Alistipes inops sp nov and Coprobacter secundus sp nov., isolated from human faeces

Culture-based study of the faecal microbiome in two adult female subjects revealed the presence of two obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gram-negative bacterial strains that represent novel species. The first strain, designated 627(T), was a fastidious, slow-growing, indole-positive bacterium with a non-fermentative type of metabolism. The strain was characterized by the production of acetic and succinic acids as metabolic end products, the prevalence of iso-C-15 : 0 fatty acid and the presence of menaquinones MK-10 and MK-11. The DNA G+C content was found to be 56.6 mol%. The second strain, designated 177(T), was capable of fermenting a rich collection of carbohydrate substrates, producing acetic acid as a terminal product. The strain was indole-negative and resistant to bile. The major cellular fatty acids were iso-C-15 : 0 and anteiso-C-15 : 0 (in a 1 : 1 ratio) and the predominant menaquinone was MK-11.The DNA G+C content was 37.8 mol%. A phylogenomic analysis of the draft genomes of strains 627(T) and 177(T) placed these bacteria in the genera Alistipes (family Rikenellaceae) and Coprobacter (family Porphyromonadaceae), respectively. On the basis of the phenotypic and genotypic properties of strains 627(T) and 177(T), we conclude that these strains from human faeces represent two novel bacterial species, for which the names Alistipes Mops sp. nov. (type strain 627(T)=DSM 28863(T)=VKM B-2859(T)) and Coprobacter secundus sp. nov. (type strain 177(T)=DSM 28864(T)=VKM B-2857(T)) are proposed.