Article
Biochemistry & Molecular Biology
Xiaohong Yuan, Yong Wu, Jia Lin, Qinyong Weng, Linqing Wu, Shu Yang, Xin Li, Ming Tan, Zhenxing Lin, Xiaolan Lian, Yuanzhong Chen
Summary: This study investigated the value of plasma fibronectin (pFN) in the diagnosis and prognosis of sepsis and found that pFN can more accurately reflect the severity and prognosis of advanced sepsis. The study also revealed that the absence of pFN alters the cytokine storm and phagocytic function of macrophages.
Review
Immunology
Yugo Morita, Elysia A. Masters, Edward M. Schwarz, Gowrishankar Muthukrishnan
Summary: IL-27 is a complex cytokine with both pro-inflammatory and immunosuppressive effects, making its exact role in infectious diseases unclear and in need of further research. Furthermore, as interest grows in managing acute and chronic infections, as well as severe cytokine storms from sepsis, understanding IL-27's dual functions could lead to therapeutic strategies to improve patient outcomes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Yue Liu, Kai Fu, Eric M. Wier, Yifan Lei, Andrea Hodgson, Dongqing Xu, Xue Xia, Dandan Zheng, Hua Ding, Cynthia L. Sears, Jian Yang, Fengyi Wan
Summary: This study identified a novel genotoxin called UshA in attaching/effacing pathogens, which triggers DNA damage and initiates tumorigenic transformation during bacterial infections. Furthermore, it was found that UshA plays a critical role in accelerating colon tumorigenesis in mice. These findings highlight the importance of UshA in the development of colon cancer caused by bacterial infections.
Article
Biochemistry & Molecular Biology
Philip A. Efron, Dijoia B. Darden, Zhongkai Wang, Dina C. Nacionales, Maria-Cecilia Lopez, Russell B. Hawkins, Michael C. Cox, Jaimar C. Rincon, Ricardo Ungaro, Marvin L. Dirain, Gabriela L. Ghita, Tianmeng Chen, Timothy R. Billiar, Matthew J. Delano, Christiaan Leeuwenburgh, Azra Bihorac, Scott C. Brakenridge, Frederick A. Moore, Alicia M. Mohr, Ronald G. Tompkins, Babette A. Brumback, Henry Baker, Gilbert R. Upchurch, Lyle L. Moldawer
Summary: The improved murine model of surgical sepsis more closely approximates human surgical sepsis patients in terms of gene expression, particularly in the more chronic phases of sepsis.
Article
Pharmacology & Pharmacy
Hideaki Yamada, Naoaki Sakata, Tomoko Tanaka, Hideaki Tagashira, Gumpei Yoshimatsu, Ryo Kawakami, Hideichi Wada, Takahiro Iwamoto, Shohta Kodama
Summary: This study showed that activating TRPV4 can improve blood flow in the ischemic hindlimb animal model by promoting both angiogenesis and lymphangiogenesis. It suggests that upregulating both lymphatic and blood vessels through TRPV4 activation could be a promising therapeutic strategy for peripheral artery disease.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2021)
Article
Critical Care Medicine
Zhanqin Zhang, Chaoying Yan, Jiwen Miao, Kairui Pu, Hongli Ma, Qiang Wang
Summary: The study showed that mitochondrial transplantation can reduce systemic inflammation and organ injury, enhance bacterial clearance, and improve survival rate in sepsis. Microarray analysis revealed significant changes in gene expression related to inflammatory response, complement and coagulation cascades, and rejection reaction in polymicrobial sepsis.
Article
Multidisciplinary Sciences
Daniel E. Leisman, Jamie R. Privratsky, Jake R. Lehman, Mabel N. Abraham, Omar Y. Yaipan, Mariana R. Brewer, Ana Nedeljkovic-Kurepa, Christine C. Capone, Tiago D. Fernandes, Robert Griffiths, William J. Stein, Marcia B. Goldberg, Steven D. Crowley, Rinaldo Bellomo, Clifford S. Deutschman, Matthew D. Taylor
Summary: Angiotensin-II treatment in sepsis can enhance the innate immune response and reduce bacterial infection, making it a potential treatment to augment immune responses in sepsis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Weikang Wang, Ding Xu, Peng Luo, Yulong Shi, Thomas Tschernig, Johannes Greven, Frank Hildebrand, Klemens Horst
Summary: Trauma hemorrhage and subsequent sepsis can cause severe organ damage, especially in the liver. However, administration of MALP-2 was found to reduce hepatic immune response and tissue damage in a two-hit model, with the most pronounced effects when applied at the end of trauma hemorrhage.
