Article
Materials Science, Paper & Wood
Muhammad Zeeshan Ahmed, Sadaf Mutahir, Azeem Intisar, Nawshad Muhammad, Zeeshan Mutahir
Summary: This study aimed to synthesize probe-sonicated cellulose (PSC) and functionalize it with bone-conditioned medium (BCM) to examine the in silico binding affinity of active components of BCM with cellulose subunits. The in vitro bioactivity of BCM-treated PSC was also investigated, showing favorable biocompatibility and promotion of pre-osteoblast cell proliferation, viability, and mineralization.
Article
Cell Biology
Siddharth Shanbhag, Carina Kampleitner, Niyaz Al-Sharabi, Samih Mohamed-Ahmed, Karol Ali Apaza Alccayhuaman, Patrick Heimel, Stefan Tangl, Andreas Beinlich, Neha Rana, Mariano Sanz, Einar K. Kristoffersen, Kamal Mustafa, Reinhard Gruber
Summary: Functionalizing collagen membranes (MEM) with conditioned media (CM) from human bone marrow MSC (MEM-CM) enhanced new bone formation in rat calvarial defects. Lyophilized MEM-CM (CM-LYO) showed superior radiographic and histological outcomes compared to soaking prepared MEM-CM (CM-SOAK), MEM with rat MSC (CEL), and native MEM groups. Proteomic analysis of CM-LYO revealed enriched proteins and biological processes related to bone formation.
Article
Nanoscience & Nanotechnology
Abdel H. Mahmoud, Yuanyuan Han, Renan Dal-Fabbro, Arwa Daghrery, Jinping Xu, Darnell Kaigler, Sarit B. Bhaduri, Jos Malda, Marco C. Bottino
Summary: In this study, highly innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes were fabricated via electrospinning for guided bone regeneration (GBR). The composite microfibers showed a uniform porous network and successful integration of β-TCP particles. The GelMA/PCL-TCP membranes exhibited enhanced cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs) compared to pure PCL and GelMA/PCL. Furthermore, the GelMA/PCL-TCP membrane promoted robust bone regeneration in rat calvarial critical-size defects, demonstrating remarkable osteogenesis compared to PCL and GelMA/PCL groups. Overall, the electrospun GelMA/PCL-TCP composite showed great potential as a promising membrane for guided bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Solen Novello, Sylvie Tricot-Doleux, Agnes Novella, Pascal Pellen-Mussi, Sylvie Jeanne
Summary: This study evaluated the influence of periodontal ligament stem cell-derived conditioned medium (CM) on osteoblasts and its potential as a therapeutic tool for periodontal regeneration. The findings showed that PDLSC-CM enhanced osteoblast proliferation and increased the expression of some osteoblastic differentiation markers.
Review
Polymer Science
Ali M. Alqahtani
Summary: This comprehensive review provides an in-depth analysis of the use of biomaterials in guided tissue and bone regeneration in dental therapeutic interventions. The review explores different types of membranes and biomaterials used in guided bone regeneration procedures, as well as the manufacturing technologies for the scaffold. The significance of guided bone regeneration and the critical role of membranes in successful bone and tissue regeneration are emphasized.
Article
Materials Science, Multidisciplinary
Zhuyun Cai, Heng Jiang, Tao Lin, Ce Wang, Jun Ma, Rui Gao, Yingying Jiang, Xuhui Zhou
Summary: Microspheres and microsphere-based scaffolds have shown great potential in translational medicine. This review summarizes the various fabrication methods for microspheres, discusses the limitations of existing techniques, and describes the appropriate materials and surface-modification strategies to enhance the biological properties of microspheres. The key application of microsphere-functionalized scaffolds for bone tissue engineering is also discussed.
MATERIALS TODAY ADVANCES
(2022)
Article
Engineering, Biomedical
Zhuo Li, Haixing Wang, Kunyu Zhang, Boguang Yang, Xian Xie, Zhengmeng Yang, Lingchi Kong, Peng Shi, Yuan Zhang, Yi-Ping Ho, Zhi-Yong Zhang, Gang Li, Liming Bian
Summary: The research assembled a biomimetic hyaluronic acid nanocomposite hydrogel with bisphosphonates (BPs), and demonstrated its expedited release of BPs in an acidic environment. The in vitro studies showed that the hydrogel inhibits mature osteoclastic differentiation, while promoting the initial differentiation of primary macrophages to preosteoclasts. In vivo evaluation confirmed the hydrogel's ability to enhance in situ bone regeneration.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Bruno Zwingenberger, Corina Vater, Roland L. Bell, Julia Bolte, Elisabeth Mehnert, Ronny Bruenler, Dilbar Aibibu, Stefan Zwingenberger
Summary: The study found that CHS scaffolds exhibited higher osteogenic properties in vitro, but resulted in lower bone-healing outcomes compared to CBA and MCM in vivo. Future improvements on CHS scaffolds are needed for better mechanical stability and osteoconductivity.
