Review
Pathology
Eleonora Riccobono, Linda Bussini, Maddalena Giannella, Pierluigi Viale, Gian Maria Rossolini
Summary: The management of pneumonia patients involves challenges such as accurate microbiological investigations, prompt therapeutic management, and optimal treatment duration. Rapid diagnostic tests may be a feasible tool to optimize timing of targeted treatments, especially in terms of early de-escalation or discontinuation of antibiotic therapy.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2022)
Editorial Material
Medicine, General & Internal
Alessandro Milia, Silvia Fruttuoso, Antonio Mancini, Eleonora Riccobono, Fabio Luise, Lucia Sammicheli, Gian Maria Rossolini, Filippo Pieralli
INTERNAL AND EMERGENCY MEDICINE
(2022)
Article
Infectious Diseases
Eleonora Riccobono, Tommaso Giani, Giulia Baldi, Sophie Arcangeli, Alberto Antonelli, Valeria Tellone, Alessandra Del Vecchio, Alessandra Capezzone De Joannon, Gian Maria Rossolini
Summary: This study evaluated the antimicrobial activity of dalbavancin against Gram-positive pathogens and found that it demonstrated excellent and stable activity, particularly against MRSA. The results indicated a low propensity for resistance selection and highlighted the potential efficacy of dalbavancin in treating ABSSSI caused by Gram-positive bacteria.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Selene Rebecca Boncompagni, Maria Micieli, Tiziana Di Maggio, Noemi Aiezza, Alberto Antonelli, Tommaso Giani, Gloria Padoani, Silvia Vailati, Lucia Pallecchi, Gian Maria Rossolini
Summary: This study investigated the activity of fosfomycin, colistin, and their combination against multidrug-resistant Gram-negative pathogens. The results showed that the combination of fosfomycin and colistin exhibited synergistic effects against biofilms and reduced the emergence of drug-resistant subpopulations.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Daniela Cejas, Alan Elena, Francisco E. Gonzalez-Espinosa, Lucia Pallecchi, Carlos Vay, Gian Maria Rossolini, Gabriel Gutkind, Vincenzo Di Pilato, Marcela Radice
Summary: This study describes two novel plasmids harboring bla(KPC-2) in Pseudomonas aeruginosa from Latin America, which have different structures and original contexts for bla(KPC-2). The research expands the knowledge about the molecular epidemiology of bla(KPC-2) in P. aeruginosa.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Review
Infectious Diseases
Paolo Gaibani, Tommaso Giani, Federica Bovo, Donatella Lombardo, Stefano Amadesi, Tiziana Lazzarotto, Marco Coppi, Gian Maria Rossolini, Simone Ambretti
Summary: Multidrug resistance (MDR) is a serious global threat due to the rapid spread of MDR pathogens and limited treatment options. New beta-lactams/beta-lactamase inhibitor combinations (beta L-beta LICs) have shown promise for treating MDR Gram-negative infections. However, resistance to these novel molecules has started to emerge. This review summarizes the mechanisms of resistance to ceftazidime/avibactam, meropenem/vaborbactam, and imipenem/relebactam in MDR Gram-negative microorganisms, with a focus on antimicrobial activities and resistance traits related to novel beta L-PLICs. The detection methods for antimicrobial susceptibility testing of these molecules are also discussed.
