Article
Chemistry, Physical
Sophie Wintermans, Jordi F. Keijzer, Marte Dros, Han Zuilhof, Bauke Albada
Summary: The aptamer-functionalized hGQ DNAzymes enhance the bioconjugation of N-methyl luminol to tyrosine-containing residues and peptides, with a 12-fold increase in the catalytic rate constant. When applied for modifying Tyr-containing peptides, the nucleoapzymes not only recognize the ligand structure embedded in a larger peptide structure, but also show that distant residues in the peptide substrate can influence the conversion.
Article
Biochemistry & Molecular Biology
Aimee J. Horsfall, Daniel P. McDougal, Denis B. Scanlon, John B. Bruning, Andrew D. Abell
Summary: The introduction of a bimane-containing linker into a peptide targeting Estrogen Receptor alpha was shown to stabilize alpha-helical geometry, with the constrained peptide adopting a 3(10)-helical structure in solution. The study also revealed that the bimane modification influences peptide structure in a sequence dependent manner, with fluorescence intensity of the bimane being influenced by peptide conformation.
Article
Chemistry, Physical
Antonia Kowalewski, Nancy R. Forde, Chapin S. Korosec
Summary: This study presents a model for multivalent diffusive transport and derives an analytical expression for the diffusion coefficient of a general multivalent diffusive process based on simulation results. The findings can provide guidance for experimental design of multivalent transporters, particularly in overcoming trade-offs between diffusivity and processivity.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Multidisciplinary
Zichen Liu, Irene Lepori, Mahendra D. Chordia, Brianna E. Dalesandro, Taijie Guo, Jiajia Dong, M. Sloan Siegrist, Marcos M. Pires
Summary: A novel assay combining metabolic tagging, click chemistry, and fluorescent labeling was developed to measure the permeation of small molecules in Mycobacterium tuberculosis. The assay proved to be robust and compatible with high-throughput analysis, providing insights into drug uptake in mycobacteria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Maximilian Buttner, Christoffer B. Lagerholm, Dominic Waithe, Silvia Galiani, Wolfgang Schliebs, Ralf Erdmann, Christian Eggeling, Katharina Reglinski
Summary: Expansion microscopy (ExM) enhances spatial resolution for imaging tissues by crosslinking proteins to a swellable gel, allowing hidden subcellular structures to be resolved. Variability in expansion factors among cellular compartments highlights the need for thorough validation of this technique, with potential quantification methods and challenges discussed.
Article
Biochemical Research Methods
Brant D. Gates, Jackson B. Vyletel, Lei Zou, Matthew J. Webber
Summary: Certain supramolecular motifs, such as Cucurbit[7]uril (CB[7]) macrocycles, exhibit affinities comparable to well-known biological interactions. By modifying cells with CB[7] constructed from a polyamidoamine (PAMAM) dendrimer scaffold, guest-linked drugs can be delivered to the cell membrane, offering potential applications in biomedicine. This approach has the potential to be used for in situ imaging, modulation of cell-based therapies, or the construction of synthetic supramolecular recognition axis on the cell membrane.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ivan Gallego, Javier Ramos-Soriano, Alejandro Mendez-Ardoy, Justo Cabrera-Gonzalez, Irene Lostale-Seijo, Beatriz M. Illescas, Jose J. Reina, Nazario Martin, Javier Montenegro
Summary: Fully substituted peptide/[60]fullerene hexakis-adducts have the potential for multivalent protein recognition and offer synthetically simpler alternatives to natural ligands.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Chemistry, Multidisciplinary
Nico J. Overeem, Erhard van der Vries, Jurriaan Huskens
Summary: This review explores how influenza viruses navigate mucus and recognize host cells through multivalent interactions. The surface proteins of the influenza A virus facilitate specific binding to host cells, and the importance of these multivalent interactions in crossing mucus and entering host cells is discussed. Techniques to measure the avidity and receptor specificity of influenza viruses are also reviewed.
Article
Medicine, Research & Experimental
Oleksandr Zavoiura, Bodo Brunner, Peter Casteels, Luciana Zimmermann, Matthias Ozog, Carlo Boutton, Mike W. Helms, Timothy Wagenaar, Volker Adam, Josefine Peterka, Christiane Metz-Weidmann, Pieter Deschaght, Sabine Scheidler, Kerstin Jahn-Hofmann
Summary: Targeted delivery of siRNA to specific cells remains challenging, but using anti-EGFR Nanobodies for delivery shows promise. The siRNA-conjugated Nanobodies retain their binding and activity in EGFR-positive cells, inducing mRNA cleavage. Comparing different linkers for siRNA conjugation provides insights into improving Nanobody-mediated siRNA delivery.
