Article
Biochemistry & Molecular Biology
Tien-Ching Lee, Hui-Ting Chen, I-Chun Tai, Li-Ting Kao, Ming-Hsin Hung, Chung-Hwan Chen, Yin-Chih Fu, Yan-Hsiung Wang, Chih-Ming Kao, Je-Ken Chang, Mei-Ling Ho
Summary: The simvastatin derivative KMUHC-01 has been found to have a positive effect on promoting bone defect healing, with lower cytotoxicity compared to simvastatin. The new bone microstructure showed improved bone volume and biomechanical tests revealed increased maximal strength in the KMUHC-01 group. Further studies are needed to determine the bioavailability and bone-forming efficacy of KMUHC-01 through systemic administration.
Article
Biochemistry & Molecular Biology
Corina Vater, Elisabeth Mehnert, Henriette Bretschneider, Julia Bolte, Lisa Findeisen, Lucas-Maximilian Matuszewski, Stefan Zwingenberger
Summary: This study investigated the bone regenerative potential of the novel selective EP4 prostaglandin receptor agonist KMN-159, and found that KMN-159 can stimulate bone formation in this model system, showing a dose-dependent effect.
Article
Engineering, Biomedical
M. Bouyer, C. Garot, P. Machillot, J. Vollaire, V Fitzpatrick, S. Morand, J. Boutonnat, V Josserand, G. Bettega, C. Picart
Summary: The study suggests that using 3D-printed PLA scaffolds loaded with reduced doses of BMP-2 may be a safe and simple solution for large bone defects in the clinic. The dose of BMP-2 delivered from the scaffold significantly influences the amount of regenerated bone and the repair kinetics, showing good bone repair outcomes.
MATERIALS TODAY BIO
(2021)
Article
Biochemistry & Molecular Biology
Bruno Zwingenberger, Corina Vater, Roland L. Bell, Julia Bolte, Elisabeth Mehnert, Ronny Bruenler, Dilbar Aibibu, Stefan Zwingenberger
Summary: The study found that CHS scaffolds exhibited higher osteogenic properties in vitro, but resulted in lower bone-healing outcomes compared to CBA and MCM in vivo. Future improvements on CHS scaffolds are needed for better mechanical stability and osteoconductivity.
Article
Biotechnology & Applied Microbiology
Shaowei Zheng, Haobo Zhong, Hao Cheng, Xu Li, Guowei Zeng, Tianyu Chen, Yucong Zou, Weile Liu, Chunhan Sun
Summary: In this study, a TEOS-hydrogel was designed with self-healing property, low swelling rate, degradability, and good biocompatibility, and capable of inducing osteogenic differentiation of bone marrow mesenchymal stem cells. In rabbit models, the TEOS-containing hydrogel accelerated bone regeneration, demonstrating its potential for treating critical-size segmental bone defects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Sanika Suvarnapathaki, Xinchen Wu, Tengfei Zhang, Michelle A. Nguyen, Anastasia A. Goulopoulos, Bin Wu, Gulden Camci-Unal
Summary: Recent innovations in bone tissue engineering have introduced biomaterials that generate oxygen to substitute vasculature. This study demonstrates a novel oxygen-generating tissue scaffold with predictable oxygen release kinetics and modular material properties. The scaffolds showed consistent tissue viability, metabolic activity, and osteogenic differentiation in both in vitro and in vivo experiments.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
S. Kotagudda Ranganath, M. Schlund, Jerome Delattre, J. Ferri, F. Chai
Summary: Most existing preclinical models for evaluating the biosafety and bone-regeneration efficacy of bone substitute materials (BSMs) or tissue engineering (TE) constructs have limitations. This study developed a novel double-site critical-sized defect (CSD) model in one rabbit, which reduces the number of animals needed and avoids the influence of individual differences and evaluation bias.
MATERIALS TODAY BIO
(2022)
Article
Nanoscience & Nanotechnology
Xinchen Wu, Olivia Gauntlett, Tengfei Zhang, Sanika Suvarnapathaki, Colleen McCarthy, Bin Wu, Gulden Camci-Unal
Summary: Scaffold-based bone regeneration using eggshell microparticles in protein-derived hydrogels significantly promotes bone regeneration by inducing osteogenic differentiation and supporting tissue infiltration over a 12-week period.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Nikola Stokovic, Natalia Ivanjko, Igor Erjavec, Anita Breski, Mihaela Peric, Slobodan Vukicevic
Summary: The study found that adding Zoledronate (ZOL) to autologous bone graft substitute (ABGS) enhances the quality and quantity of newly formed bone, and also prolongs its longevity to a certain extent.
Article
Agriculture, Multidisciplinary
Yanjie Wang, Jinghe Sun, Yanqi Zhang, Wenzhuan Liu, Sheng Yang, Jingfeng Yang
Summary: The combined use of SP-2 and BMP-2 enhanced osteoblastic differentiation by increasing ALP expression and mineralization speed, leading to higher levels of BMP-2, Runx-2, COL-I, OCN, and OPN expression. SP-2 acted as a more effective enhancer by increasing the binding efficiency between BMP-2 and its cell surface receptor, proving to be most effective at a dose of 5 μg/mL.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Veterinary Sciences
Ahmed Abdelrahiem Sadek, Mahmoud Abd-Elkareem, Hani Nasser Abdelhamid, Samia Moustafa, Kamal Hussein
Summary: The UiO-66 nanomaterial demonstrates excellent cytocompatibility and stimulates osteoblast functions, indicating its potential as a biomaterial for bone tissue engineering. In vivo experiments show enhanced osteogenesis and healing of bone defects, making UiO-66 a promising candidate for bone repair.
BMC VETERINARY RESEARCH
(2022)
Article
Materials Science, Multidisciplinary
Xinye Chen, Shuang Wang, Xiaohui Zhang, Yuanman Yu, Jing Wang, Changsheng Liu
Summary: Bone fractures in aging patients often have slow healing rates and high complication rates. Injecting a fibrin gel with sulfated chitosan nanoparticles can improve bone regeneration and reduce inflammation at the fracture site. This gel also regulates macrophage function and effectively treats fractures in postmenopausal mice.
APPLIED MATERIALS TODAY
(2022)
Article
Biochemistry & Molecular Biology
Chih-Hsiang Fang, Chung-Kai Sun, Yi-Wen Lin, Min-Chih Hung, Hung-Ying Lin, Ching-Hung Li, I-Ping Lin, Hung-Chen Chang, Jui-Sheng Sun, Jenny Zwei-Chieng Chang
Summary: In this study, a GHMS scaffold was fabricated and evaluated for its effectiveness in bone regeneration in a rat alveolar bone defect model. The GHMS showed good biocompatibility, promoted osteogenic gene and protein expressions, and demonstrated superior bone regeneration compared to other groups in the defect model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Ye Li, Kegong Xie, Chong Wang, Chengliang Yang, Ke Huang, Feng Li, Chuanchuan Zheng, Jian Chen, Shujun Dong, Guangfeng Deng, Gege Huang, Qiaoyan Lu, Jia Liu, Kai Li, Yujin Tang, Liqiang Wang
Summary: The study developed a porous CFZ/β-tricalcium phosphate/poly lactic-co-glycolic acid scaffold for treating critical-sized bone defects, showing promising results in promoting bone and blood vessel growth, and demonstrating for the first time that CFZ-loaded scaffolds could treat nonunion of bone defect by activating the Wnt/β-catenin signaling pathway.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Article
Chemistry, Multidisciplinary
Ruijing Chen, Taojin Feng, Shi Cheng, Ming Chen, Yi Li, Zihui Yu, Ziying Xu, Pengbin Yin, Licheng Zhang, Peifu Tang
Summary: Exosomes derived from human adipose-derived stem cells (hADSCs) can preferentially accumulate at bone, especially at the calvarial defect site, when intravenously injected. Exosomes treatment promotes osteogenesis and bone defect repair. Moreover, exosomes from 3D-cultured hADSCs exhibit superior bone-targeting effects and repair capacity compared to those derived from 2D-cultured hADSCs.
