期刊
ANALYTICAL METHODS
卷 6, 期 16, 页码 6536-6540出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ay00843j
关键词
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资金
- National Natural Science Foundation of China [21273174]
- Municipal Science Foundation of Chongqing City [CSTC-2013jjB00002]
A sensitive and selective biosensor has been developed to detect the hydroxyl radical ((OH)-O-center dot), which was generated through the Fenton reaction. (OH)-O-center dot could oxidatively damage DNA and cleave it into pieces. Based on this, taking [Ru(NH3)(6)](3+) as the electrochemical indicator, (OH)-O-center dot was determined on a gold electrode with self-assembled DNA. The resulting electrochemical biosensor showed a wide linear range of detection from 125 pM to 625 nM with a detection limit of 80 pM and satisfactory selectivity.
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