Article
Multidisciplinary Sciences
Aekkhaluck Intharuksa, Jessada Denduangboripant, Sunee Chansakaow, Kannika Thongkhao, Suchada Sukrong
Summary: This study identified twelve Mucuna species and examined aphrodisiac herbal products containing M. macrocarpa and M. pruriens using HPLC analysis and DNA barcoding. The results showed that L-dopa was present not only in the seeds of M. macrocarpa and M. pruriens, but also in associated allied Mucuna species. The integration of HPLC analysis and DNA barcoding profile was an efficient method for the identification of prohibited Mucuna species for safety monitoring of herbal supplements.
Article
Chemistry, Analytical
Pintao Li, Xiaowei Zeng, Huajie Xue, Xin Ye, Bin Yang, Jilie Kong, Liping Yin, Xueen Fang
Summary: DNA barcode identification has wide applications and great potential in various fields. The development of a CRISPR-microfluidic array allows for rapid and accurate identification of DNA barcodes, achieving consistent results with morphological identification in real-field seed detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Plant Sciences
Irene Bosmali, Georgios Lagiotis, Nadia Haider, Maslin Osathanunkul, Costas Biliaderis, Panagiotis Madesis
Summary: By analyzing DNA barcoding regions, this study identified 71 Vicia specimens of Eurasian descent. The trnL and ITS2 regions were effective in discriminating the analyzed species, while the rpoC1 region could not identify all selected species due to sequence conservation or missing sequences in GenBank. The combination of DNA barcoding regions is essential for accurate species discrimination within the Vicia genus.
Article
Plant Sciences
Mengying Chen, Jian Sun, Hui Yao, Fuyu Gong, Long Cai, Chanyan Wang, Qingsong Shao, Zhian Wang
Summary: This study examined the genetic and chemical variability among five Curcuma species using ITS2 and trnK intron gene fragment. The results showed that these gene fragments were able to distinguish the five species clearly. Additionally, there were significant differences in chemical composition, with C. longa showing the largest variation. These findings are important for selecting the original plants of Curcuma medicinal materials and discovering new medicinal plant resources.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Widad S. Al-Juhani, Kadry Ne Abdel Khalik
Summary: The study aimed to determine the phylogenetic relationships between Arabis alpina L. in Afro-Alpina Mountains of southwestern Saudi Arabia and its closely related species using DNA barcode, ITS2, matK and rbcL. The results indicated high success rates for DNA amplification and sequencing with ITS2 and matK, showing variations between A. alpina of Saudi Arabia and specimens from different geographical origins.
PAKISTAN JOURNAL OF BOTANY
(2021)
Article
Microbiology
Andrew W. Wilson, Ursula Eberhardt, Nhu Nguyen, Chance R. Noffsinger, Rachel A. Swenie, Justin L. Loucks, Brian A. Perry, Mariana Herrera, Todd W. Osmundson, Sarah DeLong-Duhon, Henry J. Beker, Gregory M. Mueller
Summary: The nrITS region is widely used in fungal diversity studies. Environmental metabarcoding has increased the importance of the fungal DNA barcode in documenting fungal diversity and distribution. The current understanding of the barcode gap in macrofungi is limited, hindering research on fungal diversity.
Article
Multidisciplinary Sciences
Mohammad Malek Faizal Azizi, Sohana Romeli, Hazana Razali, Eda Yuhana Ariffin, Muhammad Afiq Tajol Ariffin, Lee Yook Heng, Norliza Abu-Bakar, Han Yih Lau
Summary: A DNA biosensor utilizing electrochemical methods was developed for the rapid identification of the Musang King durian variety. The biosensor showed a low detection limit and a wide linear calibration range, making it a promising tool for field detection of the Musang King variety.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Wenqiang Chen, Xiaoyun Chen, Junfeng Xu, Jian Cai, Xiaofu Wang
Summary: In this study, DNA barcoding and SNP-specific sites were used to identify Dendrobium officinale and differentiate it from other Dendrobium species. The results showed that the ITS2 barcode had the strongest identification capability. This study provides a new perspective on the accurate identification of D. officinale samples and the assessment of medicinal material quality for species with similar evolutionary histories.
FOOD ANALYTICAL METHODS
(2022)
Article
Plant Sciences
Wen-Pan Dong, Jia-Hui Sun, Yan-Lei Liu, Chao Xu, Yi-Heng Wang, Zhi-Li Suo, Shi-Liang Zhou, Zhi-Xiang Zhang, Jun Wen
Summary: The study used phylogenomic data to resolve relationships within the genus Olea and identify molecular markers for species identification. The results showed that the genus Olea is not monophyletic and provided insights into DNA barcodes for accurate species identification.
JOURNAL OF SYSTEMATICS AND EVOLUTION
(2022)
Article
Plant Sciences
Kaihui Zhao, Lianqiang Li, Hong Quan, Junbo Yang, Zhirong Zhang, Zhihua Liao, Xiaozhong Lan
Summary: This study sequenced and analyzed six Zanthoxylum chloroplast genomes, identifying specific genomic characteristics and mutational hotspots, with seven DNA barcode sequences for species differentiation. The phylogenetic analysis supported the existence of the Fagara subgenus within Zanthoxylum and proposed the potential for a new subgenus. The availability of these genomes provides valuable information for species identification, molecular breeding, and evolutionary analysis of Zanthoxylum.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Julia Karla A. M. Xavier, Leonardo Maia, Pablo Luis B. Figueiredo, Adriana Folador, Alessandra R. Ramos, Eloisa H. Andrade, Jose Guilherme S. Maia, William N. Setzer, Joyce Kelly R. da Silva
Summary: The study focused on Lauraceae species Aniba in the Amazon region, aiming to discriminate between species based on chemical and molecular markers. Through statistical multivariate and phylogenetic analysis, a significant correlation was found between volatile chemical classes and genetic characteristics of the Aniba species.
Article
Multidisciplinary Sciences
Widad Saleem Al-Juhani, Kadry Ne Abdel Khalik
Summary: This study focused on six commonly found Plectranthus species in Saudi Arabia and evaluated the efficiency of five different plastid DNA barcodes in identifying and distinguishing between these species. The results show that the nuclear ITS2 region can effectively discriminate between species and has a clear barcode gap.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Plant Sciences
Auni Aqilah Ahmad Tarmizi, Alina Wagiran, Faezah Mohd Salleh, Lee Suan Chua, Farah Izana Abdullah, Rosnani Hasham, Suraiya Binte Mostafiz
Summary: The study focuses on authenticity testing using a combination of DNA barcoding and HPLC, successfully generating DNA reference barcodes for Labisia pumila and using them to identify species in herbal products, while also using HPLC to assess their quality. The findings suggest the importance of both methods in providing strong evidence for species identification and authenticity examination of herbal products.
Review
Biochemistry & Molecular Biology
Ankush D. Sawarkar, Deepti D. Shrimankar, Manish Kumar, Phani Kumar, Sunil Kumar, Lal Singh
Summary: Bamboo, with its diverse species, benefits from the utilization of DNA barcoding technology for classification and identification, overcoming challenges of traditional methods. Different barcode regions such as matK, rbcL, and rpoB are used to distinguish bamboo species from different genera.
MOLECULAR BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yong Kang, Peiwei Liu, Feifei Lv, Yuxiu Zhang, Yun Yang, Jianhe Wei
Summary: This study accurately identified the source species of Qi-Nan germplasm as A. sinensis through DNA barcoding analysis, with ITS2 and matK showing the best performance in species identification.