Article
Chemistry, Analytical
Di Wu, Haotian Du, Xiaoshi Yan, Guifen Jie
Summary: In this study, a novel fluorescence biosensing platform based on carbon quantum dot (CQDs) quenching and enzyme-assisted multiple cycle amplification was developed for miRNA detection. The biosensor exhibited a wide linear range and low detection limit, showing great potential for early cancer diagnosis.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Yuanqing Xu, Weibiao Wang, Zhexue Chen, Xinyu Sui, Aocheng Wang, Cheng Liang, Jinquan Chang, Yanhong Ma, Luting Song, Wenyu Jiang, Jin Zhou, Xinfeng Liu, Yong Zhang
Summary: A general strategy has been developed to mechanically tailor semiconductor bulk materials into quantum dots, resulting in high yields of various semiconductor QDs. The produced QDs exhibit outstanding water solubility and remarkable photoluminescence, showing potential for practical applications in solid-state fluorescence and nonlinear saturation absorption.
Article
Chemistry, Multidisciplinary
Sung Hyun Kim, Hyunwoo Kim, Hawoong Jeong, Tae-Young Yoon
Summary: The study demonstrates the use of single-molecule FRET technology to encode virtual signals in DNA barcodes, allowing for precise measurement of FRET efficiency for each binding event and differentiation of six DNA barcodes.
Article
Chemistry, Analytical
Marketa Prochazkova, Eliska Kuchovska, Michael Killinger, Karel Kleparnik
Summary: In this study, a molecular probe based on Fo center dot rster resonance energy transfer (FRET) was designed and synthesized for investigating biological phenomena at a single-cell level. The probe utilizes a highly luminescent quantum dot (QD) as a donor and a specific peptide as a linker to a fluorophore or fluorescence quencher as an acceptor. The synthesized probe showed high reaction yield and specificity, and provided longer imaging times of caspases compared to commercial products.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Jack W. Shepherd, Sarah Lecinski, Jasmine Wragg, Sviatlana Shashkova, Chris MacDonald, Mark C. Leake
Summary: This study used cutting-edge genomically integrated FRET biosensing to determine localized molecular crowding in single live yeast cells, finding that crowding is reduced when yeast is grown in elevated glucose concentrations. Simulations indicated that the cell membrane is largely inaccessible to these sensors, and that cytosolic crowding is broadly uniform across each cell over a timescale of seconds.
Article
Chemistry, Multidisciplinary
Ajmeeta Sangtani, Okhil K. Nag, Eunkeu Oh, Michael H. Stewart, James B. Delehanty
Summary: In this study, a quantum dot-chlorin e6 nanobioconjugate system was developed for membrane-localized ROS generation, leading to lipid peroxidation, increased membrane permeability, and inhibition of cellular proliferation. This system represents a promising approach for the further evolution of QD-based PDT systems.
JOURNAL OF NANOPARTICLE RESEARCH
(2021)
Article
Chemistry, Physical
Ganapathi Bharathi, Devaraj Nataraj, Oleg Yu Khyzhun, Daniel T. Thangadurai, Kittusamy Senthilkumar, Murugaiyan Sowmiya, Ramasamy Kathiresan, Ponmalai Kolandaivel, Mukul Gupta, Deodatta Phase, Nirmalendu Patra, Shambhu Nath Jha, Dibyendu Bhattacharyya
Summary: A graphene system with opened energy bandgap and weak ferromagnetic behavior was prepared through a hydrothermal process, where graphene quantum dots (GQDs) were interconnected by iron (Fe) metal atoms. The interconnected solid sheets exhibited intrinsic optical absorption and photoluminescence properties, while retaining quantum behavior of the GQDs despite their micron size. The structural discontinuity around the metal atoms helped maintain the GQD behavior and the Fe atoms provided weak ferromagnetic behavior at room temperature.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Peng Yu, Kui Yan, Shangfeng Wang, Chenzhi Yao, Zuhai Lei, Yaohui Tang, Fan Zhang
Summary: Researchers have developed a fluorescent dyad in the NIR-II window, which shows improved spectral fidelity and serum tolerance for monitoring oxidative stress and evaluating therapeutic efficiency in traumatic brain injury.
