期刊
MAILLARD REACTION: RECENT ADVANCES IN FOOD AND BIOMEDICAL SCIENCES
卷 1126, 期 -, 页码 280-282出版社
BLACKWELL PUBLISHING
DOI: 10.1196/annals.1433.037
关键词
glycation; DNA; methylglyoxal; glyoxal; deoxyguanosine; 8-hydroxydeoxyguanosine; imidazopurinone
资金
- BBSRC [BB/D006295/2] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D006295/2] Funding Source: Medline
- Cancer Research UK [A5147] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
An analytical procedure was developed for nucleotide advanced glycation endproducts formed by the reaction of glyoxal and methylglyoxal with deoxyguanosine under physiological conditions. For this, the imidazopurinone derivatives, 3-(2'-deoxyribosyl)-6,7-dihydro-6,7-dihydroxyimidazo[2,3-b]purin-9(8)one (dG-G) and 3-(2'-deoxyribosyl)-6,7-dihydro-6,7-dihydroxy-6-methylimidazo-[2,3-b]purine-9(8)one (dG-MG), were prepared. Authentic standard and stable isotope-substituted standard adducts were prepared and an isotopic dilution analysis assay methodology was developed using liquid chromatography with tandem mass spectrometry and optimized DNA extraction and nuclease digestion procedures. Analysis of dG-G, dG-MG, and the oxidative marker 8-hydroxydeoxyguanosine in the DNA of cultured human cells and mononuclear leukocytes showed that nucleotide advanced glycation endproducts are major markers of DNA damage in human cells.
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