Article
Virology
Mara Elena Heinrichs, Daniele De Corte, Bert Engelen, Donald Pan
Summary: Flow cytometry has been used to understand the impact of viruses on shaping marine microbial communities and biogeochemical cycles in the pelagic zone. A new protocol has been developed for the enumeration of marine sediment viruses by flow cytometry, enabling fast and accurate quantification of viral abundances and opening up options for virus sorting, targeted viromics, and single-virus sequencing. Further optimization may be needed for sediments with low biomass or high organic content.
Article
Andrology
Yanan Zhang, Juan Cheng, Jianping Ou, Zhuojie Liu, Yonghui Liu, Zhi-Jun Zang
Summary: This study found that after using the DDGC method to process semen in male infertility testing, the diagnostic accuracy of sperm concentration, total sperm number, and sperm morphology was significantly improved, while there were no significant changes in other parameters after treatment. Therefore, DDGC may be an effective method to improve the diagnostic accuracy of male infertility.
Article
Genetics & Heredity
U. Punjabi, H. Van Mulders, L. Van de Velde, I Goovaerts, K. Peeters, W. Cassauwers, T. Lyubetska, K. Clasen, P. Janssens, O. Zemtsova, E. Roelant, D. De Neubourg
Summary: Sperm parameters did not significantly affect pregnancy outcomes in IUI cycles, with only female and male ages showing a significant association. Short time intervals between semen production, analysis, and IUI significantly influenced clinical pregnancies and live births after adjusting for age.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Nanoscience & Nanotechnology
Steven A. Vasilescu, Lin Ding, Farin Yazdan Parast, Reza Nosrati, Majid Ebrahimi Warkiani
Summary: This article presents a 3D printed microfluidic sperm selection device (MSSP) that mimics the behavior of sperm selection and has higher sperm motility and lower DNA fragmentation and apoptosis. It demonstrates that MSSP is easy to use and mimics the biological function of the female reproductive tract during conception.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Multidisciplinary Sciences
Li-fang Guo, Liu Wang, Sai Ren, Ning Su, Kun Wei, Xian-Ge Sun, Xiao-Dong Ren, Qing Huang
Summary: A novel 3D printing leukocyte sorting accumulator combined with centrifugation was demonstrated to achieve label-free initial leukocyte enrichment based on cell density and size. The upgraded design, leukocyte sorting accumulator-3, optimized the recovery of buffy coats compared to earlier models, with mean leukocyte recovery rates of 96.2 +/- 2.38% by pushing method and 94.4 +/- 0.8% by pulling method. New procedures for preliminary leukocyte enrichment are essential for modern clinical and basic research assays.
Article
Agriculture, Dairy & Animal Science
Maria V. O. Santos, Andreia M. Silva, Leonardo V. C. Aquino, Lhara R. M. Oliveira, Samara S. J. Moreira, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira
Summary: This study compared three commonly used methods, swim-up, Percoll gradient, and washing by centrifugation, for extracting sperm from collared peccaries. The results showed that swim-up method had poor efficiency in isolating motile sperm, while Percoll gradient and washing by centrifugation had similar quality and fertilization capacity of the sperm. Therefore, Percoll gradient and washing by centrifugation are recommended for sperm extraction in collared peccaries.
Article
Agriculture, Dairy & Animal Science
Nicole Sugai, Stephen Werre, Julie Cecere, Orsolya Balogh
Summary: The study aimed to determine the clinically relevant centrifugation parameters for processing canine semen. It was hypothesized that higher gravitational force and longer centrifugation time would improve spermatozoa recovery rate but decrease semen quality. The results showed that centrifugation within the range of 400 g-900 g for 5-10 min is appropriate for processing canine semen.
