期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 44, 页码 11803-11807出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201406412
关键词
detergents; membrane proteins; nanodiscs; reaction centers; styrene maleic acid
资金
- Biotechnology and Biological Sciences Research Council of the UK
- Foundation for Fundamental Research on Matter [126]
- Netherlands Organization for Scientific Research (NWO)
- ERC Advanced Investigator [267333]
- BBSRC [BB/I022570/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I022570/1] Funding Source: researchfish
- European Research Council (ERC) [267333] Funding Source: European Research Council (ERC)
Integral membrane proteins often present daunting challenges for biophysical characterization, a fundamental issue being how to select a surfactant that will optimally preserve the individual structure and functional properties of a given membrane protein. Bacterial reaction centers offer a rare opportunity to compare the properties of an integral membrane protein in different artificial lipid/surfactant environments with those in the native bilayer. Here, we demonstrate that reaction centers purified using a styrene maleic acid copolymer remain associated with a complement of native lipids and do not display the modified functional properties that typically result from detergent solubilization. Direct comparisons show that reaction centers are more stable in this copolymer/lipid environment than in a detergent micelle or even in the native membrane, suggesting a promising new route to exploitation of such photovoltaic integral membrane proteins in device applications.
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