Review
Physiology
Francisco J. Gonzalez-Gonzalez, Mary Rodriguez Ziccardi, Mark D. McCauley
Summary: SARS-CoV-2, identified in 2019, led to a massive pandemic with millions of cases and deaths globally. Infection has been recognized to affect multiple systems, including causing thromboembolic cerebrovascular events in the nervous system, often associated with poor prognosis. Understanding these mechanisms is crucial for effective prevention and treatment strategies to reduce the impact on patients' health and mortality.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Medicine, General & Internal
Aina Anton, Genis Camprecios, Valeria Perez-Campuzano, Lara Orts, Joan Carles Garcia-Pagan, Virginia Hernandez-Gea
Summary: This review summarizes the development mechanisms of portal vein thrombosis and discusses future directions in the study of this important complication of cirrhosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Hematology
N. A. Podoplelova, D. Y. Nechipurenko, A. A. Ignatova, A. N. Sveshnikova, M. A. Panteleev
Summary: Platelets play a significant role in accelerating blood coagulation, particularly a unique subset of procoagulant platelets with distinct characteristics and functions that are important in hemostasis and thrombosis.
Article
Medicine, Research & Experimental
Alec A. Schmaier, Papa F. Anderson, Siyu M. Chen, Emale El-Darzi, Ivan Aivasovsky, Milan P. Kaushik, Kelsey D. Sack, H. Criss Hartzell, Samir M. Parikh, Robert Flaumenhaft, Sol Schulman
Summary: The study found that endothelial cells can switch from an anticoagulant surface to a procoagulant surface under pathogenic stimuli, promoting thrombotic cardiovascular disease. Two TMEM16 family members, TMEM16F and TMEM16E, were identified as crucial for the procoagulant activity of endothelial cells. In addition, the TMEM16 inhibitor benzbromarone was found to prevent endothelial cell procoagulant activity and thrombus formation.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Hematology
Yuchuan Yuan, Jiaxing Liu, Hongli Duan, Chengyuan Zhang, Wenxing Wu, Qin Qin, Jie Lou, Qing Zhang, Qin Wang, Xiaohui Li, Xin Zhou
Summary: Researchers have developed an anticoagulant/procoagulant self-converting and bleeding site-targeting systemic nanohemostat that can quickly control noncompressible bleeding without the risk of thrombosis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Jens Van Bael, Aline Vandenbulcke, Abdelhakim Ahmed-Belkacem, Jean-Francois Guichou, Jean-Michel Pawlotsky, Jelle Samyn, Arjan D. Barendrecht, Coen Maas, Simon F. De Meyer, Karen Vanhoorelbeke, Claudia Tersteeg
Summary: Inhibiting CypD activity with novel non-immunosuppressive cyclophilin inhibitors (SMCypIs) can effectively reduce procoagulant platelet formation and provide a promising strategy for limiting thrombosis without affecting normal platelet function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Chih-Hsin Wang, Juin-Hong Cherng, Chuan-Chieh Liu, Tong-Jing Fang, Zhi-Jie Hong, Shu-Jen Chang, Gang-Yi Fan, Sheng-Der Hsu
Summary: Chitosan dressing demonstrated excellent antimicrobial and procoagulant properties, accelerating wound healing by protecting against microbial infection and promoting the growth of beneficial microbiota. The dressing exhibited good biocompatibility and outstanding blood absorption ability, inhibiting bacterial growth up to 8 days post-surgery. These findings suggest that chitosan dressing provides a suitable environment for beneficial microbiota and is effective in promoting wound repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Jihe Li, Dongxia Tong, Ying Wang, Yue Liu, Xiaohui Zhang, Na Liu, Shipeng Wang, Yang Xu, Yang Li, Xinhua Yin, Wenxiu Liu, Yibing Shao
Summary: This study revealed a previously unrecognized link between hypercoagulability and NETs in essential hypertension (EH) patients. The formation of NETs accelerated blood clotting, leading to thrombosis. Blockade of NETs may represent a new therapeutic target for preventing thrombosis in EH patients.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Hematology
Natasha Letunica, Suelyn Van den Helm, Conor McCafferty, Ella Swaney, Tengyi Cai, Chantal Attard, Vasiliki Karlaftis, Paul Monagle, Vera Ignjatovic
Summary: Proteomics is an innovative approach to study all proteins in a given cell, tissue or organism. Recent technological advancements have increased its application in thrombosis and hemostasis research, allowing for the identification of novel markers associated with disease diagnosis and prognosis.
THROMBOSIS AND HAEMOSTASIS
(2022)
Review
Cell Biology
Frederik Denorme, Robert A. Campbell
Summary: Platelets play a crucial role in maintaining hemostasis, but dysregulated activation can lead to thrombosis or bleeding. Aggregatory platelets and procoagulant platelets have distinct roles in thrombosis and hemostasis. Procoagulant platelets, a highly activated subpopulation, express specific proteins that facilitate the binding of coagulation factors and the generation of thrombin.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Oncology
Maaike Y. Kapteijn, Shanna Zwaan, Esther ter Linden, El Houari Laghmani, Rob F. P. van den Akker, Araci M. R. Rondon, Sabina Y. van der Zanden, Jacques Neefjes, Henri H. Versteeg, Jeroen T. Buijs
Summary: This study found a promoting role of the commonly used chemotherapeutic agents Temozolomide and Lomustine on tissue factor (TF) procoagulant activity and gene/protein expression in glioblastoma (GBM) cell lines. This may explain the increased risk of thrombosis in GBM patients undergoing chemotherapy. Therefore, GBM patients with high TF levels may benefit from anticoagulant treatment to decrease the risk of chemotherapy-induced thrombosis.
Article
Hematology
Sarah L. Millington-Burgess, Matthew T. Harper
Summary: The study revealed that R5421 can reduce platelet PS exposure and inhibit thrombin generation by maintaining flippase activity in procoagulant platelets, without directly inhibiting scramblase activity.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Engineering, Biomedical
Rodrigo Mendez Rojano, Mansur Zhussupbekov, James F. Antaki
Summary: This study investigates the risk of thrombosis around the VAD inlet cannula and reveals the crucial role of biochemical factors in thrombus formation, taking a step towards the long-term objective of incorporating clinically relevant pharmacological kinetics into simulations of inflow cannula thrombosis.
Article
Medical Laboratory Technology
Yaxin Chu, Han Guo, Yuncong Zhang, Rui Qiao
Summary: Platelets are crucial in hemostasis, with aggregating platelets attracting others to injury sites and procoagulant platelets promoting clot formation. Procoagulant platelets also play a proinflammatory role by releasing microparticles and polyphosphate, warranting further study for potential disease prevention strategies.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Nanoscience & Nanotechnology
Monique Etnea Machado, Priscila de Souza Furtado, Cristina da Costa Bernardes Araujo, Alice Simon, Marcela Cristina de Moraes, Luiz Claudio Rodrigues Pereira da Silva, Flavia Almada do Carmo, Lucio Mendes Cabral, Plinio Cunha Sathler
Summary: The study successfully developed RXB-loaded PLGA/poloxamer nanoparticles with favorable physicochemical characteristics, effective time-dependent anticoagulant activity, and non-toxic profile. These nanoparticles have the potential to be promising new therapies for the treatment of thrombotic disorders.
Article
Biology
Alan E. Mast, Alisa S. Wolberg, David Gailani, Michael R. Garvin, Christiane Alvarez, J. Izaak Miller, Piet Jones, Bruce Aronow, Daniel Jacobson
Summary: The previous study compared transcriptome data from hospitalized COVID-19 patients and control patients without COVID-19, showing changes in procoagulant and fibrinolytic gene expression in the lungs of COVID-19 patients. However, the findings were challenged due to sample issues. Nevertheless, upon revisiting the analyses, the researchers found that the new analyses were consistent with their original conclusions.
Article
Hematology
Alisa S. Wolberg, Yaqiu Sang
Summary: This article discusses the pathogenesis of venous thromboembolism and focuses on the role of fibrinogen and factor XIII in thrombus formation and resolution, providing insights for the development of new anticoagulant strategies.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biophysics
Kenji Miyazawa, Aaron L. Fogelson, Karin Leiderman
Summary: Blood coagulation is regulated by platelet surfaces, clotting factors, and inhibitors such as antithrombin (AT). Heparin enhances the inhibitory effect of AT on coagulation. Our study found that AT alone is effective at low tissue factor densities, but in the presence of heparin, it can greatly alter or shut down thrombin generation. Additionally, inactivating a single enzyme cannot substantially suppress thrombin generation.
