期刊
ANALYTICAL METHODS
卷 3, 期 8, 页码 1859-1864出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ay05108c
关键词
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资金
- China Postdoctoral Science Foundation [20100471297]
- Jiangsu Planned Projects for Postdoctoral Research Funds [0902065C]
- National High-Tech Research and Development Program of China [2006AA06Z411]
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed and successfully applied in a dissolved mercury assay. For mercury quantification, factors including the concentration of chelator EDTA, pH, ionic strength, and blocking solution were optimized in this study. Generally the detection limit of the ELISA was standardized as 0.459 mu g L(-1), with an IC(50) value of 13.09 mu g L(-1) and linear range of 1 to 500 mu g L(-1); whereas a cross-reactivity study showed that the immunoassay was quite specific for mercury, compared with nine other metal ions. The recovery of the method was evaluated by spiking ultrapure water, tap water and surface water of Taihu Lake with mercury at 1, 10, 50, 100 mu g L(-1). The precision of the method was evaluated by spiking ultrapure water with mercury at 1, 10, 50, and 100, 500 and 1000 mu g L(-1). The coefficients of variation of intra-and inter-assay were below 10% in most cases, and recoveries were 94.3-123.5%, 91.9-117.9%, and 92.3-115.6% for ultrapure water, tap water and surface water of Taihu Lake, respectively. Therefore, the developed method is able to determine mercury at levels below the maximum residue limits of water quality standards for drinking water sources of China.
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