期刊
ANALYTICAL SCIENCES
卷 35, 期 1, 页码 39-43出版社
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.18SDP01
关键词
Electrochemical bioimaging; LSI-based amperometric device; multiple detection; printed polycaprolactone; cell culture in hydrogels; embryonic stem cell
资金
- Japan Society for the Promotion of Science (JSPS) [16H02280, 15H03542, 18H01840, 18H01999, 16K14012, 15H05415]
- Asahi Glass Foundation
- Program for Creation of Interdisciplinary Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University
- Grants-in-Aid for Scientific Research [16K14012] Funding Source: KAKEN
Tissue engineering requires analytical methods to monitor cell activity in hydrogels. Here, we present a method for the electrochemical imaging of cell activity in hydrogels embedded in printed polycaprolactone (PCL) scaffolds. Because a structure made of only hydrogel is fragile, PCL frameworks are used as a support material. A grid-shaped PCL was fabricated using an excluder printer. Photocured hydrogels containing cells were set at each grid hole, and cell activity was monitored using a large-scale integration-based amperometric device. The electrochemical device contains 400 microelectrodes for biomoleculc detection, such as dissolved oxygen and enzymatic products. As proof of the concept, alkaline phosphatase and respiration activities of embryonic stem cells in the hydrogels were electrochemically monitored. The results indicate that the electrochemical imaging is useful for evaluating cells in printed scaffolds.
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