Click-Tag and Amine-Tag: Chemical Tag Approaches for Efficient Protein Labeling In Vitro and on Live Cells using the Naturally SplitNpuDnaE Intein

An Atypical Naturally Split Intein Engineered for Highly Efficient Protein Labeling

(2014) Ilka V. Thiel et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Structure–activity studies on the upstream splice junction of a semisynthetic intein

(2013) Alexandra Wasmuth et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Site-specific protein labeling using PRIME and chelation-assisted click chemistry

(2013) Chayasith Uttamapinant et al.   Nature Protocols

Mutual synergistic protein folding in split intein

(2012) Yuchuan Zheng et al.   BIOSCIENCE REPORTS

Unprecedented Rates and Efficiencies Revealed for New Natural Split Inteins from Metagenomic Sources

(2012) Patricia Carvajal-Vallejos et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Second-Generation Covalent TMP-Tag for Live Cell Imaging

(2012) Zhixing Chen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In-Cell Fluorescence Activation and Labeling of Proteins Mediated by FRET-Quenched Split Inteins

(2012) Radhika Borra et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Circular Permutation Prediction Reveals a Viable Backbone Disconnection for Split Proteins: An Approach in Identifying a New Functional Split Intein

(2012) Yun-Tzai Lee et al.   PLoS One

Kinetic Control of One-Pot Trans-Splicing Reactions by Using a Wild-Type and Designed Split Intein

(2011) Neel H. Shah et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein

(2011) Tulika Dhar et al.   CHEMICAL COMMUNICATIONS

Split Inteins: Nature’s Protein Ligases

(2011) Neel H. Shah et al.   ISRAEL JOURNAL OF CHEMISTRY

Highly Efficient and More Generalcis- andtrans-Splicing Inteins through Sequential Directed Evolution

(2011) Julia H. Appleby-Tagoe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Adding New Chemistries to the Genetic Code

(2010) Chang C. Liu et al.   Annual Review of Biochemistry

How to obtain labeled proteins and what to do with them

(2010) Marlon J Hinner et al.   CURRENT OPINION IN BIOTECHNOLOGY

Protein immobilization on liposomes and lipid-coated nanoparticles by protein trans-splicing

(2010) Nam Ky Chu et al.   JOURNAL OF PEPTIDE SCIENCE

Expanding the Scope of ProteinTrans-Splicing to Fragment Ligation of an Integral Membrane Protein: Towards Modulation of Porin-Based Ion Channels by Chemical Modification

(2009) Steffen Brenzel et al.   CHEMBIOCHEM

Split Inteins as Versatile Tools for Protein Semisynthesis

(2009) Henning D. Mootz   CHEMBIOCHEM

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Novel Split Intein fortrans-Splicing Synthetic Peptide onto C Terminus of Protein

(2009) Julia H. Appleby et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Covalent Protein Labeling Based on Noncatalytic β-Lactamase and a Designed FRET Substrate

(2009) Shin Mizukami et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fractured genes: a novel genomic arrangement involving new split inteins and a new homing endonuclease family

(2009) Bareket Dassa et al.   NUCLEIC ACIDS RESEARCH

In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins

(2009) A. Sesilja Aranko et al.   PLoS One

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology

Site-Specific Chemical Modification of Proteins with a Prelabelled Cysteine Tag Using the Artificially Split Mxe GyrA Intein

(2008) Thomas Kurpiers et al.   CHEMBIOCHEM

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY