Article
Biochemistry & Molecular Biology
Jingwen Feng, Dongyang Li, Jin Zhang, Xiushan Yin, Jian Li
Summary: The 3C-like protease (M-pro, 3CL(pro)) plays a critical role in the replication process of coronaviruses. Baicalein has been found to exhibit specific activity against SARS-CoV M-pro, making it a potential candidate for the development of antiviral drugs. This study provides a structural basis for the research and development of baicalein as an anti-CoVs drug.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Hao Wang, Rongjuan Pei, Xin Li, Weilong Deng, Shuai Xing, Yanan Zhang, Chen Zhang, Shuai He, Hao Sun, Shuqi Xiao, Jin Xiong, Yecheng Zhang, Xinwen Chen, Yaxin Wang, Yu Guo, Bo Zhang, Luqing Shang
Summary: COVID-19 caused by SARS-CoV-2 has resulted in millions of infections and deaths worldwide. Researchers have identified the 3CL protease of SARS-CoV-2 as a potential target for developing drugs. They have successfully designed peptidomimetic inhibitors with excellent antiviral activity against SARS-CoV-2, indicating their potential as drug candidates.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Public, Environmental & Occupational Health
Carolina De Marco Verissimo, Jesus Lopez Corrales, Amber L. Dorey, Krystyna Cwiklinski, Richard Lalor, Nichola Eliza Davies Calvani, Heather L. Jewhurst, Andrew Flaus, Sean Doyle, John P. Dalton
Summary: This study describes the expression of a highly soluble and functionally active recombinant 3CL-pro and investigates its inhibitory profile and ability to function as a carrier for the receptor binding domain. The findings suggest that chimeric proteins containing 3CL-pro may be a potential approach to developing new COVID-19 vaccines.
EPIDEMIOLOGY AND INFECTION
(2022)
Article
Biochemistry & Molecular Biology
Wei-Chung Chiou, Meng-Shiuan Hsu, Yun-Ti Chen, Jinn-Moon Yang, Yeou-Guang Tsay, Hsiu-Chen Huang, Cheng Huang
Summary: Researchers have identified several FDA-approved drugs that can effectively inhibit the proteolytic activity of SARS-CoV-2 virus, demonstrating their therapeutic potential for treating COVID-19 and other Betacoronavirus infections.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Wei-Chung Chiou, Jui-Chieh Chen, Yun-Ti Chen, Jinn-Moon Yang, Lih-Hwa Hwang, Yi-Shuan Lyu, Hsin-Yi Yang, Cheng Huang
Summary: The COVID-19 pandemic has caused a global health crisis, and developing effective treatments is crucial. This study investigates the role of SARS-CoV-2 3CLpro in viral replication and identifies natural compounds PGG and EGCG as potential inhibitors. The findings highlight the therapeutic potentials of PGG and EGCG for treating SARS-CoV-2 infection.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Wei-Chung Chiou, Hsu-Feng Lu, Nung-Yu Hsu, Tein-Yao Chang, Yuan-Fan Chin, Ping-Cheng Liu, Jir-Mehng Lo, Yeh B. Wu, Jinn-Moon Yang, Cheng Huang
Summary: The study found that the flavonoid ugonin J (UJ) has therapeutic activity against SARS-CoV-2 infection by inhibiting the virus protease and displaying anti-inflammatory properties. UJ shows strong interactions with the active site of the virus protease and has a low cytotoxic effect, making it a promising direct-acting antiviral agent for treating SARS-CoV-2.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shruti Koulgi, Vinod Jani, Mallikarjunachari Uppuladinne, Uddhavesh Sonavane, Asheet Kumar Nath, Hemant Darbari, Rajendra Joshi
