Article
Infectious Diseases
Maiken Cavling Arendrup, Sevtap Arikan-Akdagli, Karin Meinike Jorgensen, Aleksandra Barac, Jorg Steinmann, Cristina Toscano, Valentina Arsic Arsenijevic, Assunta Sartor, Cornelia Lass-Floerl, Axel Hamprecht, Tadeja Matos, Benedict R. S. Rogers, Inmaculada Quiles, Jochem Buil, Volkan Ozenci, Robert Krause, Matteo Bassetti, Laura Loughlin, Blandine Denis, Anna Grancini, P. Lewis White, Katrien Lagrou, Birgit Willinger, Riina Rautemaa-Richardson, Petr Hamal, Beyza Ener, Tugce Unalan-Altintop, Ebru Evren, Suleyha Hilmioglu-Polat, Yasemin Oz, Ozlem Koyuncu Ozyurt, Faruk Aydin, Filip Ruzicka, Eelco F. J. Meijer, Jean Pierre Gangneux, Deborah E. A. Lockhart, Nina Khanna, Clare Logan, Ulrike Scharmann, Guillaume Desoubeaux, Emmanuel Roilides, Alida Fe Talento, Karin van Dijk, Philipp Koehler, Jon Salmanton-Garcia, Oliver A. Cornely, Martin Hoenigl
Summary: This study assessed the distribution and antifungal resistance of Candida spp. in candidemia across Europe. The findings showed variations in the types of Candida species and drug resistance among different centers and countries. Cross-resistance to fluconazole and voriconazole was common in Candida glabrata and Candida parapsilosis, but with different geographical prevalence.
JOURNAL OF INFECTION
(2023)
Article
Microbiology
Nancy E. Scott, Serin Edwin Erayil, Susan E. Kline, Anna Selmecki
Summary: The rare Candida (Clavispora) lusitaniae species can rapidly acquire multidrug resistance and cause life-threatening infections in hospital settings. However, the mutations causing this antifungal drug resistance are poorly understood. In a study involving an individual patient, it was found that the evolution of multidrug resistance in C. lusitaniae can occur during treatment with first-line antifungal therapy, and an ERG3 gene mutation was associated with cross-resistance to multiple drug classes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Yating Ning, Meng Xiao, David S. Perlin, Yanan Zhao, Minya Lu, Yi Li, Zhengyu Luo, Rongchen Dai, Shengjie Li, Jiajun Xu, Lingli Liu, Hong He, Yun Liu, Fushun Li, Yuguang Guo, Zhongju Chen, Yingchun Xu, Tianshu Sun, Li Zhang
Summary: Candida parapsilosis is a significant cause of invasive candidiasis in China. Echinocandins are preferred for treatment and prevention of this infection. However, resistance to echinocandins in C. parapsilosis is emerging, and little is known about susceptibility and resistance mechanisms in China.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Microbiology
Maria Siopi, Antonios Papadopoulos, Anastasia Spiliopoulou, Fotini Paliogianni, Nissrine Abou-Chakra, Maiken Cavling Arendrup, Christina Damoulari, Georgios Tsioulos, Efthymia Giannitsioti, Frantzeska Frantzeskaki, Iraklis Tsangaris, Spyros Pournaras, Joseph Meletiadis
Summary: This study describes the isolation of a pan-echinocandin-resistant Candida parapsilosis strain from the urine of a patient who had been exposed to echinocandins for a prolonged period. The isolate harbored a novel alteration in the hotspot 1 region of the fks1 gene. Bloodstream isolates collected from the same patient earlier were susceptible to echinocandins and had wild-type FKS1 sequences.
Article
Microbiology
Maiko Kiyohara, Taiga Miyazaki, Michiyo Okamoto, Tatsuro Hirayama, Koichi Makimura, Hiroji Chibana, Nana Nakada, Yuya Ito, Makoto Sumiyoshi, Nobuyuki Ashizawa, Kazuaki Takeda, Naoki Iwanaga, Takahiro Takazono, Koichi Izumikawa, Katsunori Yanagihara, Shigeru Kohno, Hiroshi Mukae
Summary: Worldwide, there have been outbreaks of invasive infections caused by multidrug-resistant Candida auris, which has a high mortality rate. This study identified a novel resistance mutation (G4061A inducing R1354H) in the FKS1 gene of a caspofungin-resistant clinical isolate. Through the CRISPR-Cas9 system, a recovered strain (H1354R) was generated, in which only this single nucleotide mutation was reverted to its wild-type sequence. The study also analyzed the antifungal susceptibility of mutant strains with only the R1354H mutation introduced into C. auris wild-type strains.
Article
Microbiology
Kathrin Spettel, Sonia Galazka, Richard Kriz, Iris Camp, Birgit Willinger
Summary: This study aimed to analyze whether mutations in FKS1 hotspot regions are associated with micafungin resistance in C. albicans isolates. The results showed that no missense mutations were detected in FKS1 hotspot regions in any of the 115 isolates studied, indicating that resistance conferred by alteration of FKS1 seems unlikely.