Article
Chemistry, Physical
Augustin Caillas, Stephan Suffit, Pascal Filloux, Emmanuel Lhuillier, Aloyse Degiron
Summary: This study brings fresh insights into the ensemble properties of PbS colloidal quantum dots by conducting experiments to investigate the radiative recombination of thermalized photocarriers. A phenomenological model based on the Kirchhoff law is proposed to identify the nature of the thermalized photocarriers and extract their temperatures. Two thermalization regimes are observed: one involving photocarriers distributed over a wide energy range and the other solely involving fundamental excitons.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Review
Chemistry, Physical
Tanveer A. Tabish, Hasan Hayat, Aumber Abbas, Roger J. Narayan
Summary: The electrochemical biosensing systems coupled with graphene quantum dots have shown great potential for cancer diagnostic strategies, especially in identifying early tumorigenesis changes and detecting ultralow concentrations of biomarkers for distinguishing normal and malignant cells. Graphene quantum dots, with their comparable size to biomolecules, provide an ideal platform for studying proteins, cells, and viruses.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Xinmei Liu, Farsad Imtiaz Chowdhury, Lingju Meng, Qiwei Xu, Xihua Wang
Summary: This study introduces a method for preparing high-quality luminescent films using QD-CNC hybrid materials, which involves replacing the organic ligands on the QD surface and acetylating the CNC surface to overcome the challenge of dissolving QD and environmentally friendly material CNC simultaneously. The fabricated film maintains similar photoluminescence quantum yield and lifetime to QDs synthesized in non-polar organic solvents.
Article
Nanoscience & Nanotechnology
Hua Chai, Wenbo Cheng, Dayong Jin, Peng Miao
Summary: This article summarizes the assembly principles and applications of hybridization chain reaction (HCR) in DNA nanotechnology. HCR has excellent assembly kinetics and ease of operation, making it suitable for ultra-sensitive detection of various targets. Future advancements in overcoming challenges are needed for further development in this field.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Chemistry, Multidisciplinary
Niko Hildebrandt, Mihye Lim, Namjun Kim, Da Yeon Choi, Jwa-Min Nam
Summary: Plasmonic metal nanoparticles and semiconductor quantum dots are widely used nanomaterials in optical biosensing and bioimaging. While their combination for fluorescence quenching via nanosurface energy transfer or Forster resonance energy transfer is common, metal-QD nanohybrids for plasmon-enhanced fluorescence have been less prevalent. This article discusses the challenges in optimizing distances, orientations, and spectral overlap for maximum plasmon-enhanced fluorescence and reviews the different nanomaterials, their combinations, and applications in advanced optical biosensing and bioimaging.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Jinhyeon Kang, Jae Taek Oh, Hyeokjun Kim, Hanjae Kim, Hyosung Choi, In Hwan Jung
Summary: This study reports the development of high-performance AgBiS2 nanocrystal-based quantum-dot photodetectors (QPDs) with improved photodetection ability. A novel conjugated polyelectrolyte (CPE) was incorporated as a cathode buffer layer (CBL) to enhance the photocurrent density and reduce the dark current density.
APPLIED SURFACE SCIENCE
(2022)
Article
Spectroscopy
Adity Chopra, Anuradha Swami, Rohit Sharma, Neha Devi, Sherry Mittal, Rohit K. Sharma, Nishima Wangoo
Summary: This study presents a fluorescence resonance energy transfer-based assay for the detection of Staphylococcal enterotoxin. The developed method showed high sensitivity and specificity, and was successfully applied for quantification in apple juice.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Engineering, Biomedical
Manon Debayle, Elie Balloul, Fatimata Dembele, Xiangzhen Xu, Mohamed Hanafi, Francois Ribot, Cornelia Monzel, Mathieu Coppey, Alexandra Fragola, Maxime Dahan, Thomas Pons, Nicolas Lequeux
Article
Chemistry, Multidisciplinary
Subha Jana, Xiangzhen Xu, Andrey Klymchenko, Andreas Reisch, Thomas Pons
Summary: The Whispering gallery mode (WGM) microcavities are potential candidates in biosensing applications as their resonance wavelengths shift with changes in the refractive index. They offer high-quality resonance modes and accessible surface functionalities, making them promising for molecular assays, but their high sensitivity also makes them inherently unstable. The WGM resonances are also shown to strongly enhance fluorescence energy transfer between donors and acceptors, providing a potentially powerful class of biosensors combining sensitivity and simple detection schemes.
