Article
Microbiology
Yuanyuan Xu, Sabine Ehrt, Dirk Schnappinger, Tiago Beites
Summary: This study elucidates the mechanism of synthetic lethality of NADH dehydrogenases in Mycobacterium tuberculosis. NADH dehydrogenases have the functions of maintaining the NADH/NAD+ ratio and providing electrons to the respiratory chain, and the oxidation of NADH is essential for the viability of Mtb.
Article
Chemistry, Analytical
Yanping Zhao, Xu Geng, Xiaoling Zhou, Li Xu, Shuai Li, Zhengqiang Li, Yi Guo, Chen Li
Summary: A highly stable bio-electrochemical pyruvate sensor was developed for accurately detecting pyruvate levels in blood. The sensor, affixed to a glassy carbon electrode, showed improved stability and sensitivity by employing lactate dehydrogenase and gold nanoparticles-reduced graphene oxide. The sensor exhibited excellent stability and anti-interference ability, outperforming conventional spectroscopic methods for pyruvate detection in artificial serum.
ANALYTICA CHIMICA ACTA
(2023)
Article
Spectroscopy
Yangzhe Zhou, Min Qi, Minghui Yang
Summary: Silicon quantum dots synthesized from 3-aminopropyltrimethoxysilane were used to detect lactate dehydrogenase activity through changes in fluorescence intensity. The assay showed high selectivity and linear calibration, successfully applied in analysis of LDH in human serum samples, with potential for diagnosis and prognosis in various diseases, especially melanoma.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Analytical
Yangzhe Zhou, Min Qi, Minghui Yang
Summary: Clinical impact and principles of lactate dehydrogenase (LDH) detection are systematically summarized in this review. LDH detection is not only correlated with clinical prognostic survival indicators in cancer patients, but also provides guidance for subsequent drug therapy. Additionally, LDH serves as a biomarker for malaria and other diseases. Therefore, the development of sensitive and convenient methods for LDH detection is urgently needed. The review also discusses the advantages, limitations, and future trends in LDH detection.
Article
Chemistry, Multidisciplinary
Yongwoo Jang, Taegyu Park, Eunyoung Kim, Jong Woo Park, Dong Yeop Lee, Seon Jeong Kim
Summary: By utilizing the cellular redox system, a CNT yarn supercapacitor with maximum area capacitance was developed and demonstrated excellent performance under physiological conditions. Implanted NAD/BQ/CNT yarn electrodes in a rat's abdominal cavity showed stable in vivo electrical performance of a supercapacitor. These findings suggest a promising platform for implantable energy storage devices using redox biomolecules.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Shengnan Fan, Xiaoqing Li, Fanghui Ma, Minghui Yang, Juan Su, Xiang Chen
Summary: This study reported a sensitive fluorescence assay for the accurate detection of LDH activity in serum using sulfur quantum dots (SQDs) and nicotinamide adenine dinucleotide (NAD(+)). The method achieved detection of LDH in the range from 500 to 40,000 U L-1 with a limit of detection of 262 U L-1. It has potential clinical applications.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Review
Nutrition & Dietetics
Andrea Benzi, Alessia Grozio, Sonia Spinelli, Laura Sturla, Andreas H. Guse, Antonio De Flora, Elena Zocchi, Joerg Heeren, Santina Bruzzone
Summary: Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in energy metabolism regulation as both a coenzyme and substrate for various enzymes. CD38, as an enzyme synthesizing Ca2+-mobilizing second messenger and a major NAD(+)-consumer, can impact NAD(+) levels. This review explores recent studies on the modulation of CD38 expression and activity, and the resulting changes in NAD(P)(H) levels in adipose tissue.
