Article
Chemistry, Analytical
Farshad Ardakani, Bahram Hemmateenejad
Summary: In this study, a new type of microfluidic paper-based analytical device (μPAD) was developed for the separation of plasma from whole blood without using plasma separation membrane. By controlling the pore size of the paper through lamination and electrolyte addition, the device achieved a longer separation distance for plasma with no significant hemolysis. The device has the potential for multi-assay testing in point-of-care experiments.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Riho Shimazu, Kosuke Tomimuro, Yan Ni, Cristina Malegori, Vahid Hamedpour, Yuki Hiruta, Paolo Oliveri, Maarten Merkx, Daniel Citterio
Summary: This study introduces a new analytical method for point-of-care therapeutic drug monitoring (TDM) of the therapeutic antibody cetuximab using microfluidic thread-based analytical devices. The method integrates various technologies such as pre-treatment, sensing techniques, and image processing, offering a rapid, accurate, and cost-effective approach for individual patients to achieve better treatment outcomes.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Ahmed A. Shalaby, Chia-Wen Tsao, Akihiko Ishida, Masatoshi Maeki, Manabu Tokeshi
Summary: Cancer is a leading cause of death worldwide, and early diagnosis is crucial for successful treatment. In developing countries, there is a need for low-cost, accurate, and easy to use point-of-need (PON) devices for cancer diagnosis and treatment follow-up. Paper-based analytical devices (PADs) are considered a key solution as they provide a low-cost platform for developing PON biosensors. This review discusses the various factors involved in the development of PADs for cancer biomarker detection and summarizes the advantages and disadvantages of each method.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Ayushi Chauhan, Mohet Mittal, Bhushan J. Toley
Summary: This article presents a technique for the in situ synthesis of insoluble analytical reagents in paper-based devices, with a focus on sweat chloride quantification strips. The method of paper stacking enables the uniform rehydration of dried reagents, eliminating the need for hydrophobic barrier patterns and multiple dipping steps. The developed sensor has a wide linear dynamic range and can be used for the diagnosis of cystic fibrosis.
ANALYTICAL METHODS
(2022)
Article
Environmental Sciences
Maria Jesus Gonzalez-Pabon, Eduardo Corton, Federico Figueredo
Summary: This study overcame three primary bottlenecks in the practical use of microbial fuel cell (MFC) analytical devices. By utilizing planktonic bacteria as biorecognition agents, a paper-based MFC toxicity test was developed, showing promising future for rapid and easy-to-use MFC devices in the market.
Review
Chemistry, Analytical
Guozhen He, Tao Dong, Zhaochu Yang, Are Branstad, Lan Huang, Zhuangde Jiang
Summary: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death globally, and the lack of early diagnosis and treatment leads to increased economic burden and medical costs. Decentralized point-of-care diagnostics, such as paper-based analytical devices, offer a cost-effective solution for COPD diagnosis and management, reducing medical expenditures.
Article
Chemistry, Analytical
Hua Zhang, Xiaotong Li, Qunyan Zhu, Zhenxin Wang
Summary: This review provides a comprehensive analysis of paper-based analytical devices (PADs) integrated with electrochemical detection methods. The use of nanomaterials and their composites enhances the performance of electrochemical PADs (ePADs). The review focuses on the fabrication of ePADs, strategies to enhance nanomaterials, and potential applications in environmental monitoring, food safety management, and disease diagnosis. The future perspectives of this research field are also proposed.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Wenliang Li, Xuanye Ma, Yang-Chun Yong, Guozhen Liu, Zhugen Yang
Summary: Detection of pathogens is crucial for global public health, and paper-based microfluidic analytical devices (mu PADs) offer a promising solution for rapid and on-site diagnosis.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Thi Xoan Hoang, Le Minh Tu Phan, Thuy Anh Thu Vo, Sungbo Cho
Summary: Paper-based analytical devices (PADs) are a promising approach for point-of-care detection in biomedical and clinical diagnosis due to their cost-effectiveness, ease of use, and rapid responses. However, their overall sensitivity remains weak, posing a challenge for biosensing scientists. Advances in signal-amplification strategies offer potential for improving PADs in clinical applications.
Article
Medicine, General & Internal
Jaehyung Jeon, Chanyong Park, Dinesh Veeran Ponnuvelu, Sungsu Park
Summary: This study introduces a novel method for fabricating 3D-muPADs with enclosed channels, which show enhanced sensitivity compared to traditional hemi channel designs. The stoppage of evaporation in the enclosed channels significantly improves detection performance, with glucose detection as low as 0.3 mM achieved.
Article
Chemistry, Analytical
Mina Alidoust, Yadollah Yamini, Mahroo Baharfar
Summary: In this study, the applicability of electromembrane extraction (EME) combined with microfluidic paper-based analytical devices (muPADs) was explored. The 3D design of the muPADs allowed for efficient EME and quantitative analysis of copper ions. The proposed platform showed remarkable compatibility with direct analysis from untreated real samples of human blood and spring water.