Article
Cell & Tissue Engineering
M. K. M. Kim, R. Ramachandran, C. A. Seguin
Summary: The study characterized the expression and function of the mechano-sensitive ion channel TRPV4 in intervertebral disc (IVD) cells. TRPV4 was found to play an important role in regulating IVD mechano-biology, including Ca2+ signaling, actin cytoskeleton remodeling, and gene expression changes in response to mechanical stimulation.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Cell & Tissue Engineering
Joshua T. Cohen, Michael Danise, Jason T. Machan, Runping Zhao, Craig T. Lefort
Summary: Transplantation of NPs was found to decrease the expression of inhibitory factors in host neutrophils and reduce proinflammatory cytokines in the lungs after shock, increasing neutrophil migration and enhancing bacterial clearance, indicating the potential of NPs as a cellular therapy for secondary infection following hemorrhagic shock.
Article
Immunology
Lechang Yu, Ying Liu, Chao Cao, Liheng Yang, Haijing Liu, Chunli Wang
Summary: Andrographolide (AD) can improve the survival of mice with intra-abdominal sepsis by enhancing bacterial clearance through increasing the phagocytic activity of macrophages.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Article
Medicine, General & Internal
Algirdas Dagys, Goda Laucaityte, Augusta Volkeviciute, Silvijus Abramavicius, Rimantas Kevalas, Astra Vitkauskiene, Lina Jankauskaite
Summary: This study evaluates the role of certain cytokines and chemokines in the diagnosis of bacterial infection and sepsis in the pediatric emergency department. The results show that IL-2 is the most specific biomarker for identifying bacterial infection, and combining IL-2, IL-6, CRP, WBC, and neutrophil count improves diagnostic ability. Furthermore, IL-10 exhibits high specificity in recognizing sepsis in the early hours of disease onset.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Biology
Mairene Coto-Llerena, Marco Lepore, Julian Spagnuolo, Daniela Di Blasi, Diego Calabrese, Aleksei Suslov, Glenn Bantug, Francois Ht Duong, Luigi M. Terracciano, Gennaro De Libero, Markus H. Heim
Summary: IFNλ plays a crucial role in innate pathogen defense in the lung and intestine, with IFNλ4 inhibiting spontaneous clearance of HCV and affecting T-cell responses. However, the mechanism by which IFNλ4 promotes CD8(+) T-cell responses and inhibits host immunity to HCV infections remains unclear.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemistry & Molecular Biology
Stephanie J. Wan, Ananya Datta, Orneika Flandrin, Matteo M. E. Metruccio, Sophia Ma, Vincent Nieto, Abby R. Kroken, Rose Z. Hill, Diana M. Bautista, David J. Evans, Suzanne M. J. Fleiszig
Summary: The study revealed that corneal nerves and TRPA1/TRPV1 play important roles in preventing bacterial adhesion in the eye by supporting immune cells and maintaining epithelial barrier function to protect the cornea from bacterial invasion.
Article
Cell Biology
Brittney Williams, Jing Zhu, Lin Zou, Wei Chao
Summary: This study demonstrates the pivotal role of TLR7 signaling in platelet activation and platelet-leukocyte aggregate formation during bacterial sepsis.
Article
Clinical Neurology
Anthony F. Domenichiello, Matthew R. Sapio, Amelia J. Loydpierson, Dragan Maric, Taichi Goto, Mark S. Horowitz, Gregory S. Keyes, Zhi-Xin Yuan, Sharon. F. Majchrzak-Hong, Andrew J. Mannes, Michael J. Iadarola, Christopher E. Ramsden
Summary: Oxylipins, lipid peroxidation products, play a role in nociceptive, inflammatory, and vascular responses. Their effects depend on tissue-specific accumulation of polyunsaturated fatty acid precursors and enzyme expression. Technical challenges in studying oxylipins in nociception have led to gaps in knowledge, requiring further research.
