期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 50, 页码 16529-16532出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201809757
关键词
DNA; DNA crystals; DNA nanostructures; DNA nanotechnology; self-assembly
资金
- National Science Foundation [CHE-1708776, EFRI-1332411, CCF-1526650]
- Office of Naval Research [N00014-15-1-2707]
- National Science Foundation of China [81429001]
- Human Frontiers Science Program [RGP0010/2017]
- US Department of Energy (DOE) [DE-SC0007991]
- Gordon and Betty Moore Foundation [GBMF3849]
This manuscript reports a strategy for controlling the crystallization kinetics and improving the quality of engineered self-assembled 3D DNA crystals. Growing large, high-quality biomacromolecule crystals is critically important for determining the 3D structures of biomacromolecules. It often presents a great challenge to structural biologists. Herein, we introduce a rationally designed agent to modulate the crystallization process. Under such conditions, fewer, but larger, crystals that yield diffraction patterns of modestly higher resolution are produced compared with the crystals from conditions without the modulating agent. We attribute the improvement to a smaller number of nuclei and slow growth rate of crystallization. This strategy is expected to be generally applicable for crystallization of other biomacromolecules.
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