期刊
ANALYTICAL SCIENCES
卷 26, 期 12, 页码 1261-1264出版社
SPRINGERNATURE
DOI: 10.2116/analsci.26.1261
关键词
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资金
- National High-tech R&D Program (863 Program) [2007AA09Z113]
- National Natural Science Foundation of China [20977055]
- Shandong Provincial Natural Science Foundation of China [Y2007E03]
- State Key Laboratory Foundation of Geological Processes and Mineral Resources [GPMR200924]
- Key Lab Foundation of Biogeology and Environmental Geology of Ministry of Education [BGEGF200804]
A simple and homogeneous method based on aptamer and pyrene moieties for the detection of K+ was developed. The aptamer was labeled by pyrene moiety at 3' end as the molecular recognition element. In the presence of K+, the complementary oligonucleotide labeled by pyrene moiety at 5' end was displaced from the aptamer, which was accompanied by a dramatic decrease of the excimer fluorescence of pyrene. However, the excimer fluorescence remains in the absence of the target. With optimum conditions, relative changes of the pyrene excimer fluorescence intensity were proportional to the concentrations of K+ in the range of 6.3 x 10(-4) to 1.0 x 10(-2) M with a detection limit of 5.0 x 10(-4) M. Importantly, in the presence of Na+, NH4+, Mg2+ and Ca2+ cations of biological fluids, this method was able to detect K+ with high selectivity. In a word, the assay seems to have great potential applications, especially in biological fluids due to its simplicity, specificity and homogeneous detection.
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