Article
Biology
Ruoxu Chen, Steven M. Sanders, Zhiwei Ma, Justin Paschall, E. Sally Chang, Brooke M. Riscoe, Christine E. Schnitzler, Andreas D. Baxevanis, Matthew L. Nicotra
Summary: This study establishes Hydractinia as a reliable non-bilaterian model system for the study of sex determination and the evolution of sex chromosomes. By generating a linkage map and utilizing deep sequencing, the researchers found pseudoautosomal and non-recombining regions on the X and Y chromosomes of Hydractinia. They also identified genes in the non-recombining region that are specifically expressed in the male gonad. Additionally, the study observed enhanced recombination rates in the female genome and determined that Hydractinia has a haploid chromosome number of n = 15.
Article
Agronomy
Sundas Shahzad, Muhammad Saeed, Shagufta Perveen, Muhammad Azeem, Iqra Sajjad, Shazia Saeed, Muhammad Naeem, Alia Javed, Iqra Sultan, Ukasha Faria, Sana Maryam, Muhammad Zaid Yousaf
Summary: In this study, genetic diversity and population structure of sixty Saccharum spp. hybrids were assessed using SSR markers. The results showed that SSR markers were effective tools for evaluating genetic diversity and varietal fingerprinting in sugarcane hybrids, which could aid in selecting the best parents for planned crosses.
GENETIC RESOURCES AND CROP EVOLUTION
(2022)
Article
Plant Sciences
Huahao Jiang, Muhammad Waseem, Yong Wang, Sana Basharat, Xia Zhang, Yun Li, Pingwu Liu
Summary: Sugarcane is an important agricultural crop used for sugar production and biofuel. The genetic complexity of sugarcane makes it a challenging crop to improve. This study developed SSR markers from sugarcane ESTs, which can be used for genetic characterization and breeding efforts. Functional annotation analysis showed that the markers cover molecular, cellular, and biological pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yiwei Zhou, Xue Wei, Farhat Abbas, Yunyi Yu, Rangcai Yu, Yanping Fan
Summary: In this study, a large number of SSR markers were identified in the genome of Hedychium coronarium 'ZS', and genetic diversity analysis and genotype identification were conducted among different species, showing clear regional distribution characteristics. These new SSR markers will provide important references for genetic diversity analysis and breeding of Hedychium.
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS
(2021)
Article
Microbiology
Shuhe Wang, Chaofan Gao, Qiuyu Sun, Qi Liu, Cuicui Wang, Fangfang Guo, Zhanhong Ma
Summary: This study analyzed the genetic diversity and population structure of Puccinia striiformis f. sp. tritici (Pst) isolates from different wheat cultivars in China. The findings suggest that geographic and environmental differences play a significant role in the genetic differentiation of Pst, rather than wheat genotype.
Article
Microbiology
Shouji Yamamoto, Sunao Iyoda, Makoto Ohnishi
Summary: Hypermutable simple sequence repeats (SSRs) play a key role in phase variation of Campylobacter jejuni, leading to extreme cell surface diversity within bacterial populations. Natural cotransformation is an effective method for genetically manipulating C. jejuni genomes, allowing for the introduction of multiple mutations and stable phase-locked strains. This approach provides a platform for elucidating the mechanisms underlying phase-variable expression in C. jejuni.
Article
Agriculture, Multidisciplinary
Hakan Guven, Sydney E. Everhart, Rita Milvia De Miccolis Angelini, Hilal Ozkilinc
Summary: The study found significant differentiation among samples from Turkey, the USA, and Italy based on microsatellite analysis. Samples from Turkey showed low diversity, while samples from the USA had the highest genetic diversity values. Samples from Italy were all found to be a single haplotype, distinct from samples from other locations.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
Chao Shen, Nian Wang, De Zhu, Pengcheng Wang, Maojun Wang, Tianwang Wen, Yu Le, Mi Wu, Tian Yao, Xianlong Zhang, Zhongxu Lin
Summary: The study presents a high-quality genome assembly for Gossypium tomentosum, revealing extensive genetic variations and constructing dense genetic maps. Candidate genes associated with recombination and floral transition were identified, providing valuable genomic resources for genetic improvement and breeding in cotton.
Article
Biotechnology & Applied Microbiology
Rezwanuzzaman Laskar, Md Gulam Jilani, Safdar Ali
Summary: The analysis of SSRs in 98 genomes from four genera of the Polyomaviridae family revealed a high prevalence of mono-nucleotide repeats, with T and A being the most common motifs. The distribution of SSRs was mainly in the coding region, especially in the Large T Antigen gene. Viruses with humans and related species as hosts showed a distinct SSR pattern in the AT region, suggesting a predictive marker for host determination during viral evolution.
Article
Oceanography
Nan Ze, Xu Ran, Hou Chunqiang, Zheng Xiaodong
Summary: This study discovered microsatellite loci in O. minor, providing genetic markers for population assessment, management, and conservation of this species.
