Article
Microbiology
Xinghong Zhao, Xinyi Zhong, Shinong Yang, Kai Deng, Lu Liu, Xu Song, Yuanfeng Zou, Lixia Li, Xun Zhou, Renyong Jia, Juchun Lin, Huaqiao Tang, Gang Ye, Jianqing Yang, Shan Zhao, Yifei Lang, Hongping Wan, Zhongqiong Yin, Oscar P. Kuipers
Summary: Brevicidine, a bacterial cyclic lipopeptide, exhibits potent and selective antimicrobial activity against Gram-negative pathogens. However, little was known about its mechanism of action before our investigations. We found that brevicidine exerts its bactericidal activity by interacting with lipopolysaccharide, targeting phosphatidylglycerol and cardiolipin, and disrupting the proton motive force of bacteria.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Plant Sciences
Igor Pottosin, Miguel Olivas-Aguirre, Oxana Dobrovinskaya, Isaac Zepeda-Jazo, Sergey Shabala
Summary: This study critically discusses the direct and indirect effects of natural polyamines and their catabolites on the activity of key plant ion-transporting proteins, particularly in stress responses. The research predicts the role of polyamines in important cell ionic homeostasis and signaling, and summarizes several unresolved issues including the subcellular levels of polyamines and their mechanisms of action.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Physiology
Kelsy Moffatt, Mark Rossi, Edward Park, Jon Christian Svendsen, Jonathan M. Wilson
Summary: Stomach acid secretion is important for digestion and growth, and can be simulated by using omeprazole, a gastric proton pump inhibitor, to study the effects on metabolism and growth.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
He Zhang, Shengchun Wang, Xiaoyu Wang, Pengjie Wang, Hong Yi, Heng Zhang, Aiwen Lei
Summary: By utilizing inexpensive K2S2O8 as the oxidant at room temperature, the phenoxazination/phenothiazination of electron-rich anilines has been successfully achieved. This catalyst-free method demonstrates complete para-selective amination, showing high efficiency and simplicity in flow-chemistry synthesis.
Review
Medicine, General & Internal
Qi-Yu Zhang, Zhi-Bin Yan, Yue-Ming Meng, Xiang-Yu Hong, Gang Shao, Jun-Jie Ma, Xu-Rui Cheng, Jun Liu, Jian Kang, Cai-Yun Fu
Summary: Antimicrobial peptides (AMPs) are a promising candidate to combat antibiotic resistant bacteria, showing broad-spectrum antimicrobial activity with low toxicity and utilizing sophisticated mechanisms of action. This comprehensive review provides an overview of the origin, structural characteristics, mechanisms of action, biological activities, and clinical applications of AMPs. Strategies to optimize and develop AMP-based treatments for potential antimicrobial and anticancer therapeutics are also discussed.
MILITARY MEDICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Takuto Fujii, Kenji Sugimoto, Takafumi Noda, Takahiro Shimizu, Yuji Matsuya, Hideki Sakai
Summary: A newly synthesized dihydropyrazole derivative KYY-008 was found to selectively inhibit the activity of gastric H+,K+-ATPase, with no effect on other P2-type ATPases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yongqing Yang, Xiaohui Liu, Wei Guo, Wei Liu, Wei Shao, Jun Zhao, Junhong Li, Qing Dong, Liang Ma, Qun He, Yingzhang Li, Jianyong Han, Xiaoguang Lei
Summary: Auxin, a plant hormone, requires polarized transport across plant cells for its function. The study found that PM H+-ATPases play a crucial role in polar auxin transport. A small molecule inhibitor of PM H+-ATPase activity was identified through a chemical screen, confirming the importance of this enzyme in auxin transport.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Matthew R. Blackburn, Benjamin B. Minkoff, Michael R. Sussman
Summary: Over the past two decades, mass spectrometry-based proteomics technologies have advanced the study of signaling pathways, particularly in plants with complex gene families. Newly emerging MS-based techniques offer opportunities to study the 3D structure of proteins in complex mixtures and even intact cells, providing insights into the molecular underpinnings of plant structure and function.
Article
Multidisciplinary Sciences
Rong Wang, Yu Qin, Xiao-Song Xie, Xiaochun Li
Summary: This study identifies mEAK7 as an endogenous modulator of V-ATPase and determines its structure in complex with V-ATPase. The findings suggest that mEAK7 may function as an inhibitor of V-ATPase and potentially link mTOR signaling with V-ATPase activity.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Xueqin Lv, Ke Jin, Yu Yi, Lingang Song, Xiang Xiu, Yanfeng Liu, Jianghua Li, Guocheng Du, Jian Chen, Long Liu
Summary: By visual observation and order analysis of membrane microdomains of Saccharomyces cerevisiae, it was found that membrane microdomains play a crucial role in acid tolerance. This study proposed a new acid tolerance mechanism, providing a new direction for the rational engineering of acid-tolerant strains.