Article
Biochemistry & Molecular Biology
Yushuai Mao, Ziyang Zhang, Jinghan Shen, Xiaoru Yin, Tianshi Wang, Xuanming Zheng, Guilin Sheng, Yiqiang Cai, Yingchun Shen, Yuanyuan Chen, Mingguo Zhou, Yabing Duan
Summary: This study reveals intrinsic resistance of F. solani to Phenamacril due to specific differences in myosin5 protein at positions 218 and 376. Mutants carrying substitutions at these positions demonstrate reduced sensitivity to Phenamacril, while exhibiting varied changes in growth and sporulation.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Microbiology
Milena Kordalewska, Geselle Cancino-Prado, Joao Nobrega de Almeida Jr, Igor Brasil Brandao, Renata Tigulini de Souza Peral, Arnaldo. L. L. Colombo, David. S. S. Perlin
Summary: We investigated the echinocandin susceptibility and FKS1 genotypes in clinical isolates of Candida auris from patients in Salvador, Brazil. Three isolates with a novel FKS1 mutation were found to be resistant to echinocandins, causing elevated minimum inhibitory concentrations. Introduction of the Fks1 W691L mutation into susceptible strains also resulted in increased resistance to echinocandins.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Rohan Dandage, Caroline M. Berger, Isabelle Gagnon-Arsenault, Kyung-Mee Moon, Richard Greg Stacey, Leonard J. Foster, Christian R. Landry
Summary: The study investigated protein-protein interactions in hybrids between yeast species, finding that most interactions are similar to those of the parents but with some altered instances. It suggests that the occurrence of chimeric protein complexes is frequent, potentially due to incompatibilities or imbalances between parental proteomes.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Plant Sciences
Mei Wang, Weixiao Tang, Li Xiang, Xuesen Chen, Xiang Shen, Chengmiao Yin, Zhiquan Mao
Summary: This study investigated the transcriptional response of apple seedlings inoculated with arbuscular mycorrhizal fungi to infection with F. solani. The results showed that mycorrhizal apple plants had less damage to their roots when infected with F. solani, along with enhanced activity of antioxidant enzymes and reduced lipid oxidation. A total of 1839 differentially expressed genes (DEGs) were identified after infection, with most of the DEGs involved in the binding of ADP and calcium ions. Furthermore, a large number of DEGs were found to be involved in the stress response of mycorrhizal plants based on MapMan analysis. Among them, MdWRKY40 transcription factor significantly improved the resistance of apple callus to F. solani by binding the promoter of the resistance gene MdGLU.
Article
Chemistry, Applied
Xu Qian, Hua Zhou, Heng Cai
Summary: UDP-glucose pyrophosphorylase (UGP1) is an important metabolic enzyme in Saccharomyces cerevisiae. Knocking down ugp1 leads to decreased survival rate, trehalose and glucan content, as well as increased sensitivity to cell wall perturbing agents in the yeast strain. Complementing ugp1 can rescue the resistance to heat stress and cell wall perturbing agents, suggesting the essential role of ugp1 in the heat stress response of S. cerevisiae.
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
(2021)
Article
Microbiology
Inaam Al-Obaid, Mohammad Asadzadeh, Suhail Ahmad, Khaled Alobaid, Wadha Alfouzan, Ritu Bafna, Maha Emara, Leena Joseph
Summary: Candida auris is an emerging yeast pathogen that has caused major outbreaks worldwide. This study presents a case of C. auris infection in an immunocompromised patient who had previously received antifungal treatment, and highlights the challenges in treating invasive C. auris infections due to developing drug resistance and novel mutations.
Article
Dermatology
Mohammad Asadzadeh, Eiman Mokaddas, Suhail Ahmad, Aneesa A. Abdullah, Theun de Groot, Jacques F. Meis, Shama A. Shetty
Summary: This study analyzed 49 Candida auris isolates and identified a novel mutation conferring reduced susceptibility to echinocandins. The study also found unfavorable clinical outcomes for most C auris-infected patients.
Article
Biotechnology & Applied Microbiology
Katsuyuki Sakai, Yufu Unten, Aoi Kimishima, Kenichi Nonaka, Takumi Chinen, Kazunari Sakai, Takeo Usui, Kazuro Shiomi, Masato Iwatsuki, Masatoshi Murai, Hideto Miyoshi, Yukihiro Asami, Satoshi Omura
Summary: The study identified two new tetramic acid derivatives, traminines A (1) and B (2), isolated from a culture broth of Fusarium concentricum FKI-7550, with their chemical structures elucidated by NMR studies. The compounds were found to inhibit the growth of a multidrug-sensitive yeast strain by targeting enzymes involved in oxidative phosphorylation in yeast mitochondria. Compound 1 was found to inhibit both complex III and FoF1-ATPase, while compound 2 solely inhibited complex III.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Alexis Garcia, Eun Young Huh, Soo Chan Lee
Summary: Procedures such as solid-organ transplants and cancer treatments can weaken the immune system, making patients more susceptible to opportunistic diseases like fungal infections. Mucormycosis infections, caused by Mucorales fungi, have been increasing, particularly in patients with SARS-CoV-2 infections. These fungi are resistant to most antifungal drugs, including echinocandins, which are currently the most effective. The study found that the resistance to echinocandins in Mucorales fungi is due to the overexpression of fks genes and the role of calcineurin.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)