Pseudorhodoplanes sinuspersici gen. nov., sp nov., isolated from oil-contaminated soil

Strain RIPI 110(T) was isolated from a soil sample collected from an oil-contaminated site on Siri Island, Persian Gulf, Iran. Cells of the novel isolate were Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped. Cells divided asymmetrically by budding and formed rosette-like clusters. The optimum pH and temperature for growth were pH 7 and 30 degrees C, while the strain was able to grow at pH 5.5-8 and 15-35 degrees C. Strain RIPI 110(T) utilized only complex carbon sources and pyruvate as the sole carbon source and could not grow under photoautotrophic conditions. The highest 16S rRNA gene sequence similarities, 93.9, 93.9 and 93.5 %, were obtained with Variibacter gotjawalensis GJW-30(T), Rhodoplanes roseus 941(T) and Rhodoplanes elegans AS130(T), respectively. The major cellular fatty acids were summed feature 8 (C-18 : 1 omega 7c/omega 6c), C-16 : (0) and C-19 : 0 cyclo omega 8c. Polar lipid analyses revealed that strain RIPI 110(T) contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid and four unknown phospholipids. Ubiquinone-10 was the predominant quinone component. The DNA G+C content was 59.4 mol%. On the basis of the 16S rRNA gene sequence analysis, in combination with chemotaxonomic and physiological data, the novel isolate could not be classified in any recognized genera. Strain RIPI 110(T) is thus considered to represent a novel species of a new genus within the order Rhizobiales, for which the name Pseudorhodoplanes sinuspersici gen. nov., sp. nov. is proposed. The type strain of the type species is RIPI 110(T) (=IBRC-M 10770(T)=CECT 8374(T)).