Article
Infectious Diseases
Selene Rebecca Boncompagni, Maria Micieli, Tiziana Di Maggio, Antonia Mantella, Anna Liz Villagran, Tatiana Briggesth Miranda, Carmen Revollo, Veronica Poma, Herlan Gamboa, Michele Spinicci, Marianne Strohmeyer, Alessandro Bartoloni, Gian Maria Rossolini, Lucia Pallecchi
Summary: This study examined the fecal carriage of CTX-M-producing Enterobacterales in school-aged children from rural areas of the Bolivian Chaco. The results showed a significant increase in the fecal carriage of CTX-M-producing Enterobacterales from 2016 to 2019. The study highlights the importance of understanding the dissemination dynamics of this resistance determinant.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Medicine, General & Internal
Giulia Mandelli, Francesca Dore, Martin Langer, Elena Garbero, Laura Alagna, Andrea Bianchin, Rita Ciceri, Antonello Di Paolo, Tommaso Giani, Aimone Giugni, Andrea Gori, Ugo Lefons, Antonio Muscatello, Carlo Olivieri, Angelo Pan, Matteo Pedeferri, Marianna Rossi, Gian Maria Rossolini, Emanuele Russo, Daniela Silengo, Bruno Viaggi, Guido Bertolini, Stefano Finazzi
Summary: This study demonstrates the successful implementation of an antibiotic stewardship program in seven Italian intensive care units. The program effectively improved infection management by reducing the duration of empirical therapy, lowering the use of quinolones, and decreasing the proportion of multi-drug-resistant bacteria in ICU-acquired infections. ICU mortality and length of stay remained unchanged, indicating that patient safety was not compromised by reducing antibiotic administration.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Microbiology
Felice Valzano, Selene Rebecca Boncompagni, Maria Micieli, Tiziana Di Maggio, Vincenzo Di Pilato, Lorenzo Colombini, Francesco Santoro, Gianni Pozzi, Gian Maria Rossolini, Lucia Pallecchi
Summary: Chronic colonization by Pseudomonas aeruginosa is critical in cystic fibrosis (CF) and other chronic lung diseases. Evaluation of combination therapies for treating biofilm-associated P. aeruginosa lung infections has been of growing interest. N-acetylcysteine (NAC) alone has limited antibiofilm activity, but shows synergistic effects when combined with colistin. NAC exposure induces a Zn2+ starvation response, downregulates denitrification genes, and reduces flagellum-mediated motility in P. aeruginosa. NAC-colistin combinations may be effective in managing biofilm-associated P. aeruginosa lung infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Gian Maria Rossolini, Gregory Stone, Michal Kantecki, Francis F. Arhin
Summary: This study assessed the in vitro activity of the drug combination aztreonam/avibactam (ATM-AVI) against drug-resistant Enterobacterales, including strains producing metallo-beta-lactamases (MBLs). The results demonstrated that ATM-AVI exhibited potent antimicrobial activity against Enterobacterales, including MBL-producing strains. ATM-AVI represents a potential option for treating infections caused by antibiotic-resistant Enterobacterales, including MBL-producing strains.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Microbiology
Paola Morici, Cosmeri Rizzato, Emilia Ghelardi, Gian Maria Rossolini, Antonella Lupetti
Summary: This study describes the synergistic effect between rifampicin and hLF1-11 against multidrug-resistant Klebsiella pneumoniae. The results suggest that hLF1-11 induces a transient loss of membrane potential and increased cell membrane permeability, allowing rifampicin to enter the bacterial cell.
MICROBIOLOGY SPECTRUM
(2023)
Editorial Material
Medicine, General & Internal
Daniele Roberto Giacobbe, Vincenzo Di Pilato, Ilias Karaiskos, Tommaso Giani, Anna Marchese, Gian Maria Rossolini, Matteo Bassetti
Summary: Antimicrobial resistance, particularly carbapenem resistance in Gram-negative bacteria, poses a significant global health threat. The emergence of carbapenemase-producing Klebsiella pneumoniae (KPC-Kp) has presented clinicians with unique challenges in managing severe infections. This article discusses the evolution of treatment strategies for KPC-Kp infections over the past decade, guided by clinical evidence and advances in diagnostics.
ANNALS OF MEDICINE
(2023)
Article
Infectious Diseases
Anna Carannante, Paola Vacca, Stefano Fontana, Ivano Dal Conte, Valeria Ghisetti, Marco Cusini, Grazia Prignano, Caterina Vocale, Anna Maria Barbui, Elena Stroppiana, Marina Busetti, Antonella Mencacci, Marina Rotondi, Maria Antonia De Francesco, Carmen Luciana Bonanno, Patrizia Innocenti, Maria Agnese Latino, Eleonora Riccobono, Federica Poletti, Ines Clotilde Casonato, Graziella Soldato, Luigina Ambrosio, Stefano Boros, Andrea Ciammaruconi, Florigio Lista, Paola Stefanelli
Summary: This study analyzed the trend of antimicrobial resistance in a 7-year isolate collection in Italy and found an increasing resistance to ceftriaxone monotherapy or ceftriaxone plus azithromycin. It also observed a rise in azithromycin and ciprofloxacin resistance. These findings are important for monitoring the spread of drug-resistant gonorrhea.
MICROBIAL DRUG RESISTANCE
(2023)
Letter
Infectious Diseases
Nicla Giovacchini, Alberto Antonelli, Elia Taddei, Tommaso Giani, Gian Maria Rossolini
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)