Article
Chemistry, Multidisciplinary
Nicolas Moghaddam, Corentin Dabard, Marion Dufour, Hong Po, Xiangzhen Xu, Thomas Pons, Emmanuel Lhuillier, Sandrine Ithurria
Summary: The study demonstrates a method to increase the thickness of two-dimensional II-VI semiconductor nanoplatelets by replacing native carboxylate ligands with halides. Through a dissolution/recrystallization process, the thickness can be increased to 9 ML, while also achieving the growth of CdSe/CdSe, CdTe/CdTe, and CdSe/CdTe core/shell homo- and heterostructures. This method affords a new degree of freedom for the growth of structured nanoplatelets with designed band engineering, expanding the capabilities beyond what has been achievable for heteromaterial nanostructures.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Physics, Multidisciplinary
Victor Blondot, Alexandra Bogicevic, Antoine Coste, Christophe Arnold, Stephanie Buil, Xavier Quelin, Thomas Pons, Nicolas Lequeux, Jean-Pierre Hermier
NEW JOURNAL OF PHYSICS
(2020)
Article
Oncology
Ilya Yakavets, Aurelie Francois, Maelle Guiot, Nicolas Lequeux, Alexandra Fragola, Thomas Pons, Lina Bezdetnaya, Frederic Marchal
Correction
Multidisciplinary Sciences
Alessandra Stangherlin, Joseph L. Watson, David C. S. Wong, Silvia Barbiero, Aiwei Zeng, Estere Seinkmane, Sew Peak Chew, Andrew D. Beale, Edward A. Hayter, Alina Guna, Alison J. Inglis, Marrit Putker, Eline Bartolami, Stefan Matile, Nicolas Lequeux, Thomas Pons, Jason Day, Gerben van Ooijen, Rebecca M. Voorhees, David A. Bechtold, Emmanuel Derivery, Rachel S. Edgar, Peter Newham, John S. O'Neill
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Alessandra Stangherlin, Joseph L. Watson, David C. S. Wong, Silvia Barbiero, Aiwei Zeng, Estere Seinkmane, Sew Peak Chew, Andrew D. Beale, Edward A. Hayter, Alina Guna, Alison J. Inglis, Marrit Putker, Eline Bartolami, Stefan Matile, Nicolas Lequeux, Thomas Pons, Jason Day, Gerben van Ooijen, Rebecca M. Voorhees, David A. Bechtold, Emmanuel Derivery, Rachel S. Edgar, Peter Newham, John S. O'Neill
Summary: This study reveals that circadian control of the proteome impacts ion homeostasis in cells, especially cardiomyocytes, through mechanisms involving osmotic compensation via electroneutral ion transport. Changes in ion content due to perturbation of soluble protein abundance drive daily rhythms in cardiomyocyte electrical activity, with substantial consequences for cell physiology.
NATURE COMMUNICATIONS
(2021)
Review
Oncology
Fanny Delille, Yuzhou Pu, Nicolas Lequeux, Thomas Pons
Summary: Inorganic nanocrystals, such as gold, iron oxide, and semiconductor quantum dots, have specific optical or magnetic properties that are promising for cancer diagnostics, imaging, and therapy. The surface ligands of these nanoparticles are crucial for their colloidal stability in biological fluids and enable functionalization for targeting purposes. Nanoparticle surface chemistry plays a critical role in determining biodistribution and overall imaging or therapeutic efficiency, with ongoing studies focusing on developing optimal strategies for in vivo applications.