ANALYTICA CHIMICA ACTA
(2022)
Review
Engineering, Biomedical
Saeed Ebrahimi Fana, Maliheh Paknejad, Mahdi Aminian
Summary: The clinical significance of blood group antigens lies in their ability to trigger immune antibodies that can lead to hemolysis. ABO and D (Rh) are essential for blood transfusions, while rapid and accurate blood grouping methods are crucial in certain clinical situations. Paper-based microfluidics like mu PADs show promise in providing simple, economical, and portable options for rapid blood grouping.
BIOMEDICAL MICRODEVICES
(2021)
Article
Biophysics
Tinglei Ma, Suyue Ren, Yixiang Wang, Haihan Yu, Lin Li, Xu Li, Luqing Zhang, Jinghua Yu, Yan Zhang
Summary: In recent years, point-of-care testing (POCT) technology has shifted from laboratory determination to practical applications. This article highlights the latest advances and principal issues in the design and fabrication of paper-based bipolar electrode electrochemiluminescence (BPE-ECL) sensors widely used in the POCT field. It discusses the attractive physical and chemical properties of cellulose paper, approaches to enhance paper functionality, and the materials used for fabricating paper-based BPE-ECL sensors. The article also introduces methods to enhance BPE-ECL signal and improve detection accuracy, as well as the application of paper-based BPE-ECL sensors in various fields.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Biotechnology & Applied Microbiology
Yuan Jia, Hao Sun, Jinpeng Tian, Qiuming Song, Wenwei Zhang
Summary: Paper-based point-of-care tests have gained interest for their rapid, low-cost, and instrument-free detection capabilities. This review focuses on the development of paper-based POC devices for detecting SARS-CoV-2, discussing principles, performance, advantages, drawbacks, limitations, and prospects of existing devices in the field.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemical Research Methods
Wei Zheng, Kan Wang, Hao Xu, Chujun Zheng, Bo Cao, Qi Qin, Qinghui Jin, Daxiang Cui
Summary: Microfluidic paper-based analytical devices (μPADs) have advantages such as small sample volume, rapid detection rates, low cost, and portability, making them suitable for various applications including food evaluation, disease screening, environmental monitoring, and drug testing. The devices employ detection methods like colorimetry, electrochemistry, chemiluminescence, electrochemiluminescence, and fluorescence-based methodologies. The choice of labeling material and design of microfluidic channels are crucial for detection results, and novel nanocomponents and smart structures have improved device performance.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Mayu Masumoto, Sera Ohta, Misaki Nakagawa, Yuki Hiruta, Daniel Citterio
Summary: This study presents a paper-based bienzymatic colorimetric assay for detecting sarcosine, an important urinary biomarker of prostate cancer, achieving a lower limit of detection and higher selectivity. The presence of polymer was found to restrict the enzyme-driven colorimetric reaction, leading to stronger color development.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Sera Ohta, Ryuya Hiraoka, Yuki Hiruta, Daniel Citterio
Summary: This article introduces a traffic light-like microfluidic paper-based analytical device that allows intuitive semi-quantitative analysis without the need for calibration or subjective interpretation. The device displays assay results as traffic light colors and has been designed to eliminate the risk of misinterpretation. The device relies on sample flow modulation through paperfluidic channels to achieve the desired readout. The platform has been successfully demonstrated for glucose quantification in artificial urine as a proof-of-concept.
Article
Chemistry, Analytical
Riho Shimazu, Kosuke Tomimuro, Yan Ni, Cristina Malegori, Vahid Hamedpour, Yuki Hiruta, Paolo Oliveri, Maarten Merkx, Daniel Citterio
Summary: This study introduces a new analytical method for point-of-care therapeutic drug monitoring (TDM) of the therapeutic antibody cetuximab using microfluidic thread-based analytical devices. The method integrates various technologies such as pre-treatment, sensing techniques, and image processing, offering a rapid, accurate, and cost-effective approach for individual patients to achieve better treatment outcomes.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Kogi Kaizu, Mai Mochida, Hiroaki Imai, Daniel Citterio, Yuki Hiruta
Summary: A highly alkaline stable packing material (CaCO3-PMAcO) has been developed for HPLC to separate basic compounds with high separation efficiency. The particles size and chromatographic conditions were systematically evaluated, and the results showed that the CaCO3-PMAcO column exhibited good separation performance and selectivity for various compounds in alkaline mobile phases.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Polymer Science
Yuki Hiruta
Summary: This review summarizes the recent advancements in the application of stimuli-responsive polymers in the biomedical field, with a focus on the design of PNIPAAm-based polymers and their applications in separation and purification as well as controlled cellular uptake.
