期刊
ANNUAL REVIEW OF BIOCHEMISTRY
卷 77, 期 -, 页码 77-100出版社
ANNUAL REVIEWS
DOI: 10.1146/annurev.biochem.77.061206.174353
关键词
RNA folding kinetics; single molecule; force unfolding; FRET; helicase; translation
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM010840, R01GM010840] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM010840-49, R01 GM010840, GM 10840, R01 GM010840-50] Funding Source: Medline
Understanding how RNA folds and what causes it to unfold has become more important as knowledge of the diverse functions of RNA has increased. Here we review the contributions of single-molecule experiments to providing answers to questions such as: How much energy is required to unfold a secondary or tertiary structure? How fast is the process? How do helicases unwind double helices? Are the unwinding activities of RNA-dependent RNA polymerases and of ribosomes different from other helicases? We discuss the use of optical tweezers to monitor the unfolding activities of helicases, polymerases, and ribosomes, and to apply force to unfold RNAs directly. We also review the applications of fluorescence and fluorescence resonance energy transfer to measure RNA dynamics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据