Defluviimonas alba sp nov., isolated from an oilfield

Two Gram-stain-negative, rod-shaped bacterial strains, cai42(T) and b45, were isolated from oil-production water taken from Xinjiang Oilfield, China. Optimum growth was observed at 30 degrees C, at pH 8 and with 1-3% (w/v) NaCI. According to phylogenetic analyses, the two strains were members of the genus Defluviimonas, with 16S rRNA gene sequence similarities of 95.5-96.3% with the type strains of species of the genus. The major cellular fatty acids of strains cai42(T) and b45 were C-10:0 3-OH, C-16:0 and summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c), and the predominant ubiquinone was Q-10, all of these data being typical for the genus Defluviimonas. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, glycolipid, phosphatidylcholine, two unidentified aminolipids, an unidentified phospholipid and two unidentified lipids. The mean genomic DNA G +C contents of strains cai42(T) and b45 were 60.8 +/- 1.1 and 60.4 +/- 1.0 mol%, respectively. On the basis of phylogenetic, physiological and chemotaxonomic analyses, strains cai42(T) and b45 represent a novel species of the genus Defluviimonas, for which the name Defluviimonas alba sp. nov. is proposed. The type strain is cai42(T) (=CGMCC 1.12518(T)= LMG 27406(T)).