Article
Biochemistry & Molecular Biology
Nikita Abramenko, Zdenek Kejik, Robert Kaplanek, Ameneh Tatar, Tereza Brogyanyi, Martina Pajkova, David Sykora, Katerina Vesela, Veronika Antonyova, Petr Dytrych, Masao Ikeda-Saito, Pavel Martasek, Milan Jakubek
Summary: Proton pump inhibitors are important clinically used drugs, but their long-term use may be associated with an increased risk of serious pathological states. The interaction between these drugs and transition metal ions could play a role in their side effects.
CHEMICAL BIOLOGY & DRUG DESIGN
(2021)
Review
Biochemistry & Molecular Biology
Zdenek Kejik, Robert Kaplanek, Michal Masarik, Petr Babula, Adam Matkowski, Petr Filipensky, Katerina Vesela, Jakub Gburek, David Sykora, Pavel Martasek, Milan Jakubek
Summary: Flavonoids, common plant natural products, can suppress ROS-related damage through chelation of transition metal ions, with iron being the most significant. Their interaction with Fe(II) and Fe(III) ions can significantly affect their biological properties. Special biological effects of their iron metallocomplexes, such as the inhibition of iron-containing enzymes, have been discussed in this review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Bara Krizkovska, Rohitesh Kumar, Katerina Rehorova, David Sykora, Simona Dobiasova, Denisa Kucerova, Maria Carmen Tan, Virgilio Linis, Glenn Oyong, Tomas Ruml, Jan Lipov, Jitka Viktorova
Summary: This study compared the composition and biological activities of methanolic and dichloromethane extracts from eight Selaginella species, with flavonoids predominating among the identified 193 compounds. Methanolic extracts showed higher antioxidant capacity and cytotoxicity against cancer cell lines, while some extracts exhibited inhibition of acetylcholinesterase activity. Particularly, Selaginella apoda showed promising potential for further exploration.
Article
Genetics & Heredity
Veronika Prazlenkova, Jiri Funda, Zdenko Pirnik, Alena Karnosova, Lucie Hruba, Lucia Korinkova, Barbora Neprasova, Petra Janovska, Michal Benzce, Michaela Kadlecova, Jaroslav Blahos, Jan Kopecky, Blanka Zelezna, Jaroslav Kunes, Kristina Bardova, Lenka Maletinska
Summary: Deletion of GPR10 gene resulted in changes in lipid metabolism in mice of both sexes, however in different extent. The increase in adipose tissue mass observed in only GPR10 KO males may have been prevented in GPR10 KO females owing to a compensatory increase in the expression of metabolic genes.
Article
Peripheral Vascular Disease
Josef Zicha, Michal Behuliak, Anna Vavrinova, Zdenka Dobesova, Jaroslav Kunes, Hana Rauchova, Ivana Vaneckova
Summary: Salt hypertensive Dahl rats show increased blood pressure response to fasudil, possibly due to augmented calcium sensitization and decreased baroreflex efficiency. In addition, sympathetic and NO interventions, as well as reduced sympathetic compensation, can affect the blood pressure response.
HYPERTENSION RESEARCH
(2021)
Article
Clinical Neurology
Anna Mengr, Lucie Hruba, Aneta Exnerova, Martina Holubova, Andrea Popelova, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: The study showed that palm(11)-PrRP31 has neuroprotective and anti-inflammatory effects in APP/PS1 mice, reducing A beta plaque load and microgliosis, and promoting synaptogenesis.