MOLECULAR PHARMACEUTICS
(2021)
Article
Chemistry, Multidisciplinary
Weifeng Lin, Louis P. Conway, Miroslav Vujasinovic, J-Matthias Lohr, Daniel Globisch
Summary: The study developed a new chemical biology tool to overcome analytical challenges in metabolite analysis, enabling detection of over 200 metabolites in human fecal, urine, and plasma samples. This comprehensive mass spectrometric analysis enhances metabolomics-driven biomarker discovery.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Organic
Vikram Thimaradka, Hayata Utsunomiya, Tomonori Tamura, Itaru Hamachi
Summary: The His-tag/NiNTA interaction promotes a new catalytic protein acylation strategy, enabling selective protein labeling in biological milieu. The recognition-assisted activation mechanism allows for efficient protein labeling at lower substrate concentrations, enabling highly selective and efficient cell-surface receptor modification in live cells.
Article
Chemistry, Physical
C. Wu, H. Tan, W. Huang, C. Liu, W. Wei, L. Chen, Q. Yan
Summary: Aquous multivalent metal-ion batteries (AMMIBs) have been widely used in wearable devices, consumer electronics, and electric vehicles due to their unique characteristics. Layered-structure materials are considered promising electrode materials for AMMIBs, but face challenges such as dissolution, structural instability, low conductivity, and poor electrochemical properties. Various strategies are discussed in detail to address these issues and provide insights for the design and optimization of high-performance AMMIBs, focusing on morphology, cathode structure, and electrolyte. Suggestions for future research direction in this field are provided as well.
MATERIALS TODAY ENERGY
(2021)
Review
Biochemical Research Methods
Zhihui Liang, Yuan He, Christina S. U. Ieong, Chung Hang Jonathan Choi
Summary: This review provides insights into the interactions between Polydopamine nanoparticles (PDA NPs) and cells. The study reveals that PDA NPs can self-target cells through dopamine receptors without the need for ligands, and can exhibit self-therapeutic cellular responses in the absence of drugs. The review also discusses the cellular localization and pharmacological properties of PDA NPs upon various routes of administration.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jake H. H. Schwab, Jake B. B. Bailey, Milan Gembicky, Julia M. M. Stauber
Summary: Multivalency is important in nature for strong and reversible interactions, and its design in synthetic assemblies is of interest to chemists for understanding biological recognition and creating chemical tools. This study presents a systematic and modular approach to design molecular multivalent protein-binding constructs, allowing for control over size, morphology, and valency. The assemblies showed strong multivalent binding to the model lectin Concanavalin A, with the strength of binding depending on the number of saccharide units present.
Review
Biochemistry & Molecular Biology
Vincent Joseph, Mindy Levine
Summary: The article reviews key research accomplishments of Professor Ronald Charles D. Breslow throughout his more than 60 year research career, covering a wide range of topics including physical organic chemistry, medicinal chemistry, bioorganic chemistry, molecular electronics, and origin of chirality. Breslow's contributions to the broader chemistry profession are also briefly reviewed, including his work for the American Chemical Society and gender equity promotion.
