma, novel humanized MIF mice with increasing CATT repeats (CATT5 and CATT7) were employed to study airway inflammation. The results showed that high expression of human MIF in CATT7 mice led to a severe asthma phenotype, which could be mitigated by the MIF inhibitor SCD-19. This study provides a reproducible model for studying asthma airway remodeling and suggests a potential role of MIF in driving this process.
Article
Biochemistry & Molecular Biology
Hazel Dunbar, Ian J. Hawthorne, Courteney Tunstead, Michelle E. Armstrong, Seamas C. Donnelly, Karen English
Summary: Macrophage migration inhibitory factor (MIF) expression is affected by a functional promoter polymorphism, with the number of CATT repeats correlating to MIF expression level. In a pre-clinical model of allergic asthma, mice with increasing CATT repeats (CATT(5) and CATT(7)) showed varying degrees of airway inflammation. The potent MIF inhibitor SCD-19 was able to mitigate the pathophysiology observed in CATT(7) mice, indicating the important role of endogenous human MIF expression in airway inflammation severity.
Article
Biotechnology & Applied Microbiology
Rui Chen, Yujie Li, Yangyang Zhuang, Yiming Zhang, Hailong Wu, Tao Lin, Shixuan Chen
Summary: This study combines GM-CSF and 3D nanofiber scaffolds to promote tissue regeneration. Through subcutaneous implantation in humanized mice, it was found that GM-CSF could accelerate cell migration and cytokine release, and ultimately enhance tissue regeneration. Therefore, GM-CSF loaded 3D nanofiber scaffolds have potential applications in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Immunology
Sang Min Jeon, Young Jae Kim, Thanh Quang Nguyen, Jinsheng Cui, Bui Thi Bich Hanh, Prashanta Silwal, Jin Kyung Kim, Jin-Man Kim, Dong-Chan Oh, Jichan Jang, Eun-Kyeong Jo
Summary: The newly identified cyclic peptide Ohmyungsamycin A (OMS) exhibits significant antimicrobial effects against Mycobacterium tuberculosis and has shown promising results against non-tuberculous mycobacteria (NTMs) infections as well. The study demonstrates that OMS treatment enhances the immune response and promotes proinflammatory reactions against Mycobacteroides abscessus (Mabc) infection in both immunocompetent and immunodeficient mice. Furthermore, the combination of OMS and rifabutin shows a synergistic effect against Mabc infections, suggesting the potential of OMS as an adjunctive therapeutic strategy.
Article
Neurosciences
Jialiang Wei, Shuhui Dai, Chen Pu, Peng Luo, Yuefan Yang, Xiaofan Jiang, Xia Li, Wei Lin, Zhou Fei
Summary: TLR9 plays a role in regulating macrophage/microglial function after intracerebral hemorrhage (ICH). The overexpression of TLR9 facilitates macrophage phagocytosis and protects neurons from blood-derived hazards. This protective effect is mediated through the Nrf2/CD204 pathway. These findings may have implications for ICH treatment.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Article
Immunology
Xinle Cui, Zhouhong Cao, Yuriko Ishikawa, Sara Cui, Ken-Ichi Imadome, Clifford M. Snapper
Summary: EBV core fusion machinery proteins can induce high titer EBV neutralizing antibodies for both B lymphocytes and epithelial cells, with a synergistic effect when gH/gL is combined with trimeric gB. The sera from immunized rabbits not only protect humanized mice from lethal EBV challenge, but also significantly reduce EBV load in peripheral blood. These findings suggest that the combination of gH/gL and trimeric gB could be a promising prophylactic EBV vaccine.
