Article
Environmental Sciences
Myriam Ben Said, Latifa Bousselmi, Ahmed Ghrabi
Summary: In this study, a rapid and reliable non-culture method for detecting and estimating bacteriophage titres was proposed. The method utilized titanium dioxide nanoparticles as nanosensors to detect and monitor the virus. By analyzing the dispersion and aggregation behavior of the nanoparticles in an aqueous solution, the researchers were able to determine the phage titre. The method was successfully applied to monitor the phage amplification cycle for detecting bacteria in a viable but non-culturable state after water treatment.
ENVIRONMENTAL TECHNOLOGY
(2023)
Article
Food Science & Technology
Xiaoming Yuan, Zhenjun Zhu, Zhichao Huang, Shan Yu, Hui Jin, Bo Chen, Shubo Yu, Liang Xue, Moutong Chen, Jumei Zhang, Juan Wang, Qingping Wu, Yu Ding
Summary: In this study, a simple and efficient method for engineering Bacillus cereus phages was developed to reduce residual phages and expand their host range. The strategy transformed phages from active viruses into more controllable antibacterial agents, making them safer and more efficient for the prevention and control of B. cereus.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
M. L. Domingo-Calap, M. Bernabeu-Gimeno, C. M. Aure, E. Marco-Noales, P. Domingo-Calap
Summary: Xanthomonas is a genus of plant-pathogenic bacteria that causes serious economic losses, and bacteriophages show promise as biocontrol tools to combat these pathogens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Katja Suster, Andrej Cor
Summary: Prosthetic joint infections are often caused by biofilm formation and the presence of viable but non-culturable bacteria. Conventional sample culturing is the standard for microbiological diagnosis, but it fails to detect non-culturable bacteria. This study investigated the use of bacteriophages to detect non-culturable bacteria.
Article
Engineering, Environmental
Maria Fiorella Jacob, Andrea del Lujan Quiberoni, Orlando Mario Alfano, Maria de los Milagros Ballari, Mariangeles Briggiler Marco
Summary: A photocatalytic paint formulated with commercial carbon-doped anatase TiO2 was used to test the inactivation of sixteen bacteriophages. The results showed that eight phages were completely inactivated within a short time (1.5-5 h), while the remaining eight phages partially reduced their infectivity over varying periods (4-20 h). The highest and lowest photonic inactivation efficiencies were 4.06 x 1012 PFU/Einstein and 3.00 x 109 PFU/ Einstein, respectively.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Immunology
Rocio Barron-Montenegro, Dacil Rivera, Maria Jesus Serrano, Rodrigo Garcia, Diana M. alvarez, Julio Benavides, Fernanda Arredondo, Francisca P. alvarez, Roberto Bastias, Soledad Ruiz, Christopher Hamilton-West, Eduardo Castro-Nallar, Andrea I. Moreno-Switt
Summary: Salmonella spp. is a worldwide foodborne pathogen, and bacteriophages represent an alternative to antimicrobials for mitigating Salmonella infections. In this study, Salmonella Enteritidis and a lytic phage were coevolved in a culture medium for 21 days. The results showed that both bacteria and phage could survive and develop resistance to each other during the coevolution process. The interactions between Salmonella and lytic phages are crucial for the rational use of phages to control this important foodborne pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Simone Eckstein, Jana Stender, Sonia Mzoughi, Kilian Vogele, Jana Kuehn, Daniela Friese, Christina Bugert, Susann Handrick, Mustapha Ferjani, Roman Woelfel, Andrew Millard, Mohamed Ben Moussa, Joachim J. Bugert
Summary: K64, K17, and K27 are the most common wzi capsule types in this geographical location in Northern Africa. The lytic phage TUN1 efficiently lyses K64 Kp strains associated with increased case/fatality rates at body temperature. With the ability to be rescued in a non-replicative host, the phage TUN1 enhances its utility as an antibacterial agent.