Review
Immunology
Madalina Preda, Loredana Sabina Cornelia Manolescu, Razvan Daniel Chivu
Summary: Alpha herpes simplex viruses pose a significant public health problem, affecting people of all ages. From common cold sores to severe conditions like encephalitis and newborn mortality, the virus produces varying levels of pathology. While there is an efficient vaccine for varicella-zoster virus, there is no available vaccine for herpes simplex virus 1 and 2 despite several approaches, including trivalent subunit vaccines and bioinformatic studies. However, recent promising attempts, such as a trivalent vaccine and a multivalent DNA vaccine, offer hope for future solutions.
Article
Multidisciplinary Sciences
KangSheng Liu, XiaoDong Mao, Feng Pan, YaJun Chen, Ruifang An
Summary: Routine semen analysis is limited in assessing male infertility caused by sperm DNA defects. This study analyzed the association of sperm DNA fragmentation index (DFI) with semen routine and IVF-ET/ICSI outcomes. Sperm DFI was negatively associated with sperm concentration and pregnancy rate. Antioxidants can reduce DNA fragmentation. Further investigation is needed to determine the significance of DFI as a predictor of pregnancy outcomes in ART.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Catielen Paula Pavi, Isabella Dai Pra, Rafael Dorighello Cadamuro, Isamu Kanzaki, Jhuly Wellen Ferreira Lacerda, Louis Pergaud Sandjo, Roberto Messias Bezerra, Jorge Federico Orellana Segovia, Gislaine Fongaro, Izabella Thais Silva
Summary: This study evaluated the activity of seventeen extracts derived from eight Amazonian medicinal plants against herpes simplex virus and chikungunya virus. Four extracts exhibited significant activity against both viruses, including the drug-resistant strains. Virucidal tests showed a higher level of virucidal activity compared to antiviral activity.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Lauren A. Sadowski, Gregory M. Lesko, Chad Suissa, Rista Upadhyay, Prashant J. Desai, Barry J. Margulies
Summary: Plaquing viruses is a challenging method that requires meticulous attention to detail, especially for new students. This manuscript provides a detailed description of handling host cells, using a viscous medium, and a staining procedure to produce reproducible results, with an accompanying video to demonstrate finer distinctions frequently missed in instruction.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Review
Medicine, General & Internal
Anna Duckworth, Hilary J. Longhurst, Jane K. Paxton, Chris J. Scotton
Summary: Pulmonary fibrosis is a serious lung disease that can result from genetic or environmental exposures, or may be idiopathic. Recent evidence suggests short telomeres may play a causal role in PF. The involvement of herpes viruses in PF disease remains a debated topic with inconclusive evidence.
FRONTIERS IN MEDICINE
(2021)
Article
Public, Environmental & Occupational Health
Zhiheng Chen, Lingxiao Jiang, Hui Wang, Wumin Quan, Li Yang, Chunquan Ou, Ling Sun
Summary: This study evaluated the efficiency of different sperm preparation procedures in bacterial clearance. The results show that the combined density-gradient centrifugation and swim-up method is more effective in eliminating bacteria from semen samples compared to either method alone.
JOURNAL OF MENS HEALTH
(2022)
Review
Pharmacology & Pharmacy
Hanna H. Schalkwijk, Robert Snoeck, Graciela Andrei
Summary: Herpes simplex viruses (HSV) can cause significant morbidity and mortality in both immunocompetent and immunocompromised individuals, and the nucleoside analogue acyclovir has greatly improved the management of HSV infections. However, acyclovir resistant strains have been reported, especially in immunocompromised patients. This review discusses the prevalence of acyclovir resistance in HSV infections and alternative antiviral treatments.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Engineering, Chemical
Yuichi Kato, Takahiro Morimoto, Kazufumi Kobashi, Tetsuji Yamaguchi, Tetsuya Mori, Takushi Sugino, Toshiya Okazaki
Summary: In this study, we investigated the sedimentation of porous nylon-6 particles with and without a density gradient. The analysis of inner fluid buoyancy was used to estimate the size and particle porosity in the liquid phase.