期刊
ANALYTICAL METHODS
卷 5, 期 1, 页码 187-191出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2ay25906k
关键词
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资金
- National Measurement Office
We present the first assay combining a dual aptamer sandwich format with detection by anodic stripping voltammetry with ionic silver amplification. This assay format lends itself to rapid point-of-care tests, where the use of aptamers could improve the overall stability of the assay. We have used human alpha-thrombin as a model system, and demonstrate a detection limit of 6.09 mu g L-1. We present the optimization of the aptamer-silver colloid attachment chemistry and the final assay format to achieve sensitive analyte detection. The use of a sandwich assay coupled with magnetic separation and ionic silver amplification, generates an assay with similar sensitivity than those reported in the literature in a format that can be used in rapid, portable testing regimes.
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