JOURNAL OF OCEAN UNIVERSITY OF CHINA
(2022)
Article
Optics
Noura Khalil, Amany Sarhan, Mahmoud A. M. Alshewimy
Summary: The paper proposes an effective chaotic color/grayscale image encryption algorithm that combines 2D and 1D chaotic maps, with simple structure, easy implementation, and excellent chaotic features for higher security. Simulation and analysis results show promising security performance and high resistance to statistical and differential attacks.
OPTICS AND LASER TECHNOLOGY
(2021)
Article
Engineering, Electrical & Electronic
Yan Zhao, Jiafeng Ding, Shaobo He, Huihai Wang, Kehui Sun
Summary: In this paper, fully fixed-point digital integrated circuits for discrete memristive systems are designed to have very low circuit area cost, using a two-stage pipeline and multi-cycle architecture. Mathematical models for the discrete memristor and a memristor chaotic map are presented and their dynamics are analyzed. Data flow design is carried out and the feasibility of the fixed-point model is discussed, providing a foundation for further circuit design. Different architectures are designed to obtain optimal results. Gate area and gate counts are provided for the discrete memristor and the discrete memristor map. Additionally, a chaotic pseudo random sequence generator is designed and passes the NIST test, laying the foundation for further applications of the discrete memristor and discrete memristor chaotic systems.
AEU-INTERNATIONAL JOURNAL OF ELECTRONICS AND COMMUNICATIONS
(2023)
Article
Agronomy
Annu Lata, Hemant Kumar Yadav, Narayanan K. Nair
Summary: This study estimated the genetic diversity and population structure of wood-apple samples from 16 states in India using 24 polymorphic SSRs. The results showed moderate to high genetic diversity among wood-apple samples and identified 14 diverse samples and 9 highly informative SSRs that are useful for breeding and conservation of this species.
GENETIC RESOURCES AND CROP EVOLUTION
(2023)
Article
Agriculture, Multidisciplinary
Selda Daler, Rustem Cangi
Summary: The study characterized 50 grape varieties grown in Yozgat using molecular methods and SSR analysis, revealing a high level of diversity in alleles and genetic characteristics. Cluster analysis showed that these grape varieties can be grouped into 2 main clusters, with varying genetic similarity rates among them.
TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
(2022)
Article
Biotechnology & Applied Microbiology
Qingwen Yang, Yujie Jiang, Yuping Wang, Ruilian Han, Zongsuo Liang, Qiuling He, Qiaojun Jia
Summary: Through transcriptome analysis, a large number of SSR loci were discovered in Polygonatum sibiricum, with rich types and high potential for polymorphism. These SSR loci can be used for candidate gene mining and marker-assisted breeding, as well as providing technical methods for molecular identification and marker-assisted breeding of superior varieties in Polygonatum genus.
BIOMED RESEARCH INTERNATIONAL
(2022)
Article
Endocrinology & Metabolism
Qifeng Zhang, Ding Ye, Houpeng Wang, Yaqing Wang, Wei Hu, Yonghua Sun
Article
Biochemistry & Molecular Biology
Mudan He, Ru Zhang, Shengbo Jiao, Fenghua Zhang, Ding Ye, Houpeng Wang, Yonghua Sun
Article
Biochemistry & Molecular Biology
Xianmei Li, Fenghua Zhang, Nan Wu, Ding Ye, Yaqing Wang, Xiaofan Zhang, Yonghua Sun, Yong-An Zhang
JOURNAL OF GENETICS AND GENOMICS
(2020)
Article
Fisheries
Yousef Mohammed, Ding Ye, Mudan He, Houpeng Wang, Yanhua Li, Danxiang Han, Zuoyan Zhu, Yonghua Sun
Summary: The study successfully engineered fish cells and zebrafish embryos to synthesize ketocarotenoids, with significantly increased content in fish models both in vitro and in vivo, demonstrating the potential for animals to manufacture these valuable molecules.
Article
Endocrinology & Metabolism
Yaqing Wang, Ding Ye, Fenghua Zhang, Ru Zhang, Junwen Zhu, Houpeng Wang, Mudan He, Yonghua Sun
Summary: This study demonstrates the functional importance of cyp11a2 in early oogenesis and differentiation of SSCs in zebrafish. Depletion of cyp11a2 results in steroidogenic deficiencies, sex reversal, and infertility in males. The mutation leads to apoptosis of oocytes in developing ovaries and dysfunction of Sertoli cells and Leydig cells in developing testes.
