期刊
出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1749-6632.2009.04028.x
关键词
methionine; caloric restriction; tight junction; LLC-PK1; mannitol; paracellular; claudin; occludin; sirtuin; diet
The relative abundance of various claudin proteins of LLC-PK1 renal epithelial tight junctions (TJs) is modulated by culturing the cells in a medium that is sharply reduced in the sulfur-containing amino acids, cysteine, cystine, and methionine. The functional result is an epithelial barrier that has a higher transepithelial electrical resistance and a decreased paracellular leak to D-mannitol (i.e., improved barrier function). This is accomplished without affecting the culture's confluent cell density, its short circuit current, or its hallmark differentiated property, Na+-dependent sugar transport. The implications of being able to enhance epithelial TJ barrier function by nutritional means are discussed, particularly in light of the ability of methionine-restrictive diets to enhance life span and forestall age-related morbidity.
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