Article
Materials Science, Multidisciplinary
Tomoka Yoshii, Mai Mochida, Kogi Kaizu, Yasuki Soda, Kazuyoshi Kanamori, Kazuki Nakanishi, Takaaki Sato, Hiroaki Imai, Daniel Citterio, Yuki Hiruta
Summary: In this study, an alkali-stable column packing material for reversed-phase preparative high-performance liquid chromatography (HPLC) was developed based on eggshells. The material showed excellent separation and stability in HPLC columns after surface treatment and modification.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Materials Science, Multidisciplinary
Kohei Sano, Koki Umemoto, Haruka Miura, Satoshi Ohno, Kyohei Iwata, Rin Kawakami, Masayuki Munekane, Toshihide Yamasaki, Daniel Citterio, Yuki Hiruta, Takahiro Mukai
Summary: Poly[oligo(ethylene glycol) methyl ether methacrylate] (POEGMA) was investigated as a potential drug carrier for tumor diagnosis. Different derivatives of POEGMA with varying molecular weights and side chain lengths were synthesized and labeled with radioisotopes or fluorescence dyes. The results showed that the POEGMA derivatives were efficiently taken up by tumor cells and exhibited high accumulation in tumor tissues. In vivo fluorescence imaging using optimized POEGMA derivatives confirmed their suitability as drug carriers for sensitive tumor diagnosis.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Biochemical Research Methods
Tomoka Yoshii, Akihiro Sakama, Kazuyoshi Kanamori, Kazuki Nakanishi, Hiroaki Imai, Daniel Citterio, Yuki Hiruta
Summary: This article presents a study on an alkali-resistant reversed-phase packing material prepared from eggshells modified with an amphiphilic copolymer for purification of basic drugs. The material showed good selectivity and retention performance, making it effective for purifying alkaline drugs and acid-sensitive compounds.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Hiroki Shigemori, Kento Maejima, Hiroyuki Shibata, Yuki Hiruta, Daniel Citterio
Summary: This study investigated the use of cellophane as an alternative platform for colorimetric assays. Cellophane, a low cost and transparent film, allowed pump-free liquid transport and provided possibilities for simple background coloring, increasing the signal range for colorimetric assays.
Article
Chemistry, Analytical
Mami Ogawa, Aya Katoh, Ryuichi Matsubara, Haruka Kondo, Mizuki Otsuka, Tetsuya Sawatsubashi, Yuki Hiruta, Daniel Citterio
Summary: Silicate ions cause silica scaling in industrial water, and thus their concentrations need to be monitored and controlled at a low level. This work presents a foldable origami-type microfluidic paper-based analytical device for on-site detection of silicate ions using the molybdenum blue reaction. The device showed selectivity, stability, and semi-quantitative analytical performance, making it attractive for industrial monitoring.
ANALYTICAL SCIENCES
(2023)
Article
Polymer Science
Yubuki Nakamura, Marina Nasu, Yutaka Shindo, Kotaro Oka, Daniel Citterio, Yuki Hiruta
Summary: Zwitterionic polymers with net-neutral charge and affinity for cancer cell surfaces exhibit antifouling ability. Six pH-responsive mixed-charge polymers were developed, and the effect of side chain hydrophobicity on their interaction with cancer cell membranes was investigated. The polymers were synthesized using different carboxylic acid monomers and ratios of the neutral spacer moiety, resulting in tunable surface charge switching from net-neutral to positive in response to a weakly acidic tumor environment. The hydrophobicity and pH-responsive nature of these polymers led to pH-selective toxicity, disrupting cell membrane integrity.
Article
Chemistry, Analytical
Rei Kumada, Akihiro Sakama, Yutaka Shindo, Yuzuka Kuronuma, Naoko Iwasawa, Daniel Citterio, Kotaro Oka, Yuki Hiruta
Summary: In this research, the new low-affinity Ca2+ probes KLCA-301 and KLCA-501 were developed for multicolor imaging applications. The evaluation of the fluorescence response indicated the applicability of the KLCA series as low-affinity Ca2+ probes. Furthermore, KLCA-501 achieved simultaneous visualization of Ca2+ and ATP concentrations as well as mitochondrial inner membrane potential in neurons.
ANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Mai Hayakawa, Kosuke Sakano, Rei Kumada, Haruka Tobita, Yasuhiko Igarashi, Daniel Citterio, Yuya Oaki, Yuki Hiruta
Summary: A prediction model for cloud point temperature (T-CP) was developed using a combination of materials informatics and chemical insight. The model can accurately predict the T-CP of polymers containing monomers not included in the dataset, indicating its versatility and potential for guiding the design of new thermo-responsive polymers.
Article
Chemistry, Analytical
Tomoka Yoshii, Kohei Nakano, Tomoaki Okuda, Daniel Citterio, Yuki Hiruta
Summary: Eggshell-based reversed-phase packing materials were used for HPLC analysis. The commercially available eggshell powder was classified into three different particle sizes. Sedimentation separation and packing into analytical columns were performed to evaluate the separation efficiency.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Physical
Taisei Kaku, Koichi Deura, Tomoka Yoshii, Daniel Citterio, Yuki Hiruta
Summary: The aim of this study was to develop a chromatography approach that can switch between cation- and anion-exchange modes by adjusting the monomer ratio. By utilizing a pH-responsive mixed-charge polymer, a single column was able to switch between the two exchange modes. The effectiveness of this method was demonstrated by separating five basic antidepressants and acidic non-steroidal anti-inflammatory drugs.
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2023)