Article
Materials Science, Biomaterials
Alfonso Bayon-Fernandez, Alejandro Mendez-Ardoy, Carmen Alvarez-Lorenzo, Juan R. Granja, Javier Montenegro
Summary: This article introduces the design principles of a new family of self-assembling cyclic octapeptides that can be used as scaffolds for the development of self-healing hydrogels. By inserting different aromatic aldehyde pendants to modulate the hydrophobic/hydrophilic balance and fine-tune the properties of the resulting gel, self-healable hydrogels with viscoelastic properties can be formed.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Instruments & Instrumentation
Ana F. F. Pereira-da-Mota, Maria Vivero-Lopez, Piyush Garg, Chau-Minh Phan, Angel Concheiro, Lyndon Jones, Carmen Alvarez-Lorenzo
Summary: This study aimed to evaluate the release profiles of two drug-loaded contact lenses (CLs) in a 3D in vitro eye blink model and compare them with in vivo profiles. The results showed that the release of both drugs was more sustained and lower in the 3D eye model compared to in vitro release in vials. The findings suggest that the current 3D printed eye blink model could be a useful tool to measure the release of ophthalmic drugs from medicated CLs, but further optimization is needed.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Miriam Gonzalez-Gomez, Ricardo Reyes, Ma del Carmen Damas-Hernandez, Xiomara Plasencia-Cruz, Ibrahim Gonzalez-Marrero, Rafael Alonso, Aixa R. Bello
Summary: The study found that prolonged exposure to the environmental estrogenic substance bisphenol-A (BPA) can negatively affect the reproductive axis, resulting in accelerated sexual maturation and a shorter fertile life in offspring.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Carmen Alvarez-Lorenzo, Maria Vivero-Lopez, Angel Concheiro
Summary: This study demonstrates the spontaneous formation of anisotropic gold nanoparticles (AuNPs) inside preformed contact lenses (CLs) without the need for additional reductant agents. The formation of AuNPs was achieved through a precise tuning of the monomeric composition, saline concentration, and the use of steam heat sterilization. The potential applications of incorporating AuNPs in CLs in the ocular field for prophylaxis, therapy, and diagnosis are highlighted. The developed CLs also exhibit photoresponsiveness and efficient filtering of highly penetrant light.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Carmela Tommasino, Giulia Auriemma, Carla Sardo, Carmen Alvarez-Lorenzo, Emilia Garofalo, Silvana Morello, Giovanni Falcone, Rita P. Aquino
Summary: In this study, a PCL/INU-PLA hybrid biomaterial was prepared, which showed improved surface properties, swelling ability, and in vitro biodegradation rate compared to PCL alone. The 3D printed scaffolds exhibited dimensional and structural parameters close to the digital model and showed favorable results in biocompatibility and osteogenic activity tests.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Polymer Science
Kathleen Abigail Montoya-Villegas, Mirian Angelene Gonzalez-Ayon, Carmen Alvarez-Lorenzo, Angel Licea-Claverie, Emilio Bucio, Alejandro Ramirez-Jimenez
Summary: Thermo-responsive, hydrophilic, hemo- and biocompatible poly(DEGMA-co-OEGMA) was grafted onto silicone rubber using the gamma-radiation pre-irradiation method. The size of the grafted chains was controlled using a RAFT agent. The grafted materials showed enhanced hydrophilicity and excellent hemo- and cytocompatibility, indicating potential applications in the biomedical field.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Pharmacology & Pharmacy
Axel Kattar, Ana Quelle-Regaldie, Laura Sanchez, Angel Concheiro, Carmen Alvarez-Lorenzo
Summary: The aim of this study was to develop cationic niosomes for the ocular delivery of epalrestat, a drug used in the treatment of diabetic eye damage. The niosomes were characterized and found to have a size of 80 nm, a charge ranging from -23 to +40 mV, and a spherical shape. The encapsulation efficiency of the niosomes was 99.76% and they showed a sustained release of the drug over 20 days. The niosomes were non-irritating, non-toxic, and showed higher permeation in the sclera compared to the unencapsulated drug.