Article
Biology
Fenghua Zhang, Yongkang Hao, Xianmei Li, Yi Li, Ding Ye, Ru Zhang, Xiaosi Wang, Mudan He, Houpeng Wang, Zuoyan Zhu, Yonghua Sun
Summary: With an optimized SSCT procedure, researchers successfully obtained genome-edited sperm derived from Chinese rare minnow in zebrafish, demonstrating the feasibility of surrogate production of gametes from a different subfamily using surrogate reproduction techniques. Knockout of pou5f3 and chd genes in Gr SSCs also led to the successful production of genome-edited sperm. This study opens up new possibilities for the future breeding of commercial fish and livestock.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Binbin Tao, Hongling Hu, Ji Chen, Lu Chen, Daji Luo, Yonghua Sun, Feng Ge, Zuoyan Zhu, Vance L. Trudeau, Wei Hu
Summary: Recent research has identified a new subunit of the SIN3A-HDAC complex, called Sinhcaf, that plays a crucial role in germ plasm aggregation and primordial germ cell (PGC) specification. Sinhcaf-dependent histone deacetylation is essential for proper PGC development, as mutations in sinhcaf result in defects in germ plasm aggregation and decreased PGC abundance. Sinhcaf achieves this by regulating the acetylation status of the kif26ab promoter, which affects its transcription and ultimately PGC specification.
Article
Genetics & Heredity
Yan Zhao, L. Filipe C. Castro, Oscar Monroig, Xiaojuan Cao, Yonghua Sun, Jian Gao
Summary: This study generated a zebrafish mutant lacking ppar γ with CRISPR/Cas9 technology and investigated its regulatory network. The deletion of ppar γ protected against high-fat diet-induced lipid deposition and involved protein kinases related to lipid metabolism.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2022)
Article
Cell Biology
Haibo Xie, Xiaosi Wang, Minjun Jin, Lanqin Li, Junwen Zhu, Yunsi Kang, Zhe Chen, Yonghua Sun, Chengtian Zhao
Summary: Meiosis is crucial for evolution and genetic diversity, and cilia play a critical role in the meiotic recombination process. Our study reveals the involvement of cilia in meiosis and suggests that extracellular signals may regulate meiotic recombination through this organelle.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yao Xiao, Shengbo Jiao, Miao He, Da Lin, Huanyan Zuo, Jiahao Han, Yonghua Sun, Gang Cao, Zhi Chen, Huan Liu
Summary: This study utilizes an integrated epigenomic dataset to identify functional variants associated with orofacial clefts, providing a framework for risk assessment and understanding of clefts.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ru Zhang, Yi-Xuan Tu, Ding Ye, Zhenglong Gu, Zhen-Xia Chen, Yonghua Sun
Summary: This study identifies zebrafish pld6 as a novel germline-specific gene that plays a crucial role in the maintenance and differentiation of germline stem and progenitor cells (GSPCs). Pld6 knockout mutants develop exclusively into infertile males, as the GSPCs fail to differentiate and undergo apoptosis. Furthermore, pld6-depleted GSPCs display impaired mitochondrial fusion, defects in piRNA biogenesis, and transposon suppression.
Article
Developmental Biology
Mudan He, Shengbo Jiao, Ru Zhang, Ding Ye, Houpeng Wang, Yonghua Sun
Summary: This study demonstrates that maternal Nanog regulates oogenesis and early embryogenesis through translational control of maternal mRNA via a mechanism whereby Nanog acts as a transcriptional repressor to suppress transcription of eef1a1l2. The loss of Nanog leads to defects in egg maturation and increased translational activity, which can be rescued by depleting eef1a1l2.
Article
Zoology
Shan-Shan Xu, Yi Li, Hou-Peng Wang, Wen-Bo Chen, Ya-Qing Wang, Zi-Wei Song, Hui Liu, Shan Zhong, Yong-Hua Sun
Summary: This study investigates the role of Stearyl coenzyme A desaturase (SCD) in lipid metabolism and sexual development using a zebrafish scd mutant. The mutant fish displayed increased levels of saturated fatty acids and decreased levels of monounsaturated fatty acids. They also exhibited abnormalities in body size, fat accumulation in the liver, hepatic mitochondrial dysfunction, and cell apoptosis. The mutant males showed defective mating behavior due to genital papillae defects but had functional mature sperm. Overexpression of scd rescued all the observed defects in the mutant fish.
ZOOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Zhe Zhang, Chun Yang, Zixu Wang, Liwei Guo, Yongpan Xu, Ce Gao, Yonghua Sun, Zhenhai Zhang, Jinrong Peng, Minjie Hu, Li Jan Lo, Zhipeng Ma, Jun Chen
Summary: Food digestion requires coordinated cooperation of different digestive organs. In this study, we demonstrated that Wdr5-mediated H3K4 tri-methylation plays a critical role in regulating cell differentiation, proliferation, and apoptosis during zebrafish organogenesis of three major digestive organs. Our findings provide insights into the common molecular mechanisms of cell fate determination in different digestive organs and shed light on the mechanistic basis of human diseases in these organs.
CELL DEATH DISCOVERY
(2023)
Article
Biotechnology & Applied Microbiology
Yousef Mohammed, Ding Ye, Mudan He, Houpeng Wang, Zuoyan Zhu, Yonghua Sun
Summary: This study successfully engineered animal cells to biosynthesize astaxanthin, with significant implications for transgenic animals and the aquaculture sector. After optimization, the concentration of astaxanthin increased significantly, making it a promising application.
BIOENGINEERING-BASEL
(2023)