BIOPHYSICAL JOURNAL
(2023)
Article
Hematology
Anton Ilich, Terry B. Gernsheimer, Darrell J. Triulzi, Heather Herren, Siobhan P. Brown, Lori A. Holle, Andrew T. Lucas, Bas de Laat, Nahed El Kassar, Alisa S. Wolberg, Susanne May, Nigel S. Key
Summary: This study found that using TXA did not show superiority over placebo in preventing high-grade bleeding in patients with severe thrombocytopenia. The study also did not find an association between fibrinolysis assays and bleeding risk.
Article
Biophysics
Kenji Miyazawa, Aaron L. Fogelson, Karin Leiderman
Summary: Blood coagulation is a self-repair process regulated by activated platelet surfaces, clotting factors, and inhibitors. Recently, a new role for TFPI in inhibiting thrombin generation on activated platelet surfaces has been discovered, which is independent of TF:VIIa. This study found that this TF-independent inhibitory mechanism is more effective than the TF-dependent one.
BIOPHYSICAL JOURNAL
(2023)
Article
Hematology
Steven P. Grover, Tomohiro Kawano, Jun Wan, Pansakorn Tanratana, Zsofia Polai, Young J. Shim, Omri Snir, Sigrid Braekkan, Sophia Dhrolia, Rohan R. Kasthuri, Pavan K. Bendapudi, Keith R. McCrae, Alisa S. Wolberg, John-Bjarne Hansen, Henriette Farkas, Nigel Mackman
Summary: C1INH is a serine protease inhibitor that regulates multiple biological pathways. Deficiency of C1INH is associated with hereditary angioedema (HAE) and increased risk of coagulation activation and thrombosis.
Article
Hematology
Jun Wan, Pansakorn Tanratana, Mark Roest, Andras Gruber, Rafal Pawlinski, Alisa S. Wolberg, Nigel Mackman, Steven P. Grover
Summary: This study describes a novel mouse whole blood TG assay to assess the amplifying roles of intrinsic pathway factors in mouse coagulation. The assay has enhanced sensitivity to FXI and FIX-dependent amplification of coagulation, suggesting an important role of blood cells in this process.
Article
Multidisciplinary Sciences
Emma Bouck, Marios Arvanitis, William K. Osburn, Yaqiu L. Sang, Paula J. Reventun, Homa S. Ahmadzia, Nicholas P. Smith, Charles P. Lowenstein, Alisa P. Wolberg
Summary: Oral contraceptive (OC) use increases the risk of venous thromboembolism. This study investigates the effect of OC hormones on endothelial cell (EC) procoagulant activity and the potential interaction between OC hormones and estrogen receptors and inflammation. The results show that the OC hormones ethinyl estradiol and drospirenone do not directly enhance the thrombin generation potential of primary ECs in vitro.
