Article
Biotechnology & Applied Microbiology
Behrouz Golichenari, Shamsi Yari, Alireza Hadizadeh Tasbiti, Javad Behravan, Farzam Vaziri, Kiarash Ghazvini
Summary: In this study, we demonstrated the successful transfer of gene cassettes from Mycobacterium smegmatis to Mycobacterium tuberculosis, along with the confirmation of α1,3GT enzyme in the transconjugants.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Infectious Diseases
Svetlana G. Frolova, Ksenia M. Klimina, Ravinder Kumar, Aleksey A. Vatlin, Deepak B. Salunke, Pravin Kendrekar, Valery N. Danilenko, Dmitry A. Maslov
Summary: Tryptanthrin, a natural alkaloid, shows great therapeutic potential and resistance provided by the MmpS5-MmpL5 efflux system. Spontaneous tryptanthrin-resistant mutants of M. smegmatis exhibit mutations in genes such as MSMEG_1963 and MSMEG_5597, shedding light on the mechanisms of resistance to tryptanthrins.
Article
Microbiology
Oksana Bychenko, Yulia Skvortsova, Rustam Ziganshin, Artem Grigorov, Leonid Aseev, Albina Ostrik, Arseny Kaprelyants, Elena G. Salina, Tatyana Azhikina
Summary: The expression of MTS1338 in non-pathogenic Mycobacterium smegmatis slows down their growth rate and increases their survival in macrophages. MTS1338 also affects the expression of proteins, the synthesis of cell wall components, and the regulation of iron metabolism and hypoxia response.
Article
Nanoscience & Nanotechnology
Han Wang, Gloria M. Conover, Song- Han, James C. Sacchettini, Arum Han
Summary: A microfluidic mycobacterial culture device was developed for single-cell resolution analysis, successfully monitoring real-time growth dynamics of mycobacteria, revealing morphological changes during drug treatment and a subpopulation of cells capable of surviving frontline antibiotics and recovering replicative growth.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Ying Zhou, Tianying Zhong, Wenjing Wei, Zhuhua Wu, Anping Yang, Ning Liu, Ming Wang, Xiaoli Zhang
Summary: The study characterized the impact of downregulating Mtsp17 on gene expression in Myobacterium smegmatis, revealing that Mtsp17 may act as an activator of desA1 and regulate the SigF regulon through the SigF regulatory pathway. This sheds light on the transcriptional regulation role of Mtsp17 and provides new insights into the molecular mechanisms of START family proteins.
Article
Biochemistry & Molecular Biology
Romain Veyron-Churlet, Sophie Lecher, Anne-Sophie Lacoste, Jean-Michel Saliou, Camille Locht
Summary: Cholesterol is essential for the virulence of Mycobacterium tuberculosis and can be used as a carbon source. Identifying the molecular partners involved in cholesterol catabolism is important for developing new antitubercular drugs. A proximity-based biotin identification approach was used to study protein-protein interactions and metabolic channeling in mycobacteria.
Review
Immunology
Nan Wang, Yongliang Yao, Yingfen Qian, Dewen Qiu, Hui Cao, Huayuan Xiang, Jianjun Wang
Summary: Exosomes are double-membrane vesicles containing various lipids, proteins, mRNAs, and non-coding RNAs, and are involved in multiple physiological processes such as intercellular communication and immunomodulation. Studies have found that the components of exosomes released from host cells are altered following Mycobacterium tuberculosis infection. The contents of exosomes provide excellent biomarkers for the diagnosis, efficacy evaluation, and prognosis of tuberculosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Huanhuan Ning, Xuan Liang, Yanling Xie, Lu Bai, Wei Zhang, Lifei Wang, Jian Kang, Yanzhi Lu, Yanling Ma, Guangchun Bai, Yinlan Bai
Summary: This study investigates the role of c-di-AMP in regulating mycobacterial physiology and immunogenicity. Elevated c-di-AMP levels in Mycobacterium smegmatis were found to result in smaller colonies, shorter bacteria length, impaired growth, and inhibition of potassium transporter. The study also discovered that elevated c-di-AMP levels can inhibit biofilm formation and induce porphyrin accumulation, which may affect drug resistance and virulence of mycobacterium.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Chemistry, Physical
Shiva Mohammadi-Jam, Kristian E. Waters, Richard W. Greenwood
Summary: This article reviews the use of the electroacoustic technique in determining the zeta potential of concentrated aqueous suspensions and emphasizes the importance of identifying the isoelectric points for colloidal stability and processing. The application of the technique in polymer lattices is also summarized.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Multidisciplinary Sciences
Nicole C. Cardoso, Andrea O. Papadopoulos, Bavesh D. Kana
Summary: The study identified the functional roles of different nitrate reductase genes in Mycobacterium smegmatis, with only MSMEG_4206 playing a significant role in nitrate assimilation.
