Mameliella atlantica sp nov., a marine bacterium of the Roseobacter clade isolated from deep-sea sediment of the South Atlantic Ocean

A taxonomic study was carried out on strain L6M1-5(T), which was isolated from deep-sea sediment collected from the South Atlantic Ocean. The isolate was Gram-reaction-negative, oxidase-negative and catalase-weakly positive. Growth was observed in the presence of 0.5-15% (w/v) NaCl (optimum 3-5 %), at 10-41 degrees C (optimum 28-30 degrees C), and pH 5.0-10.5 (optimum pH 7.0). The principal fatty acids were summed feature8 (C-18:1 omega 7c/omega 6c) (84.2%), C-18:0 (6.3%), C-12:1 3-OH (3.2 %) and C-16:0 (2.7%). The polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, two unknown phospholipids and one unknown lipid. Ubiquinone-10 was the major quinone. The G + C content of the genomic DNA was 66.0 mol %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain L6M1-5(T) belonged to the genus Mameliella and shared 95.8 % sequence similarity with Mameliella alba JL1354-W-T. The combined genotypic and phenotypic data show that strain L6M1-5(T) represents a novel species of the genus Mameliella, for which the name Mameliella. atlantica sp. nov. is proposed. The type strain is L6M1-5(T) (=MCCC 1A07531(T)=JCM 30230(T)).