Review
Engineering, Biomedical
Mariana Zarur, Alejandro Seijo-Rabina, Alvaro Goyanes, Angel Concheiro, Carmen Alvarez-Lorenzo
Summary: This review focuses on the recent advances in pH-responsive polymer-based scaffolds that have been demonstrated in vivo to be suitable for wound and bone healing. pH plays a critical role in the tissue regeneration process, and small changes can significantly impact the healing process. Various strategies, including changes in ionization of natural polymers, ionizable monomers and clays, reversible cross-linkers, coatings, and production of CO2 gas, have been tested to prepare pH-responsive scaffolds. These techniques have shown promise in achieving biocompatible and environment-friendly scaffolds that can be translated to clinical applications.
ACTA BIOMATERIALIA
(2023)
Review
Biochemistry & Molecular Biology
Federica Ragucci, Francesca Sireci, Francesco Cavallieri, Jessica Rossi, Giuseppe Biagini, Giovanni Tosi, Chiara Lucchi, Rodolfo Molina-Pena, Natalia Helen Ferreira, Mariana Zarur, Alba Ferreiros, William Bourgeois, Francois Berger, Miguel Abal, Audrey Rousseau, Frank Boury, Carmen Alvarez-Lorenzo, Emmanuel Garcion, Anna Pisanello, Giacomo Pavesi, Corrado Iaccarino, Luca Ghirotto, Maria Chiara Bassi, Franco Valzania
Summary: Nanotechnology shows promise in cancer treatment, particularly in the field of neuro-oncology. It is important to explore clinicians' intentions and perspectives on using this new technology in clinical practice.
Meeting Abstract
Cell & Tissue Engineering
Patricia Diaz-Rodriguez, Patricia Garcia-Garcia, Ricardo Reyes, Carmen Evora, Araceli Delgado
TISSUE ENGINEERING PART A
(2023)
Review
Materials Science, Biomaterials
Artur Filipe Rodrigues, Catarina Rebelo, Tiago Reis, Susana Simoes, Liliana Bernardino, Joao Peca, Lino Ferreira
Summary: Neurological disorders pose a major challenge in the medical field due to the inefficacy of current treatments. Despite efforts to regulate neuronal activity and promote tissue regeneration, relapse and progression remain common. Recent advancements in drug delivery systems and neuromodulation strategies show promise in addressing these limitations, but lack precision. External stimuli like electromagnetic fields and ultrasound have been used for non-invasive neuromodulation, but lack the spatial resolution of light stimulation. This review explores the potential of light-responsive nanomaterials for on-demand drug delivery and neuromodulation in brain stimulation and regeneration.
BIOMATERIALS SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Paola Carou-Senra, Lucia Rodriguez-Pombo, Atheer Awad, Abdul W. Basit, Carmen Alvarez-Lorenzo, Alvaro Goyanes
Summary: Inkjet printing (IJP) enables precise material deposition for personalized medicine and drug delivery. This review explores the formulation and components of pharma-inks, various substrate materials, and potential improvements through hardware, software, and artificial intelligence integration. Further exploration and optimization are needed to fully harness the healthcare capabilities of IJP.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Izeia Lukin, Itsasne Erezuma, Patricia Garcia-Garcia, Ricardo Reyes, Carmen Evora, Firoz Babu Kadumundi, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Bone tissue engineering has addressed the clinical need for bone fractures by using synthetic saponite nanoclays to reinforce gelatin-based hydrogel scaffolds, promoting cell proliferation and differentiation, and serving as a drug delivery system.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