Article
Chemistry, Multidisciplinary
Zijun Wang, Fanny Delille, Sophie Bartier, Thomas Pons, Nicolas Lequeux, Bruno Louis, Jongwook Kim, Thierry Gacoin
Summary: Dynamics with orientational freedom are fundamental in biological events. Lanthanide-doped nanocrystals with polarized luminescence provide precise analysis and are more stable than quantum dots. However, the polarization property often deteriorates in complex physiological environments. In this study, we demonstrate a surface functionalization method for lanthanide-doped nanorods using zwitterionic block copolymers. The coated nanorods exhibit high colloidal stability, preserved shear-aligned ability, strongly polarized emission, and good biocompatibility. This study validates the strategy of coupling zwitterions to lanthanide-doped nanocrystals for various bioapplications.
Review
Pharmacology & Pharmacy
Luca Dirheimer, Thomas Pons, Frederic Marchal, Lina Bezdetnaya
Summary: Quantum dots (QDs) are fluorescent nanoparticles with exceptional optical properties, making them a promising choice for bioimaging applications. However, the challenge lies in their ability to specifically label targeted cells. In addition to studies conducted in two-dimensional cell monolayers, recent research has also focused on using three-dimensional multicellular tumor models for a better understanding of QDs behavior.
Article
Optics
V Blondot, C. Arnold, A. Delteil, D. Gerard, A. Bogicevic, T. Pons, N. Lequeux, J. P. Hugonin, J. J. Greffet, S. Buil, J. P. Hermier
Summary: We report the synthesis of hybrid light emitting particles with a diameter ranging from 100 to 500 nm, consisting of a compact CdSe/CdS/CdZnS semiconductor nanocrystal aggregate encapsulated by controlled nanometric size silica and gold layers. The addition of the gold nanoshell enhances the Purcell decay rate, as predicted by numerical simulations. Furthermore, we demonstrate the inhibition of Forster resonance energy transfer contribution.
Article
Nanoscience & Nanotechnology
Paul Robineau, Jeremie Beal, Thomas Pons, Rodolphe Jaffiol, Cyrille Vezy
Summary: Micron-scale patterning of colloidal quantum dots is crucial for various applications. A new method is proposed to create micropatterns of QDs with high resolution using UV photolithography and silanization. The ability to bind proteins on the QDs surface opens up possibilities for biofunctionalization and nanoimaging.
ACS APPLIED NANO MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Yuzhou Pu, Thomas Pons
Summary: Gold nanoparticles, particularly nanorods (AuNRs), have high absorption cross sections in the near-infrared (NIR) range, making them valuable as photosensitizers for cancer treatment. By combining AuNRs with TiO2, the hybrid nanoparticles can generate hydroxyl radicals under NIR irradiation. The photodynamic effect induced by these nanoparticles is more efficient in killing cancer cells than the photothermal effect alone.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Fanny Delille, Elie Balloul, Bassam Hajj, Mohamed Hanafi, Colin Morand, Xiang Zhen Xu, Simon Dumas, Antoine Coulon, Nicolas Lequeux, Thomas Pons
Summary: Exerting forces on biomolecules inside living cells by using magnetic iron oxide nanoparticles as a tool shows promise for probing their dynamic interactions in their natural environment. However, the nanoparticles' use has been limited to extracellular biomolecules. Here, we introduce a new type of ligand for magnetic nanoparticles that allows efficient targeting in the nucleus of living cells, enabling magnetic manipulation of specific genomic loci. These stable and sensitive magnetic nanoprobes have the potential to be a valuable tool for manipulating specific biomolecules and probing the mechanical properties of living matter at the molecular scale.
Meeting Abstract
Chemistry, Multidisciplinary
Manon Debayle, Elie Balloul, Fatima Dembele, Xiangzhen Xu, Mohamed Hanafi, Cornelia Monzel, Mathieu Coppey, Maxime Dahan, Alexandra Fragola, Thomas Pons, Nicolas Lequeux
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
