Article
Multidisciplinary Sciences
Huili Xue, Aili Yu, Na Lin, Xuemei Chen, Min Lin, Yan Wang, Hailong Huang, Liangpu Xu
Summary: The etiopathogenesis of fetal ventriculomegaly is not well understood. This study investigated the correlations between fetal ventriculomegaly and chromosome abnormalities, finding higher detection rates of clinically significant CNVs in non-isolated ventriculomegaly compared to isolated cases. Additionally, SNP arrays were found to effectively identify CNVs and increase the abnormal chromosomal detection rate, particularly in cases of ventriculomegaly accompanied by CNS anomalies.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Ling Zhi Wu, Yuan Ye, Zhi Xuan Wang, Die Ma, Li Li, Guo Hao Xi, Bi Qing Bao, Li Xing Weng
Summary: A novel SNP detection assay has been developed for single-nucleotide discrimination using a nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The study successfully detected the SNP of p53 gene mutation in gastric cancer at femtomolar concentration, offering high sensitivity and spatial resolution for potential applications in early diagnosis and disease prevention.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Zhen Song, Wei Li Ang, Jiri Sturala, Vlastimil Mazanek, Petr Marvan, Zdenek Sofer, Adriano Ambrosi, Caifeng Ding, Xiliang Luo, Alessandra Bonanni
Summary: This study introduces a disposable genosensor utilizing nanosized germananes for sensitive and selective detection of Alzheimer-related SNP, with germanane showing excellent bioanalytical performance. The potential for miniaturization and decentralized analysis is demonstrated, highlighting the versatility and promise of germanene-based nanomaterials in biosensing applications.
ACS APPLIED NANO MATERIALS
(2021)
Article
Microbiology
Malkhan Singh Gurjar, Rashmi Aggarwal, Shekhar Jain, Sapna Sharma, Jagmohan Singh, Sangeeta Gupta, Shweta Agarwal, Mahender Singh Saharan
Summary: The study focused on genetic variations in Indian isolates of Tilletia indica, revealing that KB-11 isolate was highly conserved while KB-18 isolate was the most diverse, indicating a high diversity within the T. indica population.
Article
Chemistry, Multidisciplinary
Yun Zhang, Bo Chen, Duo Chen, Yiming Wang, Qingqing Lu, Jie Tan, Long Chen, Liping Zhou, Weihong Tan, Yanbing Yang, Quan Yuan
Summary: The development of an efficient and sensitive on-site nucleic acid testing method with SNP identification capability is urgently needed due to the large-scale pandemic and fast evolution of SARS-CoV-2 variants. In this study, a multiplexed electrical detection assay based on a PNprobe functionalized FET biosensor was developed for highly sensitive and specific detection and discrimination of SARS-CoV-2 variants. The assay achieved simultaneous detection and identification of key mutations of seven SARS-CoV-2 variants within 15 minutes, with an identification accuracy of 97.1% for 70 simulated throat swab samples.
Article
Chemistry, Multidisciplinary
Jiaoyun Xia, Tong Xu, Jing Qing, Lihua Wang, Junlong Tang
Summary: The study introduced a novel biosensor based on the quenching effect of fluorescence-embedded SYBR Green I (SG) dye and graphene oxide (GO) for detecting single nucleotide polymorphisms (SNPs), which is crucial for early disease diagnosis and drug rational use. By optimizing experimental parameters, the sensor achieved high SNP discrimination and differentiation under optimal conditions, making it a low-cost, label-free, and simple operation method with high SNP resolution at room temperature.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Juneseok You, Kuewhan Jang, Hyunjun Park, Seonwoo Lee, Ahreum Lim, Chanho Park, Kyonghwa Park, Sungsoo Na
Summary: This study developed a method using PNA and RNase H II for real-time PCR to detect KRAS single nucleotide polymorphism. The method exhibited high sensitivity and selectivity, and achieved a synergistic effect by combining the advantages of both techniques. Validation tests with different samples showed the potential of this method in detecting cancer mutations and its applicability in breath biopsy.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Wenjun Jiang, Rashid Aman, Zahir Ali, Gundra S. Rao, Magdy Mahfouz
Summary: This study presents a new peptide nucleic acid (PNA)-based nucleic acid detection toolkit, PNA-Pdx, which provides accurate and rapid detection of specific nucleic acid sequences, including pathogens and genomic sequences, such as SARS-CoV-2, in clinical samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wenjun Jiang, Rashid Aman, Zahir Ali, Gundra S. Rao, Magdy Mahfouz
Summary: PNA-Pdx is a novel peptide nucleic acid (PNA)-based nucleic acid detection toolkit that can accurately and rapidly detect specific nucleic acid sequences. It can also invade double-stranded DNA to produce single-stranded DNA for precise detection of genomic SNPs.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Marcelino Varona, Jared L. Anderson
Summary: Detection of mutations and single-nucleotide polymorphisms is crucial for diagnostic applications, and Loop-mediated isothermal amplification (LAMP) is a powerful tool for this purpose. However, traditional LAMP lacks the ability to distinguish single-nucleotide differences within target sequences. Various approaches have been developed to address this limitation, including primer design modifications and probe-based detection methods.