Review
Immunology
Weile Xie, Longlong Wang, Dan Luo, Vijay Soni, Eric H. Rosenn, Zhe Wang
Summary: Mycobacterium smegmatis (M.sm) is a widely used alternative model organism for studying Mycobacterium tuberculosis (M.tb). It has high sequence homology with M.tb but is considered non-pathogenic in humans. Recent advancements in vaccinomics tools that support the efficacy of a M.sm-based vaccine vector are reviewed in this article, highlighting the potential for developing next-generation recombinant vaccines against rapidly developing diseases through the integration of systems biology and molecular omics techniques.
Article
Multidisciplinary Sciences
Aditya Kumar Pal, Anirban Ghosh
Summary: This study investigates the role of secondary messenger c-di-AMP in drug tolerance, specifically in Mycobacterium smegmatis. The findings highlight the specific molecular mechanism linking elevated c-di-AMP levels with resistance mutagenesis and emphasize the significance of non-homology-based DNA repair. Additionally, the study identifies the unique mutational landscape associated with intracellular c-di-AMP levels and demonstrates the critical role of c-di-AMP in driving the evolution of multi-drug tolerance.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Zhi Zhang, Yao Wang, Qun Chen, Qingguo Gao, Liming Liu, Jianjun Yang, Xinjian Pan, Yu Miao, Feng Chi
Summary: This paper presents the preparation of novel blue electrophoretic particles using copper (II) phthalocyanine and a high ionization mono ionic liquid. The modified particles showed improved dispersion stability and reversible response in an electrophoretic display (EPD) cell. The results suggest that the use of high ionization mono ionic liquid has great applicability for modifying electrophoretic particles, making them excellent candidates for EPDs.
Article
Immunology
Heather A. Parker, Nina Dickerhof, Lorna Forrester, Heath Ryburn, Leon Smyth, Joris Messens, Htin L. Aung, Gregory M Cook, Anthony J. Kettle, Mark B. Hampton
Summary: The study found that M. smegmatis can resist the HOCl produced inside neutrophil phagosomes, and the main reason may not be its main low-molecular-weight thiol. These findings provide new opportunities for research on how to enhance the destruction of pathogenic mycobacteria by the immune system.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Geosciences, Multidisciplinary
Mutlaq Alarouj, Harry Collini, Matthew D. Jackson
Summary: The zeta potential in natural sandstones is typically negative, but in this study, sandstone samples containing dolomite and siderite showed positive zeta potentials. This unexpected result was attributed to the adsorption of divalent ions onto mineral surfaces and ion exchange processes. The findings emphasize the importance of realistic experimental conditions in measuring zeta potential and highlight the impact of zeta potential on geophysical monitoring and contaminant transport in sandstone aquifers.