Review
Medicine, Research & Experimental
Lillian Hallmark, Luis E. F. Almeida, Sayuri Kamimura, Meghann Smith, Zenaide M. N. Quezado
Summary: Sickle cell disease patients suffer from complex pain issues, where nitric oxide may play a significant role. However, there is currently controversy over the hypothesis of its role in acute pain crises in sickle cell disease.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Hematology
Laxminath Tumburu, Shohini Ghosh-Choudhary, Fayaz T. Seifuddin, Emilia A. Barbu, Simon Yang, Maliha M. Ahmad, Lauren H. W. Wilkins, Ilker Tunc, Ishwarya Sivakumar, James S. Nichols, Pradeep K. Dagur, Shutong Yang, Luis E. F. Almeida, Zenaide M. N. Quezado, Christian A. Combs, Eric Lindberg, Christopher K. E. Bleck, Jun Zhu, Arun S. Shet, Jay H. Chung, Mehdi Pirooznia, Swee Lay Thein
Summary: Sickle cell disease is driven by chronic inflammation associated with elevated cell-free DNA, specifically mitochondrial DNA, in patients. The abnormal retention of mitochondria in circulating red blood cells of patients contributes to the increased levels of cf-mtDNA. High levels of cf-mtDNA in patient plasma trigger the formation of neutrophil extracellular traps, implicating activation of the cGAS-STING pathway.
Article
Hematology
Alfia Khaibullina, Luis E. F. Almeida, Sayuri Kamimura, Patricia M. Zerfas, Meghann L. Smith, Sebastian Vogel, Paul Wakim, Olavo M. Vasconcelos, Martha M. Quezado, Iren Horkayne-Szakaly, Zenaide M. N. Quezado
Summary: Even without strokes, patients with sickle cell disease may suffer from significant neurocognitive deficits, possibly due to mechanisms unrelated to major cerebrovascular problems. Increased cerebral oxidative stress and stress-induced tissue damage in susceptible brain regions may contribute to neurocognitive deficits in SCD mice.
BLOOD CELLS MOLECULES AND DISEASES
(2021)
Article
Clinical Neurology
Taichi Goto, Matthew R. Sapio, Dragan Maric, Jeffrey M. Robinson, Leorey N. Saligan, Andrew J. Mannes, Michael J. Iadarola
Summary: This study investigated the molecular dynamics and transcriptional profiles of inflammation and pain in a rat carrageenan-induced peripheral inflammation model, revealing a temporally orchestrated process of molecular and cellular recruitment. The results identified potential targets for modulation of pain and inflammation, providing foundational data for identifying markers and potential treatments for inflammation and pain in patients.
Article
Biochemistry & Molecular Biology
Sebastian Vogel, Sayuri Kamimura, Taruna Arora, Meghann L. Smith, Luis E. F. Almeida, Christian A. Combs, Swee Lay Thein, Zenaide M. N. Quezado
Summary: The NLRP3 inflammasome is upregulated in sickle cell disease and is dependent on BTK. Pharmacological inhibition of NLRP3 and BTK can decrease platelet aggregation and in vitro thrombus formation in SCD mice. Targeting the NLRP3 inflammasome may offer a novel approach for antiplatelet therapy in SCD.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Clinical Neurology
Matthew R. Sapio, Jenny J. Kim, Amelia J. Loydpierson, Dragan Maric, Taichi Goto, Fernando A. Vazquez, Mary K. Dougherty, Radhika Narasimhan, Wallis T. Muhly, Michael J. Iadarola, Andrew J. Mannes
Summary: This study investigates molecular modulatory processes in the dorsal spinal cord during persistent pain, revealing specific neuronal gene regulation and broad transcriptional signatures for tissue remodeling, synaptic rearrangement, and immune signaling. The research aims to identify non-opioid analgesic targets for addressing the opioid crisis and meeting unmet clinical needs.
Article
Biochemistry & Molecular Biology
Taichi Goto, Matthew R. Sapio, Dragan Maric, Jeffrey M. Robinson, Anthony F. Domenichiello, Leorey N. Saligan, Andrew J. Mannes, Michael J. Iadarola
Summary: The study focused on investigating temporal changes in behavior, tissue structure, and transcriptomic profiles in a rat model of a surgical incision, revealing potential biomarkers or therapeutic targets for wound pain and wound healing. The upregulation of specific peptidases during the late phase of wound healing supports the idea of sustained tissue remodeling process. The study provides important foundational knowledge to understand mechanisms of wound pain and wound healing by examining longitudinal temporal molecular responses, corresponding anatomical localization, and hyperalgesic behavioral alterations.
