Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Chemistry, Applied
Na Ren, Xin Yu, Aizhu Wang, Na Liang, Zhichao Feng, Chunhui Sun
Summary: The study demonstrates that ScCl3 can enhance proliferation and osteogenic differentiation of rat bone MSCs while reducing adipogenic differentiation to a certain extent through the Wnt/beta-catenin signaling pathway. Appropriate concentrations of ScCl3 show potential in improving bone remodeling and may lead to new strategies for designing composites for biomedical applications in the future.
JOURNAL OF RARE EARTHS
(2022)
Article
Multidisciplinary Sciences
Wei Cao, Xiaohong Yang, Xiao Hua Hu, Jun Li, Jia Tian, RenJun OuYang, Xue Lin
Summary: This study analyzed the biological characteristics of the mandible and mandibular bone marrow mesenchymal stem cells in the bone microenvironment of postmenopausal osteoporosis. It identified miR-344d-3p as a promoter of osteogenic differentiation and inhibitor of adipogenic differentiation, with Dnmt3a potentially being its target gene. These findings are of significant importance in the field of bone reconstruction.
Article
Biochemistry & Molecular Biology
Thorben Sauer, Giulia Facchinetti, Michael Kohl, Justyna M. Kowal, Svitlana Rozanova, Julia Horn, Hagen Schmal, Ivo Kwee, Arndt-Peter Schulz, Sonja Hartwig, Moustapha Kassem, Jens K. Habermann, Timo Gemoll
Summary: This study characterized the differences between MSCs and cells after adipogenic or osteoblastic differentiation at the proteome level. Specific proteins related to bone formation were identified, which could be used for regenerative medicine in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yaqiong Yu, Yoko Uchida-Fukuhara, Yao Weng, Yuhan He, Mika Ikegame, Ziyi Wang, Kaya Yoshida, Hirohiko Okamura, Lihong Qiu
Summary: In this study, the roles of NRP1 and GAG-modifiable NRP1 in adipogenesis were explored in C3H10T1/2 cells. The expression of full-length NRP1 and GAG-modifiable NRP1 increased during adipogenic differentiation, and NRP1 knockdown repressed adipogenesis. JIP4 was found to be involved in adipogenesis by interacting with NRP1. Moreover, the non-GAG-modifiable NRP1 mutant greatly promoted adipogenic differentiation by activating the Akt and ERK1/2 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Oncology
Till Jasper Meyer, Stephan Hackenberg, Marietta Herrmann, Thomas Gehrke, Magdalena Steber, Rudolf Hagen, Norbert Kleinsasser, Agmal Scherzad
Summary: This study investigated the effects of head and neck squamous cell carcinoma (HNSCC) cell lines Cal27 and HLaC78 on the induction of osteogenic and adipogenic differentiation in human mesenchymal stem cells (hMSCs). The results showed that co-culturing hMSCs with Cal27 and HLaC78 cells resulted in both adipogenic and osteogenic differentiation. In addition, adipogenic hMSCs promoted the viability of Cal27 and HLaC78 cells with STAT3 activation as a possible mechanism.
Article
Cell Biology
Konstantin Kulebyakin, Pyotr Tyurin-Kuzmin, Anastasia Efimenko, Nikita Voloshin, Anton Kartoshkin, Maxim Karagyaur, Olga Grigorieva, Ekaterina Novoseletskaya, Veronika Sysoeva, Pavel Makarevich, Vsevolod Tkachuk
Summary: Modern biomedical science requires easy and reliable sources of human cells, with adult mesenchymal stem/stromal cells (MSCs) being a promising option, but immortalized MSCs may have drawbacks that affect their efficacy in studying hormonal sensitivity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Shin Ishikane, Eigo Ikushima, Kazunobu Igawa, Katsuhiko Tomooka, Fumi Takahashi-Yanaga
Summary: The study showed that DIF-1 significantly enhanced adipogenic differentiation but attenuated osteogenic differentiation of BM-MSCs. Results suggest that DIF-1 supports MSC differentiation towards the desired cell fate by enhancing differentiation efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Cell Biology
Chenglong Wang, Sabine Stoeckl, Shushan Li, Marietta Herrmann, Christoph Lukas, Yvonne Reinders, Albert Sickmann, Susanne Graessel
Summary: This study investigated the effects of extracellular vesicles (EVs) isolated from different stages of human bone marrow mesenchymal stem cells (hBMSCs) on the osteogenic and adipogenic differentiation capacity of naive hBMSCs. The results showed that EVs from late-stage osteogenic differentiated hBMSCs promoted osteogenic potential in naive hBMSCs and inhibited their adipogenic differentiation. Proteomic analysis revealed distinct protein profiles in EVs from osteogenic and naive hBMSCs.
Article
Chemistry, Analytical
Yadi Wang, Wentao Dai, Zhixiao Liu, Jixiang Liu, Jie Cheng, Yuanyuan Li, Xueling Li, Jun Hu, Junhong Lu
Summary: A method based on single-cell FTIR microspectroscopy was developed to quantitatively evaluate cellular heterogeneity by calculating the cell-to-cell similarity distance, revealing that infrared phenotypes may reflect dynamic heterogeneity changes in cell populations. This provides an alternative label-free optical approach for quantifying cellular heterogeneity and can be combined with other single-cell analysis tools to understand the genotype-to-phenotype relationship in cellular populations.
ANALYTICAL CHEMISTRY
(2021)
Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Cell Biology
Qingyu Zhang, Jun Dong, Peng Zhang, Dongsheng Zhou, Fanxiao Liu
Summary: Key gene regulatory events were identified in early stages of lineage commitment in BMSCs, with two transcription factors (Tsc22d3 and Epas1) showing elevated expression throughout different stages of three-directional differentiation in rats.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Yuxuan Zhong, Xiang Li, Fanglin Wang, Shoushuai Wang, Xiaohong Wang, Xiaohong Tian, Shuling Bai, Di Miao, Jun Fan
Summary: Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. Exosomes play an important role in promoting tissue regeneration and provide new ideas for adipose tissue engineering research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Xujie Liu, Yakun Wang, Yan He, Xiaofeng Wang, Ranran Zhang, Akash Bachhuka, Rahul Madathiparambil Visalakshan, Qingling Feng, Krasimir Vasilev
Summary: The study reveals that surface chemistry and topography can synergistically influence the morphology, focal adhesion formation, and osteogenic/adipogenic differentiation of human mesenchymal stem cells. This suggests that different material properties can have a significant impact on cell response and highlights the importance of designing functional biomaterials.
ACS APPLIED MATERIALS & INTERFACES
(2021)