Summary: The COVID-19 pandemic caused numerous deaths worldwide, with the causative agent being SARS-CoV-2 and its main protease as a key therapeutic target. Drug repurposing may be effective in targeting this virus, and simulation and structural analysis can help in identifying relevant drug molecules for binding to the main protease of SARS-CoV-2.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Chemistry, Medicinal
Irina Stefanelli, Angela Corona, Carmen Cerchia, Emilia Cassese, Salvatore Improta, Elisa Costanzi, Sveva Pelliccia, Stefano Morasso, Francesca Esposito, Annalaura Paulis, Sante Scognamiglio, Francesco Saverio Di Leva, Paola Storici, Margherita Brindisi, Enzo Tramontano, Rolando Cannalire, Vincenzo Summa
Summary: Despite the availability of vaccines, monoclonal antibodies, and restrictions, there is a strong demand for new antiviral drugs that are both effective and safe against COVID-19. This study describes the design, synthesis, and biological activity of peptidomimetic covalent reversible inhibitors targeting the viral 3-chymotrypsin-like protease (3CLpro) which is essential for viral replication. These inhibitors showed potent activity against multiple coronaviruses, including SARS-CoV-2, MERS-CoV, and HCoV 229. The results suggest that these inhibitors have the potential to be developed as broad-spectrum antiviral drugs against future coronavirus pandemics.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hongbo Liu, Fei Ye, Qi Sun, Hao Liang, Chunmei Li, Siyang Li, Roujian Lu, Baoying Huang, Wenjie Tan, Luhua Lai
Summary: The study showed that Scutellaria baicalensis and its major component baicalein can inhibit the activity of SARS-CoV-2, demonstrating potential as broad-spectrum anti-coronavirus drugs in both in vitro and cell culture settings.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Chun-Chi Chen, Xuejing Yu, Chih-Jung Kuo, Jian Min, Sizhuo Chen, Lixin Ma, Ke Liu, Rey-Ting Guo
Summary: Coronaviruses are RNA viruses that cause severe respiratory syndromes, with SARS-CoV-2 causing the COVID-19 pandemic. The 3CLpro enzyme is a crucial target in the virus life cycle, with inhibitors showing promise in treating CoV diseases.
Article
Chemistry, Multidisciplinary
Xin Ye, Yuhua Li, Lina Guo, Yiling Yao, Rouyu Zhu, Shuang Wei, Hua Diao, Zhiyu Shao
Summary: This study synthesized 19 thiazolidinedione derivatives as potential 3C-like protease inhibitors and screened their activity in vitro using a fluorescence resonance energy transfer enzymatic screening model. Active inhibitors with diverse activity were found in this series of compounds, and two representative inhibitors with potent inhibitory activity were highlighted.
JOURNAL OF CHEMICAL RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Bo Qin, Gregory B. Craven, Pengjiao Hou, Julian Chesti, Xinran Lu, Emma S. Child, Rhodri M. L. Morgan, Wenchao Niu, Lina Zhao, Alan Armstrong, David J. Mann, Sheng Cui
Summary: This study successfully applied quantitative irreversible tethering (qIT) to identify fragments that can inhibit the proteases of Enterovirus 71 and SARS-CoV-2, and found that the inhibition could disrupt the active dimeric unit.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Xin Chen, Ke Chen, Zhaoyong Zhang, Peilan Wei, Lu Zhang, Yunxia Xu, Qili Lun, Yanhong Ma, Fang Wu, Ying Zhang, Yanqun Wang, Jincun Zhao, Yaoqi Zhou, Jian Zhan, Wei Xu
Summary: The PLpro enzyme of SARS-CoV-2 plays a crucial role in virus replication and host immune regulation, making it a potential target for antiviral drugs. In this study, a derivative of chloroxine was found to disrupt the interaction between PLpro and ISG15, showing antiviral effects against SARS-CoV-2 variants.
