Article
Infectious Diseases
Xiaodong Wang, Paride Abliz, Shuwen Deng
Summary: This study investigated macrolide resistance in Treponema pallidum from patients with latent syphilis in Xinjiang, China. The results indicated the presence of macrolide resistance in T. pallidum in Xinjiang, with A2058G being the predominant resistance mechanism. Blood samples may serve as suitable specimens for detecting resistant mutations in T. pallidum in patients with latent syphilis, even without clinical symptoms.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
J. G. E. Laumen, S. S. Manoharan-Basil, E. Verhoeven, S. Abdellati, I De Baetselier, T. Crucitti, B. B. Xavier, S. Chapelle, C. Lammens, C. Van Dijck, S. Malhotra-Kumar, C. Kenyon
Summary: This study characterized the genetic pathways leading to high-level azithromycin resistance in Neisseria gonorrhoeae by using a customized morbidostat to subject reference strains to sustained drug pressure. The results showed stepwise evolution of resistance, with key mutations identified in ribosomal proteins and efflux pumps. High-level resistance was consistently associated with mutations in the 23S ribosomal RNA, including well-known A2059G and C2611T mutations.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Ivica Samanic, Blanka Dadic, Zeljka Sanader Marsic, Mia Dzelalija, Ana Maravic, Hrvoje Kalinic, Pavle Vrebalov Cindro, Zeljko Sundov, Marija Tonkic, Ante Tonkic, Jonatan Vukovic
Summary: This study investigated the molecular genetic mechanisms of clarithromycin and levofloxacin resistance in Helicobacter pylori infections in patients from Croatia. Point mutations in the 23S rRNA, gyrA, and gyrB genes were found to alter target sites or protein structure, leading to reduced efficacy of standard antibiotics. Molecular docking analysis revealed that resistance-related mutations resulted in decreased susceptibility to clarithromycin and levofloxacin due to significant differences in non-covalent interactions, ultimately resulting in antibiotic resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Zhili Guo, Liyong Liu, Jie Gong, Na Han, Lihua He, Weijing Wang, Fanliang Meng, Xiuliang Xia, Jianzhong Zhang, Fei Zhao
Summary: This study analysed the molecular features and antimicrobial susceptibility of Mycoplasma pneumoniae isolates from paediatric patients in Weihai, China, in 2019. The results showed that there were two genotypes present in a relatively equivalent ratio, with genotype 2 slightly predominant, and the overall antimicrobial resistance rate was close to 100%.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Immunology
Rasmus Desdorf, Niles Moller Andersen, Ming Chen
Summary: This study revealed a high prevalence of Mycoplasma genitalium and macrolide resistance-associated mutations in Southern Jutland, Denmark, indicating the importance of including M. genitalium testing in routine sexually transmitted infection screening.
Review
Infectious Diseases
Qian Zhou, Jingwei Liu, Shaochun Chen, Wenqi Xu, Yan Han, Yueping Yin
Summary: The meta-analysis shows that the C2611T mutation in the 23S rRNA of N. gonorrhoeae is valuable for molecular diagnostics of moderate-level azithromycin resistance, particularly when the moderate level is set at 4-128 mg/L. This rapid molecular detection method can be used for rapid identification of drug-resistant isolates in clinical settings.
Article
Immunology
Wenyin He, Ying Yuan, Junyu Liang, Xuejiao Fan, Lei Li, Xingfei Pan
Summary: This study developed a nested real-time PCR method for simultaneous detection of 23S rRNA and parC genotypes in Mycoplasma genitalium, which can be used to determine macrolide and fluoroquinolone resistance. The method showed high sensitivity, rapidity, and ease of interpretation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Xiao Han, Guiling Zou, Jiaren Liu, Chun Yang, Xuefei Du, Guoyu Chen, Zhe Sun, Xinyu Zhang, Yu Sun, Wanying Zhang, Xiaofeng Jiang
Summary: This study investigated the molecular characteristics and potential resistance mechanisms of linezolid-resistant Staphylococcus capitis isolates from a tertiary hospital in China. A potentially novel mutation, C2128T, that may have an impact on bacterial resistance was identified.
Article
Infectious Diseases
Naoki Kawakami, Ho Namkoong, Fumitake Saito, Masami Ishizaki, Masahiko Yamazaki, Keiko Mitamura
Summary: This study aimed to investigate the prevalence and clinical characteristics of macrolide-resistant M. pneumoniae infection in different age groups. The results showed no significant differences in the prevalence of macrolide-resistant M. pneumoniae infection among different age groups, and previous macrolide treatment contributes to drug resistance.
JOURNAL OF INFECTION AND CHEMOTHERAPY
(2021)
Article
Immunology
Hongliang Chen, Li Wang, Lanhua Zhao, Lipei Luo, Shuling Min, Yating Wen, Wenbo Lei, Mingyi Shu, Zhongyu Li
Summary: This study revealed differences in vaginal microbiota between women with tubal infertility who were infected with C. trachomatis and healthy controls. Women infected with C. trachomatis exhibited a unique Lactobacillus iners-dominated vaginal microbiota, rather than Lactobacillus crispatus. After standard treatment, the vaginal microbiota could potentially be restored.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Helene Gingras, Flora Peillard-Fiorente, Chantal Godin, Kevin Patron, Philippe Leprohon, Marc Ouellette
Summary: Two strains of Streptococcus pneumoniae with or without erm(B) were selected for solithromycin resistance in vitro. Mutants with decreased solithromycin susceptibility were obtained and characterized by next-generation sequencing. Various mutations in ribosomal proteins, 23S rRNA, phosphate transporter subunits, helicase CshB, and erm(B)L leader peptide were identified.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Elizaveta Dmitrievna Shedko, Guzel Anvarovna Khayrullina, Elena Nikolaevna Goloveshkina, Vasiliy Gennadevich Akimkin
Summary: The study found that the prevalence of macrolide- and fluoroquinolone-resistant Mycoplasma genitalium in Moscow has been increasing annually, with all three kits showing effectiveness in monitoring the prevalence of mutations.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Shumaiza Anis, Shakeel R. Farooqi, Saad K. Niaz
Summary: The study aimed to characterize clarithromycin resistance and associated mutations in clinical isolates of H. pylori in Pakistan. Results showed extensive diversity of domain V mutations in H. pylori, with multiple mutations potentially conferring endurance against clarithromycin. Further investigation into the molecular mechanisms of antibiotic resistance in H. pylori is deemed crucial at this stage.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Judit Alarcon-Millan, Jose Bonilla-Delgado, Gloria Fernandez-Tilapa, Nayeli Goreti Nieto-Velazquez, Monica Sierra-Martinez, Victor Manuel Alvarado-Castro, Enoc Mariano Cortes-Malagon
Summary: This study investigated the prevalence of clarithromycin resistance-associated mutations in Mexican patients infected with Helicobacter pylori. The results showed a high prevalence of H. pylori infection (84.3%) among patients with gastric diseases, with the vacA s1m1/cagA+ genotype being the most frequent (44.8%). The prevalence of clarithromycin resistance-associated mutations was 19.8%, with the A2143G and A2142C mutations being the most common. Furthermore, a significant association was found between the A2142G mutation and the vacA s1m1/cagA+ genotype.
Article
Infectious Diseases
Lucia Malisova, Vladislav Jakubu, Katarina Pomorska, Martin Musilek, Helena Zemlickova
Summary: The main mechanisms of linezolid resistance in linezolid-resistant enterococci in the Czech Republic were found to be the G2576T mutation in the domain V of 23S rRNA and the presence of the optrA gene, with some isolates also showing resistance to tigecycline and vancomycin.