Article
Biochemistry & Molecular Biology
Maria Radanova, Mariya Levkova, Galya Mihaylova, Rostislav Manev, Margarita Maneva, Rossen Hadgiev, Nikolay Conev, Ivan Donev
Summary: There is a growing interest in studying single nucleotide polymorphisms (SNPs) in microRNA (miRNA) genes, as they may be associated with susceptibility, prognosis, and treatment response in colorectal cancer (CRC). These miRNA-SNPs could serve as non-invasive biomarkers for early detection of CRC. However, contradictory findings have been reported when different research groups investigated the same SNP in a gene for a specific miRNA, highlighting the need for more case-control studies involving participants from different ethnic backgrounds. According to our review, three miRNA-SNPs - miR-146a rs2910164, miR-27a rs895819, and miR-608 rs4919510 - appear to be promising prognostic, diagnostic, and predictive biomarkers for CRC.
Review
Plant Sciences
Zheng Ying, Muhammad Awais, Reshmi Akter, Fengjiao Xu, Sul Baik, Daehyo Jung, Deok Chun Yang, Gi-Young Kwak, Wenying You
Summary: Discriminating plant species, cultivars, and landraces is challenging, but SNP markers have proven to be a reliable tool for differentiating Panax ginseng and similar species. This article provides a cost-effective guide for SNP marker analysis in a traditional laboratory setting.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Cardiac & Cardiovascular Systems
Matthew R. Alexander, Samuel Hank, Bethany L. Dale, Lauren Himmel, Xue Zhong, Charles D. Smart, Daniel J. Fehrenbach, Yuhan Chen, Nitin Prabakaran, Brian Tirado, Megan Centrella, Mingfang Ao, Liping Du, Yu Shyr, Daniel Levy, Meena S. Madhur
Summary: Research suggests that the Trp encoding allele of rs3184504 may be causal for hypertension and renal dysfunction, in part due to the loss of SH2B3-mediated repression of T cell IL-12 signaling leading to enhanced IFNg production.
CIRCULATION RESEARCH
(2022)
Article
Fisheries
Channapha Sakseepipad, Reiko Nozaki, Keisuke Yoshii, Yutaka Fukuda, Kaori Mizuno, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study developed two primer sets to amplify SNP regions of RSIV-type megalocytiviruses, identifying 53 polymorphic sites and clustering 25 isolates into eight haplotypes through phylogenetic analysis. These findings suggest that SNPs in the RSIV genome are a powerful tool for detection and identification of RSIV-type megalocytiviruses.
JOURNAL OF FISH DISEASES
(2021)
Article
Chemistry, Analytical
Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Yunjing Li, Gang Wu, Jingang Liang, Hongfei Gao
Summary: This study developed a specific and sensitive detection method for the cauliflower mosaic virus 35s (CaMV35S) promoter in genetically modified (GM) crops using the nuclease-dead Cas9 (dCas9)/sgRNA system and a clustered regularly interspaced short palindromic repeats (CRISPR)/dCas9-mediated enzyme-linked immunosorbent assay (ELISA). The proposed method offers a specific, sensitive, rapid, and cost-effective detection platform for molecular diagnoses.