CURRENT ALZHEIMER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Alena Karnosova, Veronika Strnadova, Lucie Hola, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: Lipidization of PrRP stabilizes the peptide and increases its affinity for anorexigenic receptors GPR10 and NPFF-R2. Two palmitoylated PrRP31 analogs showed high affinity for NPFF-R1 and increased phosphorylation of ERK, Akt, and CREB in receptor-expressing cells. Palm(11)-PrRP31 exhibited fewer off-target activities and has potential as an anti-obesity drug.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Miroslava Kacirova, Blanka Zelezna, Michaela Blazkova, Martina Holubova, Andrea Popelova, Jaroslav Kunes, Blanka Sediva, Lenka Maletinska
Summary: This study found that obesity may lead to neuroinflammation and peripheral insulin resistance in THY-Tau22 mice, potentially exacerbating neurodegenerative diseases, with significant sex differences in neurodegenerative signs.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Physiology
Zdenko Pirnik, Lucia Korinkova, Jana Osacka, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: This study suggests that the CCK receptor and PrRP neuronal system play important roles in the central anorexigenic effect. The exogenously influenced CCK system may be involved in the central anorexigenic effect of peripherally applied palm-PrRP31.
PHYSIOLOGICAL RESEARCH
(2021)
Review
Pharmacology & Pharmacy
Lucia Mrazikova, Barbora Neprasova, Anna Mengr, Andrea Popelova, Veronika Strnadova, Lucie Hola, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: The review highlights obesity and type 2 diabetes as preconditions for metabolic syndrome, while suggesting that anorexigenic peptides like PrRP could be a promising tool for the treatment of obesity and related metabolic complications. Specifically, the study on palmitoylated PrRP31 analogs showed potential therapeutic effects on food intake and body weight, dependent on intact leptin signaling, indicating a possible treatment avenue for obesity and metabolic complications.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Endocrinology & Metabolism
Lucia Mrazikova, Silvie Hojna, Andrea Pacesova, Lucie Hruba, Veronika Strnadova, Barbora Neprasova, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: This study found that palm(11)-PrRP31 has neuroprotective properties in fa/fa rat models with leptin signaling disruption, but does not have anti-obesity and glucose-lowering effects.
NUTRITION & DIABETES
(2022)
Article
Cell Biology
Andrea Pacesova, Martina Holubova, Lucie Hruba, Veronika Strnadova, Barbora Neprasova, Helena Pelantova, Marek Kuzma, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: Ageing is the most important risk factor for the development of sporadic Alzheimer's disease (AD). In this study, specific changes in neurodegenerative processes were observed between the 3rd and 6th month of age in SAMP8 mice, providing a potential therapeutic window for neuroprotective interventions.
Article
Nutrition & Dietetics
Lucia Mrazikova, Silvie Hojna, Petra Vaculova, Stepan Strnad, Vladimir Vrkoslav, Helena Pelantova, Marek Kuzma, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: A stable analog of Prolactin-releasing peptide (PrRP), called palm(11)-PrRP31, has been developed and shown to have anti-obesity and glucose-lowering effects in animals with high-fat diet-induced obesity. In addition, it was found to attenuate neuroinflammation in the brain. This study suggests potential therapeutic benefits of palm(11)-PrRP31 and liraglutide in treating obesity and its related complications.
Article
Pharmacology & Pharmacy
Leandro Ceotto Freitas-Lima, Andrea Pacesova, Jana Stanurova, Pavel Sacha, Ales Marek, Martin Hubalek, Jaroslav Kunes, Blanka Zelezna, Lenka Maletinska
Summary: The study found that GPR160 is not the receptor for CARTp, as no specific binding of CARTp was detected in PC12 cells, THP1 cells, and GPR160-transfected cells.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Endocrinology & Metabolism
Veronika Strnadova, Alena Karnosova, Miroslava Blechova, Barbora Neprasova, Lucie Hola, Anna Nemcova, Aneta Myskova, David Sykora, Blanka Zelezna, Jaroslav Kunes, Lenka Maletinska
Summary: Researchers designed new lipidized PrRP31 analogs with different lengths of fatty acids attached at amino acids 1 or 11 directly or via linkers. These analogs showed strong affinity for GPR10 and NPFF-R2 receptors and effectively reduced body weight in mice with diet-induced obesity.