Article
Medicine, General & Internal
Christopher E. Ramsden, Daisy Zamora, Keturah R. Faurot, Beth MacIntosh, Mark Horowitz, Gregory S. Keyes, Zhi-Xin Yuan, Vanessa Miller, Chanee Lynch, Gilson Honvoh, Jinyoung Park, Russell Levy, Anthony F. Domenichiello, Angela Johnston, Sharon Majchrzak-Hong, Joseph R. Hibbeln, David A. Barrow, James Loewke, John M. Davis, Andrew Mannes, Olafur S. Palsson, Chirayath M. Suchindran, Susan A. Gaylord, J. Douglas Mann
Summary: This study aimed to investigate the effects of dietary interventions on lipid mediators involved in headache pathogenesis and headache frequency in adults with migraines. Results showed that increasing n-3 fatty acids and reducing n-6 linoleic acid can decrease the duration and frequency of headaches, but did not significantly improve quality of life.
BMJ-BRITISH MEDICAL JOURNAL
(2021)
Review
Pharmacology & Pharmacy
Michael J. Iadarola, Dorothy Cimino Brown, Alexis Nahama, Matthew R. Sapio, Andrew J. Mannes
Summary: The challenge in analgesic drug development lies in determining the effectiveness of potential candidates in human populations. While cell and rodent models are important for initial screening and mechanism exploration, larger animal models like companion canines can enhance translational predictability for human analgesic performance.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Clinical Neurology
Michael J. Iadarola, Matthew R. Sapio, Andrew J. Mannes
Summary: A strong need for a new foundational molecular framework for human nervous system research at the nociceptive level exists. Comprehensive and quantitative capabilities for analyzing nociceptive tissues have been developed, but strong organization and infrastructures are required for multisite tissue recovery, molecular analyses, data sharing, and long-term archiving. Building human nociceptive tissue banks for multi-omic research requires sustained effort and careful characterization of pain problems from donors.
Article
Multidisciplinary Sciences
Bradford E. Hall, Emma Macdonald, Margaret Cassidy, Sijung Yun, Matthew R. Sapio, Pradipta Ray, Megan Doty, Pranavi Nara, Michael D. Burton, Stephanie Shiers, Abhik Ray-Chaudhury, Andrew J. Mannes, Theodore J. Price, Michael J. Iadarola, Ashok B. Kulkarni
Summary: This study investigated the intraganglionic pathology and mechanisms causing neuropathic pain in patients with Type 2 diabetes mellitus. The transcriptome analysis revealed increased levels of inflammation-associated transcripts and frequent downregulation of neuronally-related genes in patients with painful diabetic peripheral neuropathy.
SCIENTIFIC REPORTS
(2022)
Article
Neurosciences
Jenny J. Kim, Matthew R. Sapio, Fernando A. Vazquez, Dragan Maric, Amelia J. Loydpierson, Wenting Ma, Carlos A. Zarate, Michael J. Iadarola, Andrew J. Mannes
Summary: This study investigates the molecular pharmacological effects of ketamine in rats and finds that ketamine can stimulate neuronal transcription processes, leading to sustained modulation of regional excitation and plasticity in the brain. It also suggests that the administration of ketamine may result in an overshoot of transcriptional processes and an oscillation of plasticity during the transition to a new phase of neuronal regulation.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Neurosciences
Wenting Ma, Matthew R. Sapio, Allison P. Manalo, Dragan Maric, Mary Kate Dougherty, Taichi Goto, Andrew J. Mannes, Michael J. Iadarola
Summary: Primary afferent neurons in the dorsal root ganglia (DRG) play a crucial role in transmitting peripheral nociceptive signals and regulating analgesic control. The coexpression of Trpv1 and Oprm1 in these neurons may explain the remarkable efficacy of opioid drugs administered at the level of the DRG-spinal synapse.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Anesthesiology
Muhammad Yousef, Diana Bharucha-Goebel, Dimah Saade, Gilberto Averion, Carsten G. Bonnemann, Zenaide M. N. Quezado
Summary: A clinical trial involving 14 patients with Giant Axonal Neuropathy (GAN) undergoing anesthesia showed few nonsignificant perianesthetic complications, indicating the safety and tolerability of shorter procedural sedation and anesthesia for patients with this rare and potentially fatal disease.