Review
Medicine, Research & Experimental
Hongbing Lin, Huishan Chen, Xuetao Zhao, Zhen Chen, Peipei Zhang, Yue Tian, Yawei Wang, Tong Ding, Lijing Wang, Yuqin Shen
Summary: Periodontitis is a chronic inflammatory disease that poses a challenge for complete regeneration in clinics. Mesenchymal stem cells (MSCs) show potential for regenerating periodontal tissue, but their direct use faces drawbacks such as low survival rate and host immune reactions. MSC culture conditioned medium (CM) offers a promising indirect treatment approach with the potential to overcome these challenges for periodontal tissue regeneration.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Review
Engineering, Biomedical
Guanyin Zhu, Tianxu Zhang, Miao Chen, Ke Yao, Xinqi Huang, Bo Zhang, Yazhen Li, Jun Liu, Yunbing Wang, Zhihe Zhao
Summary: Bone-tissue defects are a global issue, with autologous and allogeneic bone grafting failing to achieve ideal therapeutic effects. This has led researchers to explore novel bone-regeneration methods, with the development of BTE scaffolds at the forefront. By delving into bone physiology and healing mechanisms, biomimicking and bioinspired BTE scaffolds are making progress.
BIOACTIVE MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Fairuz Izan Muhammad Firdaus, Ab. Karim Nashihah, Mh. Busra Mohd Fauzi, Maarof Manira, Saim Aminuddin, Yogeswaran Lokanathan
Summary: In recent years, exploring respiratory therapy guided by cell-secreted substances in vitro has become popular. This review evaluated the efficacy of using conditioned medium (CM) collected in an in vitro respiratory epithelial model. The results showed that differentiated cells from respiratory and non-respiratory systems, such as mesenchymal stem cells (MSC), could improve proliferation, migration, attachment, and protect the respiratory epithelium against cytotoxicity. CM might be a potential regenerative therapy in respiratory illnesses, but further research is needed to justify its use in clinical practice.
APPLIED SCIENCES-BASEL
(2023)
Article
Cell & Tissue Engineering
Batoul Chouaib, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Conditioned medium (CM) produced from dental mesenchymal stem cells (DMSC-CM) is emerging as a potential alternative to cell therapy. However, there is a lack of standardized and optimized manufacturing procedures due to variable protocols, leading to inconsistent results.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Materials Science, Multidisciplinary
Milena S. Malherbi, Luciano C. Dias, Mariana S. Z. Lima, Larissa G. Ribeiro, Valdirlei F. Freitas, Taiana G. M. Bonadio, Ludmilla M. Silva, Gilvan B. Souza, Eduardo A. Volnistem, Jaciele M. Rosso, Daniel M. Silva, Luiz F. Cotica, Wilson R. Weinand, Ivair A. Santos
Summary: This study synthesized hydroxyapatite/beta-tricalcium phosphate/polyvinylidene fluoride biocomposites and used electrical stimuli to improve and accelerate bone regeneration in vitro. The results showed that the growth rate of hydroxyapatite in the biocomposite significantly increased under specific electric field conditions.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2022)
Review
Engineering, Biomedical
Elia Bosch-Rue, Leire Diez-Tercero, Jenifer Olmos Buitrago, Emilio Castro, Roman A. Perez
Summary: Bone has the ability to self-regenerate, but extensive damage can hinder the natural regeneration process. Bone tissue engineering aims to mimic the extracellular matrix and promote the physiological regeneration process by stimulating osteogenesis and angiogenesis.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
V. K. Anupama Devi, Anjaneyulu Udduttula, Amit Kumar Jaiswal
Summary: Biomedical uses for natural polysaccharides of marine origin, including fucoidan derived from brown algae, are gaining popularity. Despite being less researched compared to other biomaterials, fucoidan is more accessible and cost-effective. This review provides an overview of its history, sources, and characteristics, with a focus on its application in bone tissue engineering. The global research status of fucoidan, especially for bone tissue engineering, is examined, making this study a comprehensive manual for understanding its research status.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Norihisa Ichimura, Masahito Fujio, Naoto Toyama, Hirohisa Yamada, Hideharu Hibi
Summary: Temporomandibular joint herniation is a rare condition where the contents of the temporomandibular joint protrude into the external auditory canal. This case report highlights the characteristic clinical and imaging findings of TJH and emphasizes the importance of understanding its pathophysiology for oral and maxillofacial surgeons.
