Thalassocola ureilytica gen. nov., sp nov., of the family Phyllobacteriaceae isolated from seawater

A Gram-stain-negative, aerobic, rod-shaped, non-flagellated marine bacterium, designated strain LS-861(T), was isolated from seawater of the South China Sea (Taiwan). Strain LS-861(T) grew optimally at pH 7.0 and 30 degrees C in the presence of 3 % (w/v) NaCl. The novel strain shared highest 16S rRNA gene sequence similarity (91.5 % each) with 'Nitratireductor shengliensis' CGMCC 1.12519 and Hoeflea halophila JG120-1(T) and lower sequence similarity (<91.5 %) with other species. Phylogenetic analyses based on 16S rRNA gene sequences revealed a distinct taxonomic position attained by strain LS-861(T) within the clade that accommodated members of the family Phyllobacteriaceae. The major fatty acids were C-16 : 0, iso-C-17 : 1 omega 10c, C-18 : 0 3-OH and C-18 : 1 omega 7c/C-18 : 1 omega 6c. The polar lipid profile was relatively simple as compared with other representatives of Phyllobacteriaceae, by having major amounts of diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid, and moderate amounts of three unidentified phospholipids and an unidentified aminolipid. The DNA G+C content was 61.2 mol%. The predominant quinone system was ubiquinone-10 (Q-10). The data in general and phylogenetic and polar lipid data in particular clearly distinguish the novel strain from related species at the genus level. Thus, strain LS-861(T) is suggested to represent a novel species of a new genus of the family Phyllobacteriaceae, for which the name Thalassocola ureilytica gen. nov., sp. nov. is proposed. The type strain of Thalassocola ureilytica is LS-861(T) (= BCRC 80818(T) = JCM 30682(T)).