Article
Chemistry, Medicinal
Arun K. Ghosh, Dana Shahabi, Mackenzie E. C. Imhoff, Satish Kovela, Ashish Sharma, Shin-ichiro Hattori, Nobuyo Higashi-Kuwata, Hiroaki Mitsuya, Andrew D. Mesecar
Summary: This study reports the synthesis and biological evaluation of a series of small molecule SARS-CoV-2 PLpro inhibitors. By comparing the activity of selected compounds in SARS-CoV-1 and SARS-CoV-2, it was found that replacing the carboxamide functionality with sulfonamide derivatives resulted in potent PLpro inhibitors with antiviral activity.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Analytical
Dafu Zhu, Haixia Su, Changqiang Ke, Chunping Tang, Matthias Witt, Ronald J. Quinn, Yechun Xu, Jia Liu, Yang Ye
Summary: Researchers developed a native mass spectrometry-based affinity-selection method for rapid screening of active small molecules from crude herbal extracts used for COVID-19 therapy. Three flavonoids were identified as potential noncovalent inhibitors against 3CLpro, providing a potential strategy for small molecule lead discovery from Traditional Chinese Medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Organic
Korany A. Ali, Naglaa A. Abdel Hafez, Mohamed A. Elsayed, Alhuessin A. Ibrahim
JOURNAL OF HETEROCYCLIC CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Mohamed H. Abo-Ghalia, Gaber O. Moustafa, Abd El-Galil E. Amr, Ahmed M. Naglah, Elsayed A. Elsayed, Ahmed H. Bakheit
Article
Biochemistry & Molecular Biology
Somaia G. Mohammad, Sahar M. Ahmed, Abd El-Galil E. Amr, Ayman H. Kamel
Article
Chemistry, Multidisciplinary
Saad S. M. Hassan, Ayman H. Kamel, Abd El-Galil E. Amr, Heba M. Hashem, E. M. Abdel Bary
Article
Biochemistry & Molecular Biology
Heba E. Hashem, Abd El-Galil E. Amr, Eman S. Nossier, Elsayed A. Elsayed, Eman M. Azmy
Article
Biochemistry & Molecular Biology
Saad S. M. Hassan, Ayman H. Kamel, Abd El-Galil E. Amr, Hisham S. M. Abd-Rabboh, Mohamed A. Al-Omar, Elsayed A. Elsayed
Article
Biochemistry & Molecular Biology
Ayman H. Kamel, Abd El-Galil E. Amr, Hoda R. Galal, Abdulrahman A. Almehizia
Article
Polymer Science
Ayman H. Kamel, Abd El-Galil E. Amr, Nashwa H. Ashmawy, Hoda R. Galal, Abdulrahman A. Almehizia, Teraze A. Youssef, Mohamed A. Al-Omar, Ahmed Y. A. Sayed
Article
Biochemistry & Molecular Biology
Essam Ezzeldin, Nisreen F. Abo-Talib, Marwa H. Tammam, Yousif A. Asiri, Abd El-Galil E. Amr, Abdulrahman A. Almehizia
Article
Chemistry, Analytical
Ayman H. Kamel, Abd El-Galil E. Amr, Hoda R. Galal, Mohamed A. Al-Omar, Abdulrahman A. Almehizia
Article
Chemistry, Analytical
Hisham S. M. Abd-Rabboh, Ayman H. Kamel, Abd El-Galil E. Amr
Article
Biochemistry & Molecular Biology
Ayman H. Kamel, Abd El-Galil E. Amr, Mohamed A. Al-Omar, Abdulrahman A. Almehizia
Article
Biochemistry & Molecular Biology
Abde El-Galil E. Amr, Ayman H. Kamel, Abdulrahman A. Almehizia, Ahmed Y. A. Sayed, Hisham S. M. Abd-Rabboh
Summary: All-solid-state potentiometric sensors, especially those utilizing PEDOT/PSS as the ion-to-electron transducer material, exhibit excellent performance characteristics in the rapid detection of harmine. The sensors show enhanced stability and cost-effectiveness, promising a wide range of potential applications in pharmaceutical and forensic analysis.
Review
Chemistry, Organic
Ashraf M. Mohamed, Wael A. El-Sayed, Alhussein A. Ibrahim, Naglaa A. Abdel-Hafez, Korany A. K. Ali, Salwa F. Mohamed
ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL
(2021)
Article
Polymer Science
Hisham S. M. Abd-Rabboh, Abd El-Galil E. Amr, Abdulrahman A. Almehizia, Ayman H. Kamel
Summary: The application of ion-selective electrodes in pharmaceutical analysis has expanded due to their high selectivity, low cost, and sensitivity. All-solid-state potentiometric selective electrodes designed are cost-effective, reliable, and robust, and have been successfully used for pholcodine determination. The sensors exhibit high sensitivity and can be used for fast and low-cost assessment of pholcodine levels.