Article
Environmental Sciences
Ng. Kunjarani Chanu, Madan Kumar Mandal, Akanksha Srivastava, Neha Chaurasia
Summary: The study investigated the effects of pyrethroid insecticide alpha-cypermethrin on cyanobacteria Anabaena sp. NC-K1, revealing upregulation of various proteins involved in stress responses, photosynthesis, translation, and DNA repair. This proteomic modulation allowed Anabaena sp. NC-K1 to maintain cellular metabolism and homeostasis against pesticide stress.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Gao-Xiang Wang, Ze-Bin Fang, Jun-Tong Li, Bao-Li Huang, De-Liang Liu, Shu-Fang Chu, Hui-Lin Li
Summary: This study aimed to investigate the correlation between serum albumin and diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (T2DM). The results showed a significant negative correlation between serum albumin levels and DR, suggesting that serum albumin levels can be used as a reference point for long-term follow-up of patients with T2DM.
Review
Biochemistry & Molecular Biology
Hongwei Song, Hongyan Ma, Junfeng Shi, Yongping Liu, Chengxia Kan, Ningning Hou, Jing Han, Xiaodong Sun, Hongyan Qiu
Summary: Diabetes is a multifactorial disorder that has serious consequences on mortality and disability due to complications resulting from nonenzymatic glycation. This review provides a comprehensive understanding of the molecular mechanisms and pathological consequences of nonenzymatic glycation in diabetes, as well as various strategies to prevent and control glycation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Spectroscopy
Annunziata Lapolla
Summary: This article introduces the collaboration and research progress between the research group in the Department of Medicine at Padua University and the mass spectrometric group at CNR of Padua in the field of nonenzymatic protein glycation. The development of new mass spectrometric techniques has allowed for in-depth investigation of the effects of browning and glycation levels on proteins, revealing the role of advanced glycation end products in diabetes complications.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Plant Sciences
Swetha Sudha Nagamalla, Malini Devi Alaparthi, Sunitha Mellacheruvu, Ravindar Gundeti, Jana Priya Sony Earrawandla, Someswar Rao Sagurthi
Summary: The study investigated the impact of drought stress on two cotton varieties, revealing that Bt cotton showed stronger drought tolerance compared to non-Bt cotton at the protein expression and physiological levels. This finding may aid in breeding new drought-tolerant and high-yielding cotton hybrid varieties.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Darshan Chikkanayakanahalli Mukunda, Vijay Kumar Joshi, Subhash Chandra, Manjunath Siddaramaiah, Jackson Rodrigues, Shivaprasad Gadag, Usha Yogendra Nayak, Nirmal Mazumder, Kapaettu Satyamoorthy, Krishna Kishore Mahato
Summary: The study reports the suitability of deep-UV-LED as an excitation source for inducing autofluorescence in nonenzymatically glycated proteins. Deep-UV-induced autofluorescence of these proteins reveals structural and biochemical modifications, and can detect various clinically relevant advanced glycation end-products.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Xue Yi Tan, Azizah Misran, Leona Daniela Jeffery Daim, Benjamin Yii Chung Lau
Summary: Four methods were evaluated to extract proteins from Musang King durian pulps, with acetone-phenol method producing the best results. Differential protein analyses revealed that 15 proteins were down-regulated and 3 proteins were up-regulated after a year of frozen storage. This study indicates that frozen storage affects protein expression in various functional aspects.
Article
Chemistry, Analytical
Mojgan Sadat Shushtarian, Mohammad Hadi Givianrad, Mohammad Saber Tehrani, Parviz Aberoomand-Azar
Summary: This study developed an efficient strategy utilizing ion imprinted polymer (IIP) based mu-TLC-LA-ICP-MS for speciation analysis of inorganic arsenic. The IIP was characterized using FTIR and FESEM, and a TLC plate was successfully prepared. Under optimized conditions, the method demonstrated a low detection limit and wide linear dynamic range, with good performance in wastewater and human blood plasma samples.
ANALYTICAL METHODS
(2023)
Review
Biochemistry & Molecular Biology
Annunziata Lapolla, Pietro Traldi
Summary: Gestational diabetes mellitus (GDM) leads to complications for both mothers and infants, and is associated with changes in placenta structure and function. Various mass spectrometric approaches can be used to study the protein profile of placenta. These techniques have shown differences in protein expression between healthy and GDM placenta samples.
Article
Polymer Science
Hiroyuki Ohshima
Summary: This study develops a general theory for the time-dependent transient electrophoresis of a weakly charged spherical colloidal particle in a polymer gel medium. The Laplace transform of the transient electrophoretic mobility is derived by considering the hydrodynamic interaction between the particle and the gel medium based on the Brinkman-Debye-Bueche model. The theory covers both transient gel electrophoresis and transient free-solution electrophoresis, with the relaxation time for the former being shorter and decreasing as the Brinkman screening length decreases.