Article
Virology
Lujia Cen, Yunjie Chang, Joseph K. Bedree, Yansong Ma, Qiu Zhong, Daniel R. Utter, Pu-Ting Dong, Renate Lux, Batbileg Bor, Jun Liu, Jeffrey S. McLean, Shuai Le, Xuesong He
Summary: Bacteriophages are an important component of the human oral microbiome, with specific phages like LC001 showing remarkable host specificity targeting Schaalia odontolytica. The study suggests that LC001 may exploit the host bacterium-encoded lytic transglycosylase function to access the receptor, facilitating infection.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Nataliia Korniienko, Alla Kharina, Nikita Zrelovs, Barbora Jindrichova, Tomas Moravec, Iryna Budzanivska, Lenka Burketova, Tetiana Kalachova
Summary: In this study, two phages were isolated from market-bought pepper fruit and characterized. One of the phages was found to significantly inhibit the propagation of Pseudomonas syringae pv. tomato in plant tissues. This research contributes to the understanding of phage-host interactions in different plant systems and suggests the potential use of this phage as a biocontrol agent.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Robby Concha-Eloko, Pilar Barberan-Martinez, Rafael Sanjuan, Pilar Domingo-Calap
Summary: The emergence of antibiotic-resistant bacteria is a serious threat to global health, and new treatments are needed. This study presents three new Klebsiella phages with a broad host range, which hold potential for combating infections by multiple capsular types of Klebsiella pneumoniae.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Gregory S. Canfield, Anushila Chatterjee, Juliel Espinosa, Mihnea R. Mangalea, Emma K. Sheriff, Micah Keidan, Sara W. McBride, Bruce D. McCollister, Howard C. Hang, Breck A. Duerkop
Summary: Enterococcus faecium, a commensal of the human intestine, has evolved into a hospital-adapted, multidrug-resistant pathogen. Bacteriophages, natural predators of bacteria, show potential as therapeutics against MDR E. faecium infections, despite the unknown molecular events governing their interactions. While phage resistance may emerge, it is shown that lytic phages could effectively synergize with antibiotics to slow or inhibit E. faecium growth.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Liliam K. Harada, Erica C. Silva, Fernando P. N. Rossi, Basilio Cieza, Thais J. Oliveira, Carla Pereira, Geizecler Tomazetto, Bianca B. Silva, Fabio M. Squina, Marta M. D. C. Vila, Joao C. Setubal, Taekjip Ha, Aline M. da Silva, Victor M. Balcao
Summary: In this study, two lytic phages that infect multidrug-resistant P. aeruginosa strains were isolated and characterized. Increasing the multiplicity of infection (MOI) significantly improved phage efficiency and delayed bacterial regrowth. Neither of the phages carried toxin, virulence, antibiotic resistance, or integrase genes. These findings are highly relevant in the context of bacterial resistance to antibiotics.
FUTURE MICROBIOLOGY
(2022)
Article
Microbiology
Kai Wang, Dawei Chen, Quanrong Liu, Pengfei Zhu, Ming Sun, Donghai Peng
Summary: In this study, a novel lytic bacteriophage named vB_RsoP_BMB50 was isolated from tomato fields in Dalian, China. It belonged to the subfamily Okabevirinae, Autographiviridae family, and order Caudovirales. The phage exhibited a large burst size, high temperature stability, and strong pH tolerance. Comparative analyses revealed that vB_RsoP_BMB50 represented a novel Ralstonia phage genus.
CURRENT MICROBIOLOGY
(2022)
Article
Microbiology
Chao Feng, Kaixiang Jia, Teng Chi, Shuaimin Chen, Huabo Yu, Liang Zhang, Sayed Haidar Abbas Raza, Ahmed Mohajja Alshammari, Shuang Liang, Zishan Zhu, Tingxuan Li, Yanling Qi, Xiaofeng Shan, Aidong Qian, Dongxing Zhang, Lei Zhang, Wuwen Sun
Summary: Phage therapy using the bacteriophage PZL-Ah152 showed efficacy in eliminating pathogenic A. hydrophila in both in vivo and in vitro studies. The phage also exhibited no significant adverse effects in the treated fish and had minimal impact on the gut microbiota.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Mariangeles Briggiler Marco, Maria de los Milagro Ballari, Jesica Soledad Gornati, Orlando Mario Alfano, Andrea Quiberoni
Summary: This study investigated the photocatalytic inactivation efficiencies of eight phages infective to lactic acid bacteria in bioaerosols using semi-pilot scale equipment. Complete inactivation of some phages was achieved within 100 minutes, while partial inactivation was observed for one phage under the same conditions. Additionally, efficiencies were calculated and the phage with the highest resistance may require further study for optimization of operation and efficiency.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)