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Noriyuki Yamamoto, Satoshi Yamaguchi, Masaya Nishikawa, Norihisa Ichimura, Go Ohara, Hideharu Hibi
Summary: Lingual artery (LA) aneurysms are rare, often associated with trauma, infection, and surgical intervention. Idiopathic LA aneurysms are even rarer, with only a few reported cases so far. A 55-year-old woman was diagnosed with an idiopathic aneurysm of the lingual artery, which was successfully treated with aneurysmectomy.
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hirohisa Yamada, Norihisa Ichimura, Noriyuki Yamamoto, Yuki Okumura, Masaya Nishikawa, Satoshi Yamaguchi, Shinji Uejima, Hideharu Hibi
Summary: This article reports a rare case of MEC in the sublingual gland with confirmed MAML2 fusion through detailed molecular analysis. No recurrence was observed within three years after the surgical removal.
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Yoshiro Koma, Takehiro Fujimoto, Kiyoshi Sakai, Yukiko Sugimura, Satoshi Yamaguchi, Hideharu Hibi
Summary: In this report, a patient with bilateral ranulas and asymptomatic Sjogren's syndrome was successfully treated with surgery and sclerotherapy. A literature review suggests that this coexistence may not be very rare and may be characterized by a high prevalence of bilateral ranulas.
ORAL SCIENCE INTERNATIONAL
(2022)
Article
Endocrinology & Metabolism
Shintaro Iwama, Tomoko Kobayashi, Yoshinori Yasuda, Takayuki Okuji, Masaaki Ito, Masahiko Ando, Xin Zhou, Ayana Yamagami, Takeshi Onoue, Yohei Kawaguchi, Takashi Miyata, Mariko Sugiyama, Hiroshi Takagi, Daisuke Hagiwara, Hidetaka Suga, Ryoichi Banno, Tetsunari Hase, Masahiro Morise, Keiko Wakahara, Kenji Yokota, Masashi Kato, Naoki Nishio, Chie Tanaka, Kazushi Miyata, Atsushi Ogura, Takanori Ito, Tsunaki Sawada, Tomoya Shimokata, Kaoru Niimi, Fumiharu Ohka, Masatoshi Ishigami, Momokazu Gotoh, Naozumi Hashimoto, Ryuta Saito, Hitoshi Kiyoi, Hiroaki Kajiyama, Yuichi Ando, Hideharu Hibi, Michihiko Sone, Masashi Akiyama, Yasuhiro Kodera, Hiroshi Arima
Summary: The study revealed that the incidence of thyroid immune-related adverse events was high after PD-1/CTLA-4-Abs treatment even in patients negative for ATAs at baseline.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Pharmacology & Pharmacy
Yao Liu, Fumiya Kano, Noboru Hashimoto, Linze Xia, Qiao Zhou, Xingmei Feng, Hideharu Hibi, Aya Miyazaki, Tsutomu Iwamoto, Yoshizo Matsuka, Zhijun Zhang, Eiji Tanaka, Akihito Yamamoto
Summary: This study investigates the therapeutic potential of conditioned medium derived from stem cells from human exfoliated deciduous teeth (SHED-CM) against neuropathic pain (NP). They found that SHED-CM can ameliorate NP by recruiting anti-inflammatory M2 macrophages and suppressing inflammation.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kohei Sakaguchi, Nozomu Taguchi, Rina Kobayashi, Kei Taguchi, Kei Okada, Fumie Kinoshita, Hideharu Hibi
Summary: Exercise therapy has immediate curative effects in patients with masticatory muscle myalgia, enlarging mouth opening distance and reducing pain. There were no instances of pain exacerbation or reduction in mouth opening post-exercise therapy, indicating its safety and efficacy. Pain reduction was more significant in patients with unilateral pain compared to those with bilateral pain.
