期刊
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
卷 293, 期 6, 页码 1089-1098出版社
WILEY
DOI: 10.1002/ar.21141
关键词
Hypobaric hypoxia; lung tissue; nitric oxide; endothelial nitric oxide synthase; inducible nitric oxide synthase; reoxygenation
资金
- Ministry of Education and Sciences of Spain [SAF-2003-04398-C02-02]
Nitric oxide plays a critical role in many physiological and physiopathological processes in the lung Changes in the NO/NOS (Nitric Oxide/ Nitric Oxide Synthase) system after hypoxia situations remain controversial in this organ, so that the aim of this work is to perform a complete study of this system in the hypoxic lung after different reoxygenation times ranging from 0 h to 5 days posthypoxia. This is a novel follow-up study carried out in Wistar rats submitted for 30 min to acute hypobaric hypoxia. We measured endothelial and inducible NOS (eNOS, iNOS) mRNA and protein expression, location, and in situ NOS activity as well as nitrated protein expression and location In addition, NO levels were indirectly quantified (NOx) as well as the apoptosis level Results showed an increase in eNOS mRNA, protein, activity as well as eNOS positive immunostaining at 0 h posthypoxia, coinciding with raised NOx levels Contrary, iNOS, nitrated protein expression and apoptosis level augmented during the final reoxygenation times The lung NO/NOS system provokes two responses to the hypoxia/reoxygenation processes. (i) eNOS is responsible of the immediate response, producing NO, which causes vasodilation and bronchodilation, and (ii) iNOS is related to the second late response, which seems to be involved in some of the deleterious consequences that hypoxia induces in the lung Anat Rec, 293 1089-1098, 2010 (C) 2010 Wiley-Liss, Inc
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