Article
Biotechnology & Applied Microbiology
Keisuke Tsumura, WeiLin Hsu, Masahiro Mimura, Aiko Horiuchi, Kentaro Shiraki
Summary: This study demonstrates a method to decrease the viscosity of antibody solutions by using protein-polyelectrolyte complexes. By adding poly-L-glutamic acid, the viscosity of the antibody solution can be significantly reduced, forming either a crowded solution or a soluble solution. These solutions can be fully redissolved by the addition of phosphate saline buffer.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Medicine, Research & Experimental
Chengnan Huang, Linc Chen, Lutz Franzen, Juliane Anderski, Feng Qian
Summary: This study explored the use of suspension as a formulation form for highly concentrated proteins to reduce viscosity and maintain stability. Spray drying was demonstrated as an effective process to prepare particles for suspension, which showed good physical stability and antibody binding affinity. mAbs recovered from the suspension maintained their conformational structure.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Chuncao Zhao, Zhihan Zhu, Xingchen Cao, Feng Pan, Fang Li, Man Xue, Yilin Guo, Yanhong Zhao, Jia Zeng, Yu Liu, Ziyi Yang, Yan Liu, Fuzheng Ren, Linglin Feng
Summary: This study aimed to determine the important factors affecting the injectability of microparticle delivery systems and establish a suitable injection system with lower injection force and higher discharge rate. The effects of various parameters were evaluated, and the results showed that vehicle viscosity and injection speed have varying effects on discharge rate and injection force, while particle size and needle gauge have substantial influence on injectability. The evaluation method developed in this study can promote the clinical translation of microparticle delivery systems.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Medicine, Research & Experimental
Charles G. Starr, Emily K. Makowski, Lina Wu, Brendan Berg, Jonathan S. Kingsbury, Yatin R. Gokarn, Peter M. Tessier
Summary: CS-SINS is a method that measures colloidal self-interactions at ultradilute antibody concentrations, predicting antibody developability issues at high concentrations, enabling large-scale, high-throughput selection of developable antibodies during early discovery stages.
MOLECULAR PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Xin Feng, Kai-Wei Wu, Vishnu Balajee, Jesse Leissa, Muhammad Ashraf, Xiaoming Xu
Summary: Opioid medications are widely used for treating moderate to severe pain, but they are often misused and abused through various methods such as manipulation and injection. The abuse-deterrent formulations of these drugs can affect their ease of injection, with factors such as needle gauge size, needle length, and syringe barrel size playing important roles.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Ying-Ying Pei, Jin-Tao Wang, Lin Yuan, Yi Luo, Xin-Yue Niu, Xing Rong, Lin Jin, Qing-Feng Li
Summary: This study reports a novel multicolor tunable, injectable, and biodegradable protein-based lanthanide luminescent hydrogel. The hydrogel was formed by denaturing BSA with urea and breaking the disulfide bonds in BSA with TCEP. The resulting crosslinked network was further enhanced by the reaction between lanthanide complexes and the remaining thiols in BSA. The hydrogel showed promising rheological properties, luminescent performances, injectability, and biodegradability. This work provides a feasible strategy for the design and fabrication of multifunctional protein luminescent hydrogels for potential applications in biomedicine, optoelectronics, and information technology.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Physical
Ethan P. Kamphaus, Perla B. Balbuena
Summary: Lithium-sulfur batteries offer significant improvements over traditional lithium-ion batteries but still face challenges such as the polysulfide shuttle effect. Introducing hydrofluorinated ethers into the electrolyte has shown potential in enhancing battery performance and modifying the structure of dissolved polysulfide species.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Pharmacology & Pharmacy
Naoual Dahmana, Pierre-Louis Destruel, Samantha Facchetti, Vanessa Braun, Vanessa Lebouc, Zana Marin, Sulabh Patel, Gregoire Schwach
Summary: This study proposes the use of a reversible protein-polyelectrolyte complex (RPC) approach to develop high concentration formulations of biologics. The RPC concept is versatile and applicable to a wide range of therapeutic protein formats, without affecting their stability. High concentration formulations of up to 200 mg/mL and 250 mg/mL were achieved using RPC suspensions in aqueous buffers and non-aqueous solvents, respectively.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Shreya S. Kulkarni, Sajal M. Patel, Raj Suryanarayanan, Joseph V. Rinella Jr, Robin H. Bogner
Summary: Reconstitution time of lyophilized protein formulations is complex and dependent on various factors. Partially crystalline cakes exhibit better reconstitution characteristics, with the inclusion of crystallizable excipients proving beneficial in speeding up reconstitution.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Md Shahriar, Ankit Rewanwar, Pankaj Rohilla, Jeremy Marston
Summary: The study found that the counterpressure during injection is significantly higher for intradermal injections compared to intramuscular injections, sometimes by an order of magnitude. Additionally, the volumetric flow rate and needle size play important roles in affecting counterpressure during injection. Furthermore, the addition of adjuvants only affects tissue back pressure in the case of subcutaneous injections.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Chemistry, Medicinal
Christoph Marschall, Madlen Witt, Bernhard Hauptmeier, Wolfgang Friess
Summary: High concentration protein formulations for subcutaneous injection are important in the pharmaceutical industry. Protein powder suspensions in non-aqueous vehicles, such as semifluorinated alkanes, enable high concentration formulations with low viscosity and low injection forces. In contrast, suspensions prepared with cryo-milled powder have higher viscosities and cannot be injected at the same concentration.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Polymer Science
Bryce A. Thurston, Gary S. Grest, Mark J. Stevens
Summary: The overlap concentration of sodium polystyrene sulfonate in water is calculated using atomistic and coarse grained simulations. The simulations are in agreement with experimental data, but the chains are not fully stretched even for large chain lengths, indicating that the experimental systems are not in the scaling regime and longer chains are required.