GEOPHYSICAL RESEARCH LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Lorena Novoa-Aponte, David Ramirez, Jose M. Arguello
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Physiology
Rita Rosenthal, Dorothee Guenzel, Joerg Piontek, Susanne M. Krug, Carlos Ayala-Torres, Caroline Hempel, Dian Theune, Michael Fromm
Review
Parasitology
Bibiana Chavarro-Portillo, Carlos Yesid Soto, Martha Inirida Guerrero
Article
Biochemistry & Molecular Biology
Carlos Ayala-Torres, Susanne M. Krug, Joerg D. Schulzke, Rita Rosenthal, Michael Fromm
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Paola Santos, Fabian Lopez-Vallejo, David Ramirez, Julio Caballero, Dulce Mata Espinosa, Rogelio Hernandez-Pando, Carlos Y. Soto
BIOORGANIC & MEDICINAL CHEMISTRY
(2020)
Article
Agriculture, Dairy & Animal Science
Nelson E. Arenas, Edier F. Avila, Edwin D. Correa, Wendy N. Rueda, Gina M. Lopez, Carlos Y. Soto
Summary: An epidemiological tool was designed to monitor bovine tuberculosis (BTB) and brucellosis in Colombia. Analysis showed provinces of Cundinamarea and Valle del Cauca were affected by BTB and brucellosis epidemics, with increased case detection in 2012 and 2019 respectively. This platform allows tracking hotspots, identifying trends, and providing useful information for control programs.
REVISTA COLOMBIANA DE CIENCIAS PECUARIAS
(2021)
Article
Biochemistry & Molecular Biology
Carlos Ayala-Torres, Susanne M. Krug, Rita Rosenthal, Michael Fromm
Summary: Water transport in epithelia is regulated by tight junction proteins, with modulation of these proteins affecting ion and macromolecule permeability in tight and intermediate-tight epithelial cell lines. However, transepithelial water permeability is only affected in tight cell lines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Milena Maya-Hoyos, Dulce Mata-Espinosa, Manuel O. Lopez-Torres, Blanca Tovar-Vazquez, Jorge Barrios-Payan, Juan C. Leon-Contreras, Marisol Ocampo, Rogelio Hernandez-Pando, Carlos Y. Soto
Summary: Identification and targeting of CtpF as a potential attenuation target for Mycobacterium tuberculosis (Mtb) has shown reduced proliferation and virulence in mouse models, while maintaining immunogenicity. Differential expression levels of ctpF in latent and progressive TB mouse models suggest its involvement in Mtb persistence. Possible complementary mechanisms for Ca2+ transport deficiencies mediated by P-type ATPases are also indicated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Bibiana Chavarro-Portillo, Carlos Y. Soto, Martha Inirida Guerrero
Summary: This study assessed the infectivity in Schwann cells (SCs) of recurrent and non-recurrent Mycobacterium leprae strains and their correlation with genes involved in phenolic glycolipid I (PGL-I) biosynthesis. The initial infectivity of non-recurrent strains in SCs was higher than a recurrent strain, and both strains showed increased infectivity as the trials progressed. However, non-recurrent strains displayed the maximum infectivity at 12 days post-infection. Furthermore, qRT-PCR experiments revealed that key genes involved in PGL-I biosynthesis were transcribed faster in non-recurrent strains compared to the recurrent strain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Alejandro Sierra, Danna Camelo, Camila Lota, Nelson Enrique Arenas, Carlos Y. Soto
Summary: In this study, a Loop-Mediated Isothermal Amplification (LAMP-PCR) targeting the Region of Difference 12 (RD12) of the M. bovis genome was designed for the rapid and specific detection of M. bovis in small and medium-sized livestock. The LAMP method allowed for the positive identification of M. bovis within 30 minutes through a basic colorimetric reaction visible to the naked eye under natural light. The detection limit of the method was approximately 50 fg of M. bovis genomic DNA, equivalent to about 10 copies of the genome.
Review
Biochemistry & Molecular Biology
Lorena Novoa-Aponte, Jose M. Arguello
Summary: Copper distribution in cells is regulated by specific dynamic interactions and metal exchange, playing a vital role in maintaining cellular homeostasis.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
G. Astrid Garzon, Carlos Y. Soto, Marcela Lopez-R, Kenneth M. Riedl, Cindi R. Browmiller, Luke Howard
Article
Biology
Andres Leon-Torres, Epifania Arango, Eliana Castillo, Carlos Y. Soto
BIOLOGICAL RESEARCH
(2020)
Article
Multidisciplinary Sciences
Milena Maya-Hoyos, Cristian Rosales, Lorena Novoa-Aponte, Elianna Castillo, Carlos Y. Soto