Article
Biochemistry & Molecular Biology
Deepesh D. Gaikwad, Nilima S. Bangar, Mayura M. Apte, Armaan Gvalani, Rashmi S. Tupe
Summary: This study discovers that the interaction between aldosterone and glycated albumin in diabetic nephropathy can cause oxidative stress, reduce levels of antioxidants and glycation detoxifying enzymes, and result in renal cell damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Applied
Weiyi He, Shuiming Li, Kan He, Fan Sun, Lixia Mu, Qingrong Li, Jiang Yi, Zhendan He, Zhigang Liu, Xuli Wu
Summary: The silkworm and its metabolites are important sources of allergens that can induce severe allergic reactions. This study identified 45 potential allergens in silkworm and found that they may cross-react with allergens from other insects and fungi. It is crucial to study these potential allergens for the prevention, diagnosis, and treatment of silkworm allergy.
Article
Parasitology
Guanggang Qu, Zhiyang Xu, Wenbin Tuo, Charles Li, Hyun Lillehoj, Gen Wan, Haiwei Gong, Jinxin Huang, Guichuan Tian, Shanxin Li, Yuxin Liu, Liheng Liu
Summary: In this study, the whole sporozoite proteins of Eimeria necatrix were analyzed using two-dimensional electrophoresis and Western blotting. Multiple unique proteins were identified and their structures, functions, and locations were analyzed. These findings contribute to our understanding of the parasite's immunogenicity and immune evasion mechanisms, and they also facilitate the discovery of effective vaccine candidates.
VETERINARY PARASITOLOGY
(2022)
Review
Chemistry, Analytical
M. Sejalon-Cipolla, P. Bruyat, S. Bregant, C. Malgorn, L. Devel, G. Subra, S. Cantel
Summary: MALDI-MS is crucial for detecting and identifying molecules involved in biological pathways, but many biomarkers remain undetectable due to various limitations. To overcome these challenges, labeling strategies and activity-based probes have been developed. Matrix-free derivatization techniques are currently gaining attention.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Holly-May Lewis, Priyanka Gupta, Kyle D. G. Saunders, Shazneil Briones, Johanna von Gerichten, Paul A. Townsend, Eirini Velliou, Dany J. V. Beste, Olivier Cexus, Roger Webb, Melanie J. Bailey
Summary: This work presents a new approach for measuring drug levels and lipid fingerprints in individual living mammalian cells. The method combines nanocapillary sampling with liquid chromatography to improve precision in drug analysis. By optimizing the transfer of analytes from the sampling capillary, the researchers achieved efficient measurement of drug analytes in 30 living cells. They also discovered cell-to-cell heterogeneity in drug molecule uptake and identified lipid features associated with bedaquiline uptake.
Review
Spectroscopy
Beatrice Zoanni, Maura Brioschi, Alice Mallia, Erica Gianazza, Sonia Eligini, Marina Carini, Giancarlo Aldini, Cristina Banfi
Summary: This review summarizes the important roles of human serum albumin (HSA) in cardiovascular diseases (CVD) and heart failure, emphasizing the physiological functions of highly abundant plasma proteins should not be neglected.
MASS SPECTROMETRY REVIEWS
(2023)
Review
Spectroscopy
Erica Gianazza, Beatrice Zoanni, Alice Mallia, Maura Brioschi, Gualtiero Colombo, Cristina Banfi
Summary: This narrative review highlights the potential of lipoprotein-associated proteins in cardiovascular risk assessment and emphasizes the importance of high-throughput proteomic analysis.
MASS SPECTROMETRY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Cristina Banfi, Patrizia Amadio, Marta Zara, Maura Brioschi, Leonardo Sandrini, Silvia S. Barbieri
Summary: The absence of PCYOX1 leads to platelet hypo-reactivity and impaired arterial thrombosis, suggesting PCYOX1 as a potential novel target for antithrombotic drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sonia Eligini, Benedetta Porro, Jose Pablo Werba, Nicolo Capra, Stefano Genovese, Arianna Greco, Viviana Cavalca, Cristina Banfi
Summary: The effects of oral glucose tolerance test (OGTT) on red blood cells (RBCs), particularly oxidative stress and L-arginine/Nitric Oxide (L-Arg/NO) metabolic pathway, were evaluated in patients with prediabetes. The results showed an increase in oxidative stress and a reduction in NO production in RBCs after glucose loading, suggesting the importance of avoiding acute glycemic variations to prevent diabetic complications, even in the early stages of diabetes.
Review
Biochemistry & Molecular Biology
Sonia Eligini, Erica Gianazza, Alice Mallia, Stefania Ghilardi, Cristina Banfi
Summary: Macrophages are versatile cells that can adapt their characteristics and functions to different environments. They play important roles in maintaining homeostasis and are also involved in various human diseases, including atherosclerosis. Proteomics has been widely used to study macrophages, and analyzing the proteome of different macrophage phenotypes can provide insights into their roles in atherosclerosis and identify potential therapeutic targets. In this review, we discuss different proteomic techniques used to profile macrophage phenotypes and summarize findings that contribute to understanding macrophage functions and developing strategies to combat atherosclerosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Marcos Leal Brioschi, Carlos Dalmaso Neto, Marcos de Toledo, Eduardo Borba Neves, Jose Viriato Coelho Vargas, Manoel Jacobsen Teixeira
Summary: This study proposed an infrared image-based method for febrile and subfebrile people screening to comply with the society need for alternative, quick response, and effective methods for COVID-19 contagious people screening.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Imaging Science & Photographic Technology
Flavio Leme Ferrari, Marcos Leal Brioschi, Carlos Dalmaso Neto, Carlos Roberto de Medeiros
Summary: The authors developed a new non-invasive dermatological infrared macroimaging analysis technique (MacroIR) that evaluates microvascular, inflammatory, and metabolic changes in skin and mucosal lesions. The technique combines naked eye observation, polarized light dermatoscopy (PLD), and MacroIR to compare results. Ten cases were evaluated using a smartphone coupled with a dermatoscope and a macro lens integrated far-infrared transducer into specific software. MacroIR imaging showed promise in improving dermatological diseases diagnosis.