Article
Chemistry, Medicinal
W. Jiskoot, Andrea Hawe, Tim Menzen, David B. Volkin, Daan J. A. Crommelin
Summary: Although many subcutaneously delivered, high-concentration antibody formulations have been approved and widely used, formulation scientists still face challenges in developing new monoclonal antibody-based drugs. These challenges include pharmaceutical attributes, clinical performance, and patient experience. This commentary focuses on the obstacle of maximizing the dose of the drug during HCAF development.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Chemistry, Applied
Yanjun Zhang, Geping Shu, Shansong Gao, Hongxue Wang
Summary: The concentration of coal-oil slurry significantly affects its viscosity and stability, with proper preheating leading to higher concentration. Increasing preheating temperature and time can decrease the viscosity of the slurry, improving its stability.
FUEL PROCESSING TECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Rajat Dandekar, Arezoo M. Ardekani
Summary: Concentrated monoclonal antibody solutions exhibit high viscosity due to fluid occlusion from trapped solvent molecules within the boundaries formed by aggregated antibodies. A theory based on antibody molecule geometry and aggregate morphology can be used to predict antibody viscosity. Controlling drug viscosity can be achieved at the molecular design stage using insights from the viscosity equation.
MOLECULAR PHARMACEUTICS
(2021)
Article
Pharmacology & Pharmacy
Roman Mathaes, Gerhard Winter, Teruna J. Siahaan, Ahmed Besheer, Julia Engert
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2015)
Article
Pharmacology & Pharmacy
Robert Liebner, Sarah Bergmann, Thomas Hey, Gerhard Winter, Ahmed Besheer
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2015)
Article
Chemistry, Medicinal
Robert Liebner, Martin Meyer, Thomas Hey, Gerhard Winter, Ahmed Besheer
JOURNAL OF PHARMACEUTICAL SCIENCES
(2015)
Article
Pharmacology & Pharmacy
Elisabeth Haertl, Nitin Dixit, Ahmed Besheer, Devendra Kalonia, Gerhard Winter
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2013)
Article
Pharmacology & Pharmacy
Robert Liebner, Roman Mathaes, Martin Meyer, Thomas Hey, Gerhard Winter, Ahmed Besheer
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2014)
Review
Pharmacology & Pharmacy
Roman Mathaes, Gerhard Winter, Ahmed Besheer, Julia Engert
EXPERT OPINION ON DRUG DELIVERY
(2015)
Article
Pharmacology & Pharmacy
Roman Mathaes, Gerhard Winter, Julia Engert, Ahmed Besheer
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2013)
Article
Pharmacology & Pharmacy
Roman Mathaes, Gerhard Winter, Ahmed Besheer, Julia Engert
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2014)
Article
Pharmacology & Pharmacy
Matthaeus Noga, Daniel Edinger, Ernst Wagner, Gerhard Winter, Ahmed Besheer
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2014)
Article
Engineering, Biomedical
Matthaeus Noga, Daniel Edinger, Ernst Wagner, Gerhard Winter, Ahmed Besheer
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2014)
Article
Chemistry, Medicinal
Elisabeth Haertl, Gerhard Winter, Ahmed Besheer
JOURNAL OF PHARMACEUTICAL SCIENCES
(2013)
Article
Chemistry, Medicinal
Roman Mathaes, Christian Hildebrandt, Gerhard Winter, Julia Engert, Ahmed Besheer
JOURNAL OF PHARMACEUTICAL SCIENCES
(2013)
Article
Chemistry, Multidisciplinary
Tim Serno, Elisabeth Haertl, Ahmed Besheer, Reinhard Miller, Gerhard Winter
PHARMACEUTICAL RESEARCH
(2013)
Proceedings Paper
Chemistry, Applied
Ahmed Besheer, Robert Liebner, Martin Meyer, Gerhard Winter
TAILORED POLYMER ARCHITECTURES FOR PHARMACEUTICAL AND BIOMEDICAL APPLICATIONS
(2013)
Article
Pharmacology & Pharmacy
Greta Camilla Magnano, Marika Quadri, Elisabetta Palazzo, Roberta Lotti, Francesca Loschi, Stefano Dall'Acqua, Michela Abrami, Francesca Larese Filon, Alessandra Marconi, Dritan Hasa
Summary: This study aimed to investigate the loading of sildenafil citrate in three commercial transdermal vehicles using 3D full-thickness skin equivalent and compare the results with permeability experiments using porcine skin. The results showed that the results obtained using the 3D skin equivalent were comparable to those obtained using porcine skin, suggesting that the 3D skin model can be a valid alternative for ex-vivo skin absorption experiments.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)
Article
Pharmacology & Pharmacy
James W. Mckeage, Andrew Z. H. Tan, Andrew J. Taberner
Summary: Needle-free jet injection is a promising alternative drug delivery technique that offers rapid, non-invasive, and large-volume injections. The study presents a prototype multi-orifice nozzle and a computational fluid dynamic model to demonstrate the feasibility and effectiveness of this technology.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2024)