BIOORGANIC CHEMISTRY
(2021)
Meeting Abstract
Cardiac & Cardiovascular Systems
David Siu, Chun Ka Wong, Pak Hei Chan, Yuk Kee Poh, Ming-Zher Poh
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2016)
Article
Nanoscience & Nanotechnology
David Alsteens, Richard Newton, Rajib Schubert, David Martinez-Martin, Martin Delguste, Botond Roska, Daniel J. Mueller
NATURE NANOTECHNOLOGY
(2017)
Article
Multidisciplinary Sciences
Martin Kragl, Rajib Schubert, Haiko Karsjens, Silke Otter, Barbara Bartosinska, Kay Jeruschke, Juergen Weiss, Chunguang Chen, David Alsteens, Oliver Kuss, Stephan Speier, Daniel Eberhard, Daniel J. Mueller, Eckhard Lammert
NATURE COMMUNICATIONS
(2016)
Article
Multidisciplinary Sciences
George Eng, Benjamin W. Lee, Lev Protas, Mark Gagliardi, Kristy Brown, Robert S. Kass, Gordon Keller, Richard B. Robinson, Gordana Vunjak-Novakovic
NATURE COMMUNICATIONS
(2016)
Article
Medicine, Research & Experimental
Aranzazu Villasante, Alessandro Marturano-Kruik, Srikanth R. Ambati, Zen Liu, Amandine Godier-Furnemont, Hesam Parsa, Benjamin W. Lee, Malcolm A. S. Moore, Gordana Vunjak-Novakovic
Article
Engineering, Biomedical
Benjamin W. Lee, Bohao Liu, Adam Pluchinsky, Nathan Kim, George Eng, Gordana Vunjak-Novakovic
ADVANCED HEALTHCARE MATERIALS
(2016)
Article
Cardiac & Cardiovascular Systems
Xiangchong Wang, He Tang, Eric Q. Wei, Zhihua Wang, Jing Yang, Rong Yang, Sheng Wang, Yongjian Zhang, Geoffrey S. Pitt, Hailin Zhang, Chuan Wang
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Rajib Schubert, Stuart Trenholm, Kamill Balint, Georg Kosche, Cameron S. Cowan, Manuel A. Mohr, Martin Munz, David Martinez-Martin, Gotthold Flaschner, Richard Newton, Jacek Krol, Brigitte Gross Scherf, Keisuke Yonehara, Adrian Wertz, Aaron Ponti, Alexander Ghanem, Daniel Hillier, Karl-Klaus Conzelmann, Daniel J. Muller, Botond Roska
NATURE BIOTECHNOLOGY
(2018)
Article
Multidisciplinary Sciences
Eric Q. Wei, Daniel S. Sinden, Lan Mao, Hailin Zhang, Chuan Wang, Geoffrey S. Pitt
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Engineering, Biomedical
Bohao Liu, Benjamin W. Lee, Koki Nakanishi, Aranzazu Villasante, Rebecca Williamson, Jordan Metz, Jinho Kim, Mariko Kanai, Lynn Bi, Kristy Brown, Gilbert Di Paolo, Shunichi Homma, Peter A. Sims, Veli K. Topkara, Gordana Vunjak-Novakovic
NATURE BIOMEDICAL ENGINEERING
(2018)
Article
Biochemistry & Molecular Biology
Rajib Schubert, Nico Strohmeyer, Mitasha Bharadwaj, Subramanian P. Ramanathan, Michael Krieg, Jens Friedrichs, Clemens M. Franz, Daniel J. Muller
Article
Biology
Stefan Brueckner, Rajib Schubert, Timo Kraushaar, Raimo Hartmann, Daniel Hoffmann, Eric Jelli, Knut Drescher, Daniel J. Mueller, Lars Oliver Essen, Hans-Ulrich Moesch
Article
Multidisciplinary Sciences
Sheena N. Smith, Rajib Schubert, Branko Simic, Dominik Brucher, Markus Schmid, Niels Kirk, Patrick C. Freitag, Viviana Gradinaru, Andreas Pluckthun
Summary: The research introduces a novel gene therapy platform that enables localized treatment within tumors, increasing both the specificity of antibody transduction and the production of antibodies. It demonstrates that this approach significantly reduces the systemic toxicity of drugs while enhancing therapeutic efficacy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Rajib Schubert, Taegeun Bae, Branko Simic, Sheena N. Smith, Seong-Ho Park, Gabriela Nagy-Davidescu, Viviana Gradinaru, Andreas Pluckthun, Junho K. Hur
Summary: Tissue clearing combined with deep imaging is a powerful tool for expanding classical histological techniques. However, imaging highly melanin-rich tissues remains challenging. To address this, researchers developed a CRISPR-based gene editing method called CRISPR-Clear, which can be incorporated into existing tissue-clearing workflows. The method was successfully applied to melanoma tumors, allowing detailed characterization through staining and imaging techniques.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Benjamin W. Lee, Matthew A. Caporizzo, Christina Y. Chen, Kenneth C. Bedi Jr, Eliot G. Peyster, Benjamin L. Prosser, Kenneth B. Margulies, Alexia Vite
Summary: This study investigates the properties of heart tissue and cardiomyocytes in osteogenesis imperfecta (OI). The findings suggest that OI cardiomyocytes adapt to low stiffness but exhibit impaired response under pathological stiffness, potentially indicating compromised stress response.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
