Article
Microbiology
Ruining Li, Zhiwei Li, Chenxi Huang, Yifeng Ding, Jia Wang, Xiaohong Wang
Summary: This study reported the potential of a Staphylococcus phage named LSA2302 for the rapid detection of S. aureus. The phage's biological characteristics were identified, and genomic analysis revealed its lack of pathogenicity or drug resistance genes. A biosensing method for S. aureus detection was established using phage-functionalized magnetic beads (pMB) integrated with ATP bioluminescence assays.
Article
Chemistry, Analytical
Fangbin Xiao, Ju Liu, Xiaoyan Feng, Guoyang Xie, Wanqing Zhang, Hengyi Xu
Summary: This study developed a method based on magnetic nanoparticles and recombinase aided amplification for rapid capture and detection of Staphylococcus aureus in food samples. The method has low cost, short detection time, and high sensitivity, and can simultaneously analyze multiple bacteria, which is important for evaluating the microbiological safety of food samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Microbiology
Runan Cheng, Lei Li, Sihui Zhen, Honglei Liu, Zhouhui Wu, Yu Wang, Zhen Wang
Summary: In this study, a novel method based on immunomagnetic separation using immunomagnetic beads (IMBs) and recombinase polymerase amplification (RPA) was developed for the rapid detection of Staphylococcus aureus (S. aureus) in milk and pork. The IMBs-RPA method showed potential for food safety supervision and rapid early diagnosis of diseases due to its short detection time, high sensitivity, and specificity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agriculture, Dairy & Animal Science
Jiai Yan, Ruijin Yang, Suhuai Yu, Wei Zhao
Summary: Staphylococcus aureus is a prevalent foodborne pathogen that poses a threat to human health, especially with the emergence of multidrug-resistant strains like MRSA. The study found that the endolysin LysGH15 and its catalytic domain CHAP(LysGH15) exhibited strong antimicrobial efficacy against S. aureus, with differing optimal activity conditions. The sensitivity to NaCl concentration also varied between LysGH15 and CHAP(LysGH15), affecting their lytic activity levels.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Ryota Miyazawa, So Shimoda, Keiichi Matsuda, Ryuta Tobe, Tasuke Ando, Hiroshi Yoneyama
Summary: Staphylococcus aureus is a major pathogen in humans and livestock, causing serious issues such as bovine mastitis. This study monitored cases of intramammary infection caused by S. aureus in the Miyagi Prefecture, Japan, and analyzed the strains obtained from dairy farms. The study found recurrent cases of infection and identified a Methicillin-Sensitive SA (MSSA) ST398 strain isolated from mastitis-containing cows, which is the first confirmed case in Japan. The results highlight the importance of nationwide surveillance to manage the distribution of ST398 strains in dairy farms for human and animal health.
Article
Multidisciplinary Sciences
Aisling M. Towell, Cecile Feuillie, Pauline Vitry, Thaina M. Costa, Marion Mathelie-Guinlet, Sanja Kezic, Orla M. Fleury, Maeve A. McAleer, Yves F. Dufrene, Alan D. Irvine, Joan A. Geoghegan
Summary: Staphylococcus aureus interacts with the host protein corneodesmosin on atopic dermatitis corneocytes, taking advantage of the aberrant display of corneodesmosin caused by low natural moisturizing factor levels. The bacterial proteins FnBPB and ClfB bind to the N-terminal region of corneodesmosin, facilitating strong adherence of S. aureus to atopic dermatitis corneocytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Food Science & Technology
Guoyang Xie, Donggen Zhou, Guojing Zhao, Xiaoyan Feng, Zoraida P. Aguilar, Hengyi Xu
Summary: The study proposed a method for rapid and real-time detection of viable S. aureus in milk, combining PMAxx dye and RAA technology. It can accurately detect S. aureus in a short time, which is of great significance for effective monitoring of food safety.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Zhenzhen Zhang, Yanmin Song, Liyan Ma, Kunlun Huang, Zhihong Liang
Summary: This study investigated the presence of Staphylococcus aureus and Ochratoxin A (OTA) in pasteurized milk in ten urban Beijing districts, and found the co-occurrence of these two biohazards. The results indicate that when the cold chain is not guaranteed, the presence of S. aureus carrying enterotoxin genes in milk poses a risk to food safety, while the high detection rates and levels of OTA suggest it is a hidden risk.
Article
Microbiology
Xavier Vila-Farres, Karen Sauve, Jun Oh, Steven Swift, Boudewijn DeJonge, Jane E. Ambler, Raymond Schuch
Summary: Lysins are new protein-based antimicrobial candidates used to combat antibiotic resistance. The study evaluated the bacteriolytic activity of the antistaphylococcal lysin Exebacase, showing concentration-dependent killing against Staphylococcus aureus strains. Exebacase demonstrated rapid bactericidal activity within 3 h and dose-dependent lysis at low concentrations. Microscopy confirmed membrane dissolution and cell lysis. The novel modality of Exebacase provides a rapid and effective antimicrobial solution against S. aureus.