JOURNAL OF IMAGING
(2023)
Review
Biochemistry & Molecular Biology
Erica Gianazza, Maura Brioschi, Ada Iezzi, Giuseppe Paglia, Cristina Banfi
Summary: Lipid-lowering therapies are widely used to prevent ASCVD and related mortality. Omics technologies have been employed to study the mechanisms and effects of these drugs, aiming to improve treatment efficacy and safety. Pharmacometabolomics focuses on studying the effects of drugs on metabolic pathways and the variation of treatment response. This review summarizes significant metabolomic studies on lipid-lowering therapies, including statins, fibrates, and novel drugs and nutraceutical approaches. Integrating pharmacometabolomics data with other omics approaches can help understand the biological mechanisms of lipid-lowering drugs and improve treatment efficacy while reducing side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cristina Banfi, Alice Mallia, Stefania Ghilardi, Maura Brioschi, Erica Gianazza, Sonia Eligini, Pelin Sahlen, Roberta Baetta
Summary: PCYOX1 is a regulator of adipogenesis, acting through multiple key factors. Its absence reduces adipogenesis and inflammatory markers, making it a potential adipogenic factor with therapeutic potential.
Article
Biochemistry & Molecular Biology
Maura Brioschi, Yuri D'Alessandra, Massimo Mapelli, Irene Mattavelli, Elisabetta Salvioni, Sonia Eligini, Alice Mallia, Veronica Ricci, Erica Gianazza, Stefania Ghilardi, Piergiuseppe Agostoni, Cristina Banfi
Summary: Sacubitril/Valsartan, a combination drug for heart failure treatment, reduces the levels of miR-29b-3p, miR-221-3p, and miR-503-5p in the plasma of patients with stable HF. These miRNAs have potential roles in HFrEF pathophysiology.
Article
Biochemistry & Molecular Biology
Marta Zara, Andrea Baggiano, Patrizia Amadio, Jeness Campodonico, Sebastiano Gili, Andrea Annoni, Gianluca De Dona, Maria Ludovica Carerj, Francesco Cilia, Alberto Formenti, Laura Fusini, Cristina Banfi, Paola Gripari, Calogero Claudio Tedesco, Maria Elisabetta Mancini, Mattia Chiesa, Riccardo Maragna, Francesca Marchetti, Marco Penso, Luigi Tassetti, Alessandra Volpe, Alice Bonomi, Giancarlo Marenzi, Gianluca Pontone, Silvia Stella Barbieri
Summary: This study found that the expression of CD41-CD61 in sEVs can reflect the CMR-assessed ischemic damage after STEMI, providing a new strategy for the timely identification of high-risk patients and their treatment optimization.
Article
Medicine, General & Internal
Marcelo Pastor Almada Davalos, Marcos Leal Brioschi, Samir Ezequiel da Rosa, Gabriel Carneiro Brioschi, Eduardo Borba Neves
Summary: This study explored the potential of dual infrared-visual thermography (IRVT) to identify perforator veins (PV) in asymptomatic veins. The results showed that IRVT effectively distinguishes varying degrees of vascular reflux severity. IRVT shows promise as a non-invasive, radiation-free tool to enhance PV identification.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Bruno Veloso Fracasso, Renato Bender Castro, Marcos Leal Brioschi, Tais Malysz
Summary: This study used infrared point-of-care thermography to analyze the facial temperature distribution of 24 female participants with chronic migraine. The findings revealed significant temperature asymmetry in women with right-sided unilateral headaches, particularly in the right frontal and temporal regions. However, caution is advised in interpreting these results, as further research with larger samples is needed.
JOURNAL OF CLINICAL MEDICINE
(2023)
Proceedings Paper
Computer Science, Artificial Intelligence
Denise Sabbagh Haddad, Marcos Leal Brioschi, Maria Aparecida Borsatti Luchetti, Nicolas Civiero, Mayco Anderson Moreira, Emiko Saito Arita
Summary: Inflammatory toothache is a common and debilitating condition that affects patients' quality of life and overall health. Infrared thermography, combined with artificial neural networks, can assist in the detection and accurate diagnosis of inflammatory toothache.
ARTIFICIAL INTELLIGENCE OVER INFRARED IMAGES FOR MEDICAL APPLICATIONS AND MEDICAL IMAGE ASSISTED BIOMARKER DISCOVERY
(2022)
