Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Multidisciplinary Sciences
Ashley E. Tessnow, Todd M. Gilligan, Eric Burkness, Caroline Placidi De Bortoli, Juan Luis Jurat-Fuentes, Patrick Porter, Danielle Sekula, Gregory A. Sword
Summary: The fall armyworm, Spodoptera frugiperda, is a polyphagous global pest that preferentially feeds on gramineous crops such as corn, sorghum, and pasture grasses. It consists of two genetically distinct host strains known as the corn and rice strains, complicating pest management approaches. Through molecular markers and TaqMan assays, researchers are able to differentiate between strains and interstrain hybrids, allowing for future studies on strain population dynamics and hybrids. Understanding the ecology of S. frugiperda strains is crucial for informing effective management strategies.
Article
Biochemistry & Molecular Biology
Zaidong Hua, Kang Xu, Wei Xiao, Chang Shu, Nana Li, Kaiqiang Li, Hao Gu, Zhe Zhu, Liping Zhang, Hongyan Ren, Qinghua Zeng, Yulong Yin, Yanzhen Bi
Summary: The mature peptide encoded by the Myostatin (MSTN) gene was successfully knocked out in pigs using dual sgRNA-mediated deletion, resulting in skeletal muscle overgrowth and significant alteration of meat production traits. This study is expected to have a wide impact on genetic improvements in food animals.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Veterinary Sciences
Suresh V. Kuchipudi, Meera Surendran Nair, Michele Yon, Abhinay Gontu, Ruth H. Nissly, Rhiannon Barry, Denver Greenawalt, Traci Pierre, Lingling Li, Nagaraja Thirumalapura, Deepanker Tewari, Bhushan Jayarao
Summary: A series of high mortality outbreaks caused by virulent strains of Streptococcus equi subspecies zooepidemicus affected swine herds in the USA and Canada in 2019, requiring rapid and accurate diagnosis. A novel probe-based real-time PCR targeting a conserved region of the SzM gene was developed, showing high sensitivity and specificity for virulent swine isolates without cross reactivity with avirulent strains or other pathogens. The PCR assay demonstrated 100% sensitivity and specificity compared to traditional bacteriological culture methods when tested with clinical samples.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Si-Tong Lv, Kai Gao, Hak Myong Choe, Zheng-Yun Jin, Shuang-Yan Chang, Biao-Hu Quan, Xi-Jun Yin
Summary: This study investigated the effect of Myostatin (MSTN) on the superior rectus (SR) and superior oblique (SO) muscles in pigs. The results showed that MSTN-/- pigs had significantly larger muscle fiber cross-sectional areas compared to wild-type (WT) pigs, and the fiber type in MSTN-/- SO exhibited greater changes compared to SR. Furthermore, the expression of MyoD and MyoG genes was significantly increased in MSTN-/- SO.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Walid Hossain, Mohabbat Hossain, Khalid Arafath, Subarna Sayed Ety, Mahade Hasan Shetu, Mazbahul Kabir, Farjana Akther Noor, Kalissar Mannoor
Summary: The study aimed to reduce the run time of multiplex RT-PCR for detecting SARS CoV-2 N1 and N2 sequences and human RNase P (RP) sequence. By optimizing the Fast PCR protocol and comparing it with conventional PCR, the researchers found that Fast PCR significantly shortened the run time while yielding comparable results to conventional PCR.
Article
Chemistry, Analytical
Yuwei Qiu, Kunlun Jiang, Jingrui Wu, Yung-Kang Peng, Jeong-Wook Oh, Jung- Hoon Lee
Summary: A plasmonic photothermal quantitative immuno-PCR (PPT-qiPCR) assay is developed for biomarker detection using multifunctional plasmonic immunomagnetic nanoparticles (PIMNs). This assay has real-time and end-point dual readout and is simple, time-saving, and inexpensive compared to conventional iPCR platforms. It can quickly discriminate target proteins and improve assay sensitivity with in-situ end-point fluorescence or colorimetric detection using only the human eye.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Jing Tu, Yi Qiao, Yuhan Luo, Naiyun Long, Zuhong Lu
Summary: DNA quantification is crucial in life sciences research, and real-time MDA technology can provide template amount information during whole-genome amplification with a very low detection limit, offering a promising tool to quantify entire DNA without sacrificing sample completeness or introducing redundant steps.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Youngseop Lee, Eun-Sil Yu, Hamin Na, Minhee Kang, Hee Jae Huh, Ki-Hun Jeong
Summary: The rapid and accurate molecular diagnostics are crucial in the face of pandemic diseases, and the nanoplasmonic PCR provides a new solution for rapid and quantitative molecular diagnostics at point-of-care level.