Editorial Material
Hematology
Eric Boilard, Kellie R. Machlus
Article
Hematology
Julie Hahn, Jan Bressler, Arce Domingo-Relloso, Ming-Huei Chen, Daniel L. McCartney, Alexander Teumer, Jenny van Dongen, Marcus E. Kleber, Dylan Aissi, Brenton R. Swenson, Jie Yao, Wei Zhao, Jian Huang, Yujing Xia, Michael R. Brown, Ricardo Costeira, Eco J. C. de Geus, Graciela E. Delgado, Dre'Von A. Dobson, Paul Elliott, Hans J. Grabe, Xiuqing Guo, Sarah E. Harris, Jennifer E. Huffman, Sharon L. R. Kardia, Yongmei Liu, Stefan Lorkowski, Riccardo E. Marioni, Matthias Nauck, Scott M. Ratliff, Maria Sabater-Lleal, Tim D. Spector, Pierre Suchon, Kent D. Taylor, Florian Thibord, David-Alexandre Tregouet, Kerri L. Wiggins, Gonneke Willemsen, Jordana T. Bell, Dorret I. Boomsma, Shelley A. Cole, Simon R. Cox, Abbas Dehghan, Andreas Greinacher, Karin Haack, Winfried Marz, Pierre-Emmanuel Morange, Jerome I. Rotter, Nona Sotoodehnia, Maria Tellez-Plaza, Ana Navas-Acien, Jennifer A. Smith, Andrew D. Johnson, Myriam Fornage, Nicholas L. Smith, Alisa S. Wolberg, Alanna C. Morrison, Paul S. de Vries
Summary: This study examined the association between DNA methylation and circulating fibrinogen levels, and identified 83 CpG sites that are associated with fibrinogen levels. These associations are partially driven by inflammatory pathways shared by both fibrinogen and CRP.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Dre'Von A. Dobson, Lori A. Holle, Feng-Chang Lin, Jennifer E. Huffman, James P. Luyendyk, Matthew J. Flick, Nicholas L. Smith, Paul S. de Vries, Alanna C. Morrison, Alisa S. Wolberg
Summary: This study aimed to understand the (patho) physiological actions of fibrinogen by characterizing the genetic contribution to its synthesis. By inducing HepG2 cells to express fibrinogen and observing the effects of small interfering RNA knockdown of different candidate genes, several genes interacting with fibrinogen production were identified. This approach can reveal cellular mechanisms of fibrinogen and fibrin(ogen)-mediated (patho) physiological mechanisms.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Stephanie E. Reitsma, Lori A. Holle, Emma G. Bouck, Dougald M. Monroe, Alan E. Mast, John Burthem, Paula H. B. Bolton-Maggs, Gillian N. Gidley, Alisa S. Wolberg
Summary: This study suggests that tissue factor pathway inhibitor (TFPI) may play a role in modifying the bleeding risk in individuals with factor XI (FXI) deficiency. In vitro experiments showed that inhibition of TFPI enhanced clot formation and reduced fibrinolysis in FXI-deficient plasma, while increased TFPI had the opposite effects. Higher TFPI concentrations were observed in FXI-deficient individuals with bleeding compared to controls, and total plasma TFPI correlated with clotting and fibrinolysis parameters associated with bleeding risk. These findings indicate that TFPI may be a potential modifier of bleeding risk in FXI-deficient individuals.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Immunology
Edward M. Conway, Nigel Mackman, Ronald Q. Warren, Alisa S. Wolberg, Laurent O. Mosnier, Robert A. Campbell, Lisa E. Gralinski, Matthew T. Rondina, Frank L. van de Veerdonk, Karin M. Hoffmeister, John H. Griffin, Diane Nugent, Kyung Moon, James H. Morrissey
Summary: In this article, the authors discuss the emerging understanding of COVID-19-associated coagulopathy and the need to comprehend its cellular and molecular mechanisms. They categorize the current understanding into three main pathological mechanisms and address key questions and research gaps in order to improve diagnosis and treatment. The suitability of animal models for studying this condition is also considered.
NATURE REVIEWS IMMUNOLOGY
(2022)
Review
Hematology
Sarah Nersesian, Michelle Sholzberg, Mary Cushman, Alisa S. Wolberg
Summary: Illustrated review articles consist of capsules or panels of visuals that provide an up-to-date overview of a topic. They aim to be more accessible than traditional written reviews, facilitating effective knowledge translation and dissemination. This article summarizes the process of developing an illustrated review, emphasizes the importance of storytelling, and provides tools and resources for creating effective illustrated review articles.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Woosuk S. Hur, David S. Paul, Emma G. Bouck, Oscar A. Negron, Jean-Marie Mwiza, Lauren G. Poole, Holly M. Cline-Fedewa, Emily G. Clark, Lih Jiin Juang, Jerry Leung, Christian J. Kastrup, Tatiana P. Ugarova, Alisa S. Wolberg, James P. Luyendyk, Wolfgang Bergmeier, Matthew J. Flick
Summary: Mutations in the alpha C-region of the fibrinogen A alpha chain can cause hypodysfibrinogenemia, resulting in a significant reduction in fibrinogen levels. However, these mutations do not compromise hemostatic and antimicrobial functions, and even provide protection against thrombosis.