期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 46, 期 5, 页码 2096-2106出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2015.2893
关键词
CHIP; migration; invasion; NF-kappa B; TRAF2
类别
资金
- Jiangsu Provincial Natural Science Foundation of China [BK2011306]
- National Natural Science Foundation of China [81172433, 81272737]
Ubiquitin modification of proteins influences cellular processes related to carcinogenesis. The carboxyl terminus of Hsc-70-interacting protein (CHIP), as U-box-type ubiquitin ligase, induces ubiquitination and proteasome-mediated degradation of its substrate proteins. In this study, the role of CHIP in diverse aspects of gastric cancer cells was investigated. CHIP overexpression in the AGS gastric cancer cells caused impaired tumor growth. CHIP overexpression significantly inhibited the migration and invasion of the AGS cells. Moreover, we found that not only RelA/p65 but also Re1B, the NF-kappa B subunits, was negatively regulated by CHIP, likely owing to the TRAF2 reduction. Downregulated target genes of NF-kappa B subunits, including MMP-2 and -9, integrin beta-1 and Bcl-2 were involved in these processes. We also showed that the expression level of CHIP was frequently decreased in gastric cancer tissues and the low level of CHIP expression might be an indicator of an unfavorable prognosis. Taken together, these observations provide functional evidence for CHIP behaviors as a tumor suppressor in gastric cancer.
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