MICROBIAL CELL FACTORIES
(2023)
Review
Plant Sciences
F. J. Alvarez-Martinez, E. Barrajon-Catalan, M. Herranz-Lopez, V Micol
Summary: Plant-derived antimicrobial compounds have shown promising potential in combating bacterial resistance, with polyphenols and terpenes being the most active compounds and plasma membrane disruption as the common mechanism of action. Combinations with beta-lactam antibiotics have been the focus of research in recent years.
Article
Multidisciplinary Sciences
Dan-Dan Chen, Zhao-Bin Wang, Le-Xuan Wang, Peng Zhao, Cai-Hong Yun, Lin Bai
Summary: In this study, the authors determine cryo-EM structures of a chitin synthase, revealing its activation, catalytic, and inhibitory mechanisms.
NATURE COMMUNICATIONS
(2023)
Review
Chemistry, Medicinal
Sudipta Paul Bhattacharya, Snigdha Karmakar, Kusumita Acharya, Arijit Bhattacharya
Summary: The development of biofilms is a protective strategy used by bacterial pathogens against the immune response and antimicrobial treatments. Quorum sensing, which alters gene expression profiles, has been identified as a key regulator of biofilm dynamics. With the rapid emergence of antimicrobial resistance, it is urgent to find alternative interventions to control biofilm infections. Exploring phytochemical products has shown promise in inhibiting quorum sensing and preventing biofilm formation. Recent studies have focused on triterpenoids and their derivatives, which have been found to disrupt quorum sensing and impair biofilm formation against bacterial pathogens.
Article
Food Science & Technology
Jirayu Buatong, Ajay Mittal, Pimonsri Mittraparp-arthorn, Suriya Palamae, Jirakrit Saetang, Soottawat Benjakul
Summary: The antibacterial effect of chitooligosaccharide conjugated with five different polyphenols against Listeria monocytogenes was investigated. Among all the conjugates tested, COS-EGCG showed the highest inhibition toward Listeria monocytogenes, with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 1024 μg/mL, respectively. The COS-EGCG conjugate also had a bactericidal effect on the environmental and clinical strains of L. monocytogenes.
Article
Chemistry, Multidisciplinary
Zizhen Zeng, Jibao Zhu, Xiaoyu Deng, Huanwen Chen, Yi Jin, Emeric Miclet, Valerie Alezra, Yang Wan
Summary: A new concept for reversible peptide stapling has been developed, which involves macrocyclization and decyclization using dual 1,4-elimination. This strategy enables selective peptide delivery to intracellular or extracellular targets. The study also demonstrates the application of reversibly stapled peptides and the activation of their biological activity through dual 1,4-elimination. These findings contribute to significant advancements in the field of peptide delivery.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
M. Acosta-Zaldivar, M. T. Andres, A. Rego, C. S. Pereira, J. F. Fierro, M. Corte-Real
Article
Microbiology
Maria T. Andres, Maikel Acosta-Zaldviar, Jose F. Fierro
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2016)
Article
Food Science & Technology
Manuel Sanchez, Lorena Suarez, Maria Teresa Andres, Blanca Henar Florez, Javier Bordallo, Sabino Riestra, Begona Cantabrana
FOOD & NUTRITION RESEARCH
(2017)
Article
Microbiology
Maria T. Andres, Monica Viejo-Diaz, Jose F. Fierro
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2008)
Article
Biochemistry & Molecular Biology
Maria T. Andres, Maikel Acosta-Zaldivar, Jessica Gonzalez-Seisdedos, Jose F. Fierro
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Catia Santos-Pereira, Maria T. Andres, Susana R. Chaves, Jose F. Fierro, Hernani Geros, Stephen Manon, Ligia R. Rodrigues, Manuela Corte-Real
Summary: The bioactive milk-derived protein lactoferrin exerts its antifungal activity by disrupting lipid rafts and inhibiting the activities of Pma1p and V-ATPase. The findings suggest a potential powerful antifungal approach by combining conventional antifungals with compounds that disrupt lipid rafts.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Nannette Y. Yount, Maria T. Andres, Jose F. Fierro, Michael R. Yeaman
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2007)
Article
Microbiology
MT Andrés, M Viejo-Diaz, F Pérez, JF Fierro
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2005)