JOURNAL OF ORAL REHABILITATION
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kiyoshi Sakai, Noriyuki Yamamoto, Satoshi Yamaguchi, Kazuto Okabe, Yoshiro Koma, Hideharu Hibi
Summary: The case report describes a 22-month-old boy with multiple-bone Langerhans cell histiocytosis (LCH). The patient presented with left cheek swelling, and imaging exams revealed bone resorption in the left mandibular angle. Further tests confirmed the diagnosis of LCH, and the patient underwent chemotherapy. After four years of treatment, the patient remains in good health without recurrence.
ORAL SCIENCE INTERNATIONAL
(2023)
Editorial Material
Infectious Diseases
Norihisa Ichimura, Noriyuki Yamamoto, Yusuke Urata, Shohei Ikutomi, Hideharu Hibi
Article
Engineering, Biomedical
Xinman Song, Kazuto Okabe, Yuya Ohta, Go Ohara, Naoto Toyama, Qi Chang, Yilin Wang, Hideharu Hibi
Summary: FAM20B plays a crucial role in osteogenic differentiation and bone formation on titanium surfaces. Depletion of FAM20B inhibits the formation of a PG-rich layer between bone cells and titanium surfaces, reduces MSC osteogenesis signaling pathway, and decreases the expression of osteogenic marker genes.
ACTA BIOMATERIALIA
(2023)
Article
Dentistry, Oral Surgery & Medicine
Go Ohara, Kazuto Okabe, Naoto Toyama, Yuya Ohta, Song Xinman, Norihisa Ichimura, Kotaro Sato, Yusuke Urata, Hideharu Hibi
Summary: It was found that hyperthermia enhances apoptotic signaling by tumor necrosis factor-related apoptosis-inducing ligand via the suppression of death receptor ubiquitination, which upregulates death receptor expression. These results suggest that the combination of hyperthermia and tumor necrosis factor-related apoptosis-inducing ligand may serve as a novel treatment strategy for oral squamous cell carcinoma.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Article
Multidisciplinary Sciences
Fumiya Kano, Noboru Hashimoto, Yao Liu, Linze Xia, Takaaki Nishihara, Wakana Oki, Keita Kawarabayashi, Noriko Mizusawa, Keiko Aota, Takayoshi Sakai, Masayuki Azuma, Hideharu Hibi, Tomonori Iwasaki, Tsutomu Iwamoto, Nobuyasu Horimai, Akihito Yamamoto
Summary: Radiation therapy for head and neck cancers can cause severe radiation-induced xerostomia. Conditioned medium derived from stem cells from human exfoliated deciduous teeth (SHED-CM) was found to prevent ulcer formation and maintain salivary gland function. SHED-CM enhanced antioxidant gene expression and suppressed oxidative stress. The study suggests that SHED-CM may provide substantial therapeutic benefits for radiation-induced xerostomia.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Masahito Fujio, Akira Sayo, Kota Ogisu, Qi Chang, Makoto Tsuboi, Hideharu Hibi
Summary: This study evaluated the stability of Le Fort I osteotomy with zygomatic buttress internal fixation alone. Two groups of patients underwent the procedure, with group I receiving fixation at the piriform aperture and zygomatic buttress, and group II receiving fixation at the zygomatic buttress only. The results showed no significant differences in relapse between the groups, suggesting that the number of plates used for fixation can be determined by the surgeon without compromising outcomes.
NAGOYA JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Kotaro Sato, Kouki Fujii, Hiromasa Tanaka, Masaru Hori, Hideharu Hibi, Shinya Toyokuni
Summary: COVID-19 has been a pandemic since 2020, with the emergence of new variants. The ACE2 receptor and TMPRSS2 play crucial roles in the viral internalization process. This study found abundant expression of ACE2 and TMPRSS2 in the oral cavity, suggesting it may serve as a gateway for COVID-19 infection. The researchers investigated whether tongue vaccination offers any advantages compared to subcutaneous injection.
FREE RADICAL RESEARCH
(2023)
Article
Nutrition & Dietetics
Kotaro Sato, Koki Fujii, Noriyuki Yamamoto, Norihisa Ichimura, Satoshi Yamaguchi, Hirohisa Yamada, Hideharu Hibi, Shinya Toyokuni
Summary: Alcohol intake and female gender are significant risk factors for increasing TMPRSS2 expression, which is an important factor in the entry and amplification of SARS-CoV-2 in the oral cavity.
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2022)