Article
Biochemistry & Molecular Biology
Cuiting Liao, Li Zhang, Rong Jiang, Juanjuan Xu, Jiarui Tang, Kai Hu, Shifang Jiang, Longhui Li, Yongqiang Yang, Jiayi Huang, Li Tang, Longjiang Li
Summary: The expression of NADK is positively correlated with APAP-induced acute liver injury, and inhibition of NADK can alleviate liver injury by increasing hepatic NAD(+) level and improving antioxidant capacity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Neurosciences
Mariajose Metcalfe, Brian T. David, Brett C. Langley, Caitlin E. Hill
Summary: Spinal cord injury leads to tissue damage and functional deficits. Administering NR can increase NAD+ levels in the injured spinal cord, protect tissue, and improve motor function.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Pharmacology & Pharmacy
Yumeng Zhu, Ping Xu, Xuan Huang, Wen Shuai, Li Liu, Shuai Zhang, Rui Zhao, Xiuying Hu, Guan Wang
Summary: Nicotinamide phosphoribosyltransferase (NAMPT) plays a significant role in mammals by synthesizing nicotinamide mononucleotide (NMN) for metabolic homeostasis and cell survival, serving as a key protein in the host's defense mechanism. Neurodegenerative diseases pose a serious threat to human health, but effective drugs are limited. NAMPT has active biological functions in neurogenesis, making it a powerful therapeutic target for neurodegenerative diseases.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Nutrition & Dietetics
Qin Song, Xiaofeng Zhou, Kexin Xu, Sishi Liu, Xinqiang Zhu, Jun Yang
Summary: Nicotinamide mononucleotide (NMN) is a precursor for NAD+ biosynthesis, and supplementation has shown to increase NAD+ concentration and alleviate aging-related disorders. While most studies have been conducted in cell or animal models, several human clinical trials are currently evaluating the safety and physiological effects of NMN supplementation.
ADVANCES IN NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Gamal Nasser Abdel-Hady, Takeshi Ikeda, Takenori Ishida, Hisakage Funabashi, Akio Kuroda, Ryuichi Hirota
Summary: In this study, a thermotolerant phosphite dehydrogenase from Ralstonia sp. 4506 (RsPtxD) was engineered to create a highly efficient and robust NADPH regeneration system. By changing specific amino acid residues, a mutant RsPtxD(HARRA) with significantly increased preference for NADP was obtained, displaying the highest catalytic efficiency among previously reported phosphite dehydrogenases. This mutant exhibited high thermostability and organic solvent tolerance, making it a promising alternative NADPH regeneration system for practical applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
JuKyung Lee, Han Na Suh, Saeyoung Ahn, Hye Bin Park, Jeong Yoon Lee, Hyung Jin Kim, Sang Hee Kim
Summary: This study developed electrocatalytic sensors for analyzing NADH in whole blood, which exhibited stability and a limit of detection of 3.5 μM. These sensors have the potential for extensive applications.
SCIENTIFIC REPORTS
(2022)
Review
Pharmacology & Pharmacy
Hewang Lee, Pedro A. Jose
Summary: Metabolic syndrome (MetS) is a group of risk factors related to cardiovascular disease and diabetes, characterized by central obesity, hyperglycemia, dyslipidemia, and increased blood pressure. Oxidative stress, primarily caused by the imbalance of pro-oxidant and endogenous antioxidant systems, is the main pathological basis of MetS, with nicotinamide-adenine dinucleotide phosphate (NADPH) oxidases and mitochondria as major sources of reactive oxygen species (ROS) associated with MetS.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Ryo Matsuyama, Tomoyo Omata, Michiharu Kageyama, Ryota Nakajima, Masanobu Kanou, Kei Yamana
Summary: Nicotinamide adenine dinucleotide (NAD(+)) is an essential coenzyme for energy production. Associations between NAD(+) and aging-related diseases have been reported, and NAD(+) precursors are acknowledged as anti-aging supplements. However, there have been few studies on the link between aging or aging-related diseases and human blood NAD(+) concentration due to the instability and difficulty in measuring NAD(+). This study aimed to develop a simpler quantitative NAD(+) measurement method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)