Article
Chemistry, Analytical
Liang Gong, Siyue Yao, Yidong He, Chengliang Liu
Summary: A new qPCR data analysis method called AERKM is proposed to achieve more accurate results by introducing amplification efficiency (AE) for error correction. It has been verified to have higher precision and stability in 5-point 10-fold gradient qPCR tests of 63 genes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Chemistry, Analytical
Kazuyuki Nobusawa, Huan-Wen Han, Fumie Takei, Ting-Chieh Chu, Noriyasu Hashida, Ichiro Yamashita
Summary: We developed a new electrochemical method for real-time detection of polymerase chain reactions, using a DNA intercalator to enhance charge transfer and monitoring the changes in charge transfer resistance. This simple method has the potential to be a cost-effective and portable PCR device.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
D. Cristiano, M. F. Peruzy, M. Aponte, A. Mancusi, Y. T. R. Proroga, F. Capuano, N. Murru
Summary: The study investigated the detection methods for Yersinia enterocolitica in leafy greens, finding that only ddPCR technology could detect the pathogen in leafy greens contaminated at low levels. Additionally, the incubation time and temperature were shown to significantly impact the survival and concentration of Yersinia enterocolitica in vegetables.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Kazuyuki Nobusawa, Huan-Wen Han, Fumie Takei, Ting-Chieh Chu, Noriyasu Hashida, Ichiro Yamashita
Summary: We developed a new electrochemical impedimetric method for real-time detection of PCR. By adding a specific DNA intercalator and redox mediator, the progress of PCR reaction can be accurately monitored using impedance measurement. This simple method enables cost-effective and portable PCR devices.
ANALYTICAL CHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
R. Meyermans, S. Janssens, A. Cousse, W. Gorssen, X. Hubin, P. Mayeres, W. Veulemans, E. Claerebout, C. Charlier, N. Buys
Summary: The mutation in the myostatin gene in Belgian Blue cattle is associated with susceptibility to psoroptic mange and an increase in lesion size.
Letter
Medicine, General & Internal
Alice V. Stanton, Frederic Leroy, Christopher Elliott, Neil Mann, Patrick Wall, Stefaan De Smet
Article
Multidisciplinary Sciences
Theo A. T. G. van Kempen, Samuel Benitez Punal, Jet Huijser, Stefaan De Smet
Summary: This study compares the absorption efficiency of T-Ac and T in broilers, finding that T-Ac has lower absorption efficiency compared to T, and T can increase tissue levels more effectively.
Article
Agriculture, Dairy & Animal Science
Siavash Salek Ardestani, Mohammad Bagher Zandi, Seyed Milad Vahedi, Steven Janssens
Summary: This study comprehensively investigated the genetic structure and characteristics of Iranian native horse breeds. The researchers employed various genetic analysis methods to compare the genetic structure of Iranian native horses with other horse breeds and identified SNPs and genes associated with different traits. The findings provide valuable insights into the genetic characteristics and population structure of Iranian native horse breeds.