Article
Biochemistry & Molecular Biology
Piotr Ruchala, Alan J. Waring, Marianne Cilluffo, Julian P. Whitelegge, Cameron B. Gundersen
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2019)
Article
Biology
Ewa D. Micewicz, Keisuke S. Iwamoto, Josephine A. Ratikan, Christine Nguyen, Michael W. Xie, Genhong Cheng, Gayle M. Boxx, Elisa Deriu, Robert D. Damoiseaux, Julian P. Whitelegge, Piotr P. Ruchala, Rozeta Avetisyan, Michael E. Jung, Greg Lawson, Elizabeta Nemeth, Tomas Ganz, James W. Sayre, William H. McBride, Dorthe Schaue
RADIATION RESEARCH
(2019)
Correction
Multidisciplinary Sciences
A. A. Shea, R. C. Bernhards, C. K. Cote, C. J. Chase, J. W. Koehler, C. P. Klimko, J. T. Ladner, D. A. Rozak, M. J. Wolcott, D. P. Fetterer, S. J. Kern, G. I. Koroleva, S. P. Lovett, G. F. Palacios, R. G. Toothman, J. A. Bozue, P. L. Worsham, S. L. Welkos
Article
Immunology
Todd M. Kijek, Sherry Mou, Beth A. Bachert, Kathleen A. Kuehl, Janice A. Williams, Sharon P. Daye, Patricia L. Worsham, Joel A. Bozue
MICROBIAL PATHOGENESIS
(2019)
Article
Hematology
Veena Sangkhae, Allison L. Fisher, Kristine J. Chua, Piotr Ruchala, Tomas Ganz, Elizabeta Nemeth
Article
Biochemical Research Methods
Todd M. Kijek, Joel A. Bozue, Rekha G. Panchal, Vladislav A. Litosh, Ronald W. Woodard, S. Ashraf Ahmed
Summary: A user-friendly assay for Arabinose 5-phosphate isomerase (API) activity was developed, monitoring enzymatic reactions in real-time based on circular dichroism (CD) signal changes. The method showed consistent and reproducible results for high-throughput screening applications.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Immunology
Christopher K. Cote, Sergei S. Biryukov, Christopher P. Klimko, Jennifer L. Shoe, Melissa Hunter, Raysa Rosario-Acevedo, David P. Fetterer, Krishna L. Moody, Joshua R. Meyer, Nathaniel O. Rill, Jennifer L. Dankmeyer, Patricia L. Worsham, Joel A. Bozue, Susan L. Welkos
Summary: This study evaluated the safety and efficacy of eight live Y. pestis strains derived from virulent strains CO92 or KIM6+, and identified two safest and most protective vaccine candidates. These vaccines demonstrated high protection against plague in mouse experiments.
Article
Microbiology
Beth A. Bachert, Joshua B. Richardson, Kevin D. Mlynek, Christopher P. Klimko, Ronald G. Toothman, David P. Fetterer, Andrea E. Luquette, Kitty Chase, Jessica L. Storrs, Ashley K. Rogers, Christopher K. Cote, David A. Rozak, Joel A. Bozue
Summary: Researchers have characterized a panel of virulent F. tularensis strains for vaccine testing, finding high genomic similarity but also unique mutations and unexpected diversity within some strains. These findings indicate the effectiveness of the strains as challenge strains for future vaccine development efforts.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Sergei Biryukov, Jennifer L. Dankmeyer, Zain Shamsuddin, Ivan Velez, Nathaniel O. Rill, Raysa Rosario-Acevedo, Christopher P. Klimko, Jennifer L. Shoe, Melissa Hunter, Michael D. Ward, Lisa H. Cazares, David P. Fetterer, Joel A. Bozue, Patricia L. Worsham, Christopher K. Cote, Kei Amemiya
Summary: Research shows that by incorporating multiple toll-like receptor agonists into the F1-V plague vaccine regimen, it is possible to further enhance the immune response, optimize, and augment vaccine efficacy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Kevin D. Mlynek, Christopher T. Lopez, David P. Fetterer, Janice A. Williams, Joel A. Bozue
Summary: This study reports the robust biofilm development observed in a stochastic manner in Type A and B isolates of Francisella tularensis. The frequency of biofilm formation increased over time and exhibited a switch-like behavior. Additionally, pH was found to independently initiate biofilm formation. The study also discovered that isolates with constitutive biofilm formation characteristics delay the onset of a viable non-culturable state.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Raysa Rosario-Acevedo, Sergei S. Biryukov, Joel A. Bozue, Christopher K. Cote
Summary: Plague, caused by the bacterial pathogen Yersinia pestis, is a vector-borne disease that continues to infect humans worldwide. Research on vaccines and therapeutics for plague is important for infection mitigation and disease treatment, as plague remains a public health threat and biodefense concern.
Review
Microbiology
Kevin D. Mlynek, Joel A. Bozue
Summary: The study focuses on the high-consequence human pathogen Francisella tularensis, which has been detected in various hosts and vectors. The study explores the phase variation of the O-antigen and its link to biofilm formation and environmental persistence. Understanding these mechanisms could lead to new medical countermeasures for treating tularemia.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Christopher K. Cote, Jessica M. Weidner, Christopher Klimko, Ashley E. Piper, Jeremy A. Miller, Melissa Hunter, Jennifer L. Shoe, Jennifer C. Hoover, Brian R. Sauerbry, Tony Buhr, Joel A. Bozue, David E. Harbourt, Pamela J. Glass
Summary: The study validated the use of bleach for effective decontamination of spores in complex matrices, with results showing that a certain free chlorine concentration and contact time can achieve the desired effect. Modifications to the system and its operation were necessary when translating the results to validation of the bleach-based chemical EDS.
Article
Microbiology
Fabrice V. Biot, Beth A. Bachert, Kevin D. Mlynek, Ronald G. Toothman, Galina I. Koroleva, Sean P. Lovett, Christopher P. Klimko, Gustavo F. Palacios, Christopher K. Cote, Jason T. Ladner, Joel A. Bozue
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Immunology
Jennifer Chua, Joel A. Bozue, Christopher P. Klimko, Jennifer L. Shoe, Sara Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, Christopher K. Cote
EMERGING INFECTIOUS DISEASES
(2019)