期刊
ANTARCTIC SCIENCE
卷 23, 期 6, 页码 549-555出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0954102011000393
关键词
Antarctica; DGGE; South Shetland Islands; thermophiles
资金
- Instituto Antartico Chileno [G01-07]
Deception Island, an active stratovolcano located in the South Shetland Islands, Antarctica, provides excellent conditions for the thermophilic bacteria growth because of high ground temperatures in specific areas, such as Fumarole Bay where the temperatures are above the mesophilic range. Denaturing Gradient Gel Electrophoresis (DGGE) was used with the 16S ribosomal gene to analyse cultures of thermophilic bacteria from a soil sample taken from Fumarole Bay. Nine bands were sequenced and analysed from DGGE and they indicated the presence of bacteria from the genera Geobacillus, Bacillus, Brevibacillus, Thermus and uncultured sulphate reducing bacteria. Some of which have been reported in other Antarctic geothermal sites. Geobacillus, Bacillus and Brevibacillus genera were successfully cultivated in an enriched medium. A pure culture of one thermophilic Geobacillus bacterium was obtained closely related to Geobacillus jurassicus.
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