Article
Multidisciplinary Sciences
Wim Gorssen, Carmen Winters, Roel Meyermans, Rudi D'Hooge, Steven Janssens, Nadine Buys
Summary: Pig breeding is undergoing rapid changes due to technological progress and socio-ecological factors. Precision livestock farming technologies are increasingly important for automating phenotyping and achieving breeding goals. However, challenges such as individual identification, data processing, and software availability need to be addressed.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Annatachja De Grande, Richard Ducatelle, Saskia Leleu, Christof Rapp, Cibele Torres, Massimiliano Petracci, Stefaan De Smet, Joris Michiels, Freddy Haesebrouck, Filip Van Immerseel, Evelyne Delezie
Summary: This study evaluated the effect of different zinc sources and vitamin E levels on meat yield and quality in broilers subjected to chronic cyclic heat stress. The results showed that replacing ZnSO4 with ZnAA increased breast meat weight and yield, as well as improved the water-holding capacity of the meat. The study also found that the beneficial effects of ZnAA were independent of the vitamin E level.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
M. Majdeddin, U. Braun, A. Lemme, A. Golian, H. Kermanshahi, S. De Smet, J. Michiels
Summary: Dietary guanidinoacetic acid (GAA) affects creatine (Cr) metabolic pathways, leading to increased cellular Cr and improved broiler performance. However, its impact on oxidative status remains unclear. This study showed that GAA can enhance muscle energy metabolism and indirectly support tolerance against oxidative stress.
Article
Agriculture, Dairy & Animal Science
Bimal Chakkingal Bhaskaran, Roel Meyermans, Wim Gorssen, Gregory Erich Maes, Steven Janssens, Nadine Buys
Summary: This study compared the effects of two euthanasia methods (nitrogen gas and injection) on RNA measurements and the interaction effects with two different tissue storage conditions on RNA measurements. The findings suggest that the nitrogen gas method is a suitable alternative to injection for euthanasia, and RNAlater storage condition can significantly improve RNA integrity.
Article
Chemistry, Analytical
Marilyn De Graeve, Emma Van de Walle, Thomas Van Hecke, Stefaan De Smet, Lynn Vanhaecke, Lieselot Y. Hemeryck
Summary: Although the field of DNA adductomics is still in its infancy, this study evaluated different methods for sample analysis and data processing. The results showed that hydrophilic interaction coupled with high resolution mass spectrometry and thermal acidic hydrolysis in combination with solid-phase extraction provided better results for studying DNA adducts. Furthermore, quality control normalization was found to be more effective compared to other normalization strategies. Therefore, quality control normalization can be recommended as the default data normalization strategy.
ANALYTICA CHIMICA ACTA
(2023)
Article
Agriculture, Dairy & Animal Science
Roel Meyermans, Olivier Heylen, Jessica Bouhuijzen Wenger, Jan Martens, Nadine Buys, Steven Janssens
Summary: Screening for genetic diversity in livestock species breeds is crucial, especially for small populations at risk of extinction. This study used the IMAGE001 genotyping array to evaluate the genetic diversity of the Turkey-headed Malines chicken breed in Belgium. The results showed low genetic diversity in this local breed. This research is the first to use the IMAGE001 array for genotyping a local chicken breed and provides a solid foundation for further evaluation of Belgian chicken heritage.
Article
Agriculture, Dairy & Animal Science
Lea Chapard, Anna Van Thillo, Roel Meyermans, Wim Gorssen, Nadine Buys, Steven Janssens
Summary: In this study, an improved phenotype for show jumping performance was developed, and the importance of including a rider effect in genetic models was assessed. Genetic models based on different performances were compared, and the most suitable one for genetic evaluation was determined.
GENETICS SELECTION EVOLUTION
(2023)
Article
Biotechnology & Applied Microbiology
B. Chakkingal Bhaskaran, R. Meyermans, W. Gorssen, G. E. Maes, J. Buyse, S. Janssens, N. Buys
Summary: The choice of euthanasia method and tissue storage condition can affect gene expression profiles in transcriptomic studies. Nitrogen gas in foam (ANOXIA) is a suitable alternative for euthanizing piglets in transcriptome research. RNAlater (RL) storage preserves RNA better than snap freezing in liquid nitrogen (LN2), but it can also influence differential gene expression in multiple tissues.
Meeting Abstract
Obstetrics & Gynecology
M. Linek, N. Buys, C. von Kleinsorgen, D. Schlembach
GEBURTSHILFE UND FRAUENHEILKUNDE
(2022)