MICROBIOLOGY SPECTRUM
(2023)
Review
Biotechnology & Applied Microbiology
Wei Chen, Qingteng Lai, Yanke Zhang, Zhengchun Liu
Summary: The infection of Staphylococcus aureus and the spread of drug-resistant bacteria pose a serious global public health threat. Developing a fast, simple, low-cost, specific and sensitive detection method for S. aureus is of great significance. Aptasensors, as alternatives to traditional detection methods, have shown promising applications in S. aureus detection.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Spectroscopy
Yuwan Du, Hui Huang, Yuan Peng, Jiang Wang, Zhixian Gao
Summary: This study successfully predicted the concentration of Staphylococcus aureus enterotoxin B in prepared milk samples using Raman spectroscopy and chemometric methods.
JOURNAL OF RAMAN SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Jiandong Liu, Bin Liu, Juan Liu, Xing-Dao He, Jinhui Yuan, Zabih Ghassemlooy, Hamdi Torun, Yong-Qing Fu, Xuewu Dai, Wai Pang Ng, Richard Binns, Qiang Wu
Summary: A high quality factor (Q-factor) optical fiber interferometer based biosensing platform, combining single mode-core-only-single mode fiber (SCS) and multimode interference effect, is developed for ultra-high sensitivity biosensing. This biosensor is capable of effectively detecting concentrations of Staphylococcus aureus from 10 to 105 CFU/mL, with good sensitivity, stability, reproducibility and specificity, showing great potentials in diagnostics.
Article
Chemistry, Analytical
Jinghui Li, Yipeng Tang, Yunpeng Bai, Zhejun Zhang, Shaopeng Zhang, Tongyun Chen, Feng Zhao, Zhigang Guo
Summary: In this study, nanozymes with high catalytic performance were synthesized for rapid detection of Staphylococcus aureus. The proposed biosensor showed high sensitivity and specificity, making it suitable for clinical applications.
Article
Food Science & Technology
Xiaonan Zhao, Xiaomeng Yuan, Ming Hu, Yin Zhang, Lulu Li, Qing Zhang, Xuexia Yuan, Wenbo Wang, Yuqing Liu
Summary: The study revealed a relatively high prevalence of S. aureus in bulk tank milk from Shandong dairy farms, with the isolates carrying multiple virulence genes and exhibiting multiple antimicrobial resistance. The lurking threat of MRSA in raw milk emphasizes the need for surveillance programs starting at the farm level.
Article
Chemistry, Multidisciplinary
Pavel Acai, Martina Konuchova, L'ubomir Valik
Summary: The growth of two pairs of co-cultures with a starter culture of lactic acid bacteria was studied in milk at different temperatures, and it was found that the lactic acid bacteria not only induced an early stationary phase of Escherichia coli and Staphylococcus aureus but also affected their death phase. In co-cultures with lactic acid bacteria and Geotrichum candidum, the numbers of E. coli and S. aureus increased initially and then decreased significantly. This study provides knowledge support for the hygiene guidelines of short-ripened raw milk cheeses.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Analytical
K. Kivirand, A. Floren, M. Kagan, T. Avarmaa, T. Rinken, R. Jaaniso
Article
Chemistry, Analytical
Margarita Kagan, Gunnar Printsmann, Kairi Kivirand, Toonika Rinken
ANALYTICAL LETTERS
(2017)
Article
Biotechnology & Applied Microbiology
K. Kivirand, H. Somerik, M-L. Oldekop, R. Rebane, T. Rinken
ENZYME AND MICROBIAL TECHNOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
K. Mihklepp, K. Kivirand, M. Nikopensius, D. Peedel, M. Utt, T. Rinken
ENZYME AND MICROBIAL TECHNOLOGY
(2017)
Article
Chemistry, Analytical
Delia Juronen, Ave Kuusk, Kairi Kivirand, Ago Rinken, Toonika Rinken
Article
Chemistry, Analytical
Kairi Kivirand, Toonika Rinken
ANALYTICAL LETTERS
(2011)
Article
Biotechnology & Applied Microbiology
Margarita Kagan, Kairi Kivirand, Toonika Rinken
ENZYME AND MICROBIAL TECHNOLOGY
(2013)
Article
Multidisciplinary Sciences
Artur Gornischeff, Toonika Rinken
PROCEEDINGS OF THE ESTONIAN ACADEMY OF SCIENCES
(2011)
Article
Multidisciplinary Sciences
Delia Peedel, Toonika Rinken
PROCEEDINGS OF THE ESTONIAN ACADEMY OF SCIENCES
(2012)
Article
Chemistry, Analytical
Kairi Kivirand, Toonika Rinken
Article
Chemistry, Analytical
Kairi Kivirand, Riin Rebane, Toonika Rinken
Article
Environmental Sciences
E. Viirlaid, M. Ilisson, S. Kopanchuk, U. Maeorg, A. Rinken, T. Rinken
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2019)
Article
Biotechnology & Applied Microbiology
K. Mihklepp, K. Kivirand, D. Juronen, A. Lookene, T. Rinken
ENZYME AND MICROBIAL TECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Robin Ehrminger, Sergei Kopanchuk, Kairi Kivirand, Tavo Romann, Toonika Rinken, Mart Min, Ago Rinken
PROCEEDINGS OF THE ESTONIAN ACADEMY OF SCIENCES
(2020)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)