Article
Chemistry, Analytical
Xiaona Li, Zhini Liu, Zhuo Li, Xin Xiong, Xianhua Zhang, Changqing Yang, Libo Zhao, Rongsheng Zhao
Summary: A novel LC-MS method was developed for simultaneous quantification of 16 purine metabolites in human plasma and urine. The method showed good linearity, low quantitation limits, and stable recovery and matrix effects. The method was successfully applied to compare the plasma and urine samples of patients without and with acute kidney injury (AKI), revealing potential upregulation of certain purine intermediates in AKI patients, indicating an association with inflammatory responses.
Article
Chemistry, Analytical
Yuxiu Li, Zengyang He, Peng Zou, Yong Ning, Xiaolan Zhu
Summary: In this study, a rapid and sensitive HILIC-ESI-MS/MS method combining UAE and SPE purification was developed to accurately determine the content and distribution of sugars and sugar alcohols in fruit juice samples. The method involved ultrasonic-assisted extraction and solid phase extraction to extract and purify the compounds. The validated HILIC-MS/MS method showed good performance in terms of quantification and recovery, and was successfully applied to profile the sugars and sugar alcohols in various fruit juice matrices.
MICROCHEMICAL JOURNAL
(2023)
Article
Medical Laboratory Technology
Lavinia Santucci, Sara Lomuscio, Aniello Primiano, Riccardo Calvani, Silvia Persichilli, Federica Iavarone, Anna Picca, Francesca Canu, Andrea Urbani, Jacopo Gervasoni
Summary: This study aimed to develop a rapid method for the simultaneous determination of arginine and its metabolites in human plasma. A simple deproteinization procedure and hydrophilic interaction liquid chromatography were used for the pre-analytical and chromatographic separation, respectively. The method showed good recovery, precision, and stability, making it suitable for both research and clinical routine use.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Dmitri Pismennoi, Vassili Kiritsenko, Jaroslav Marhivka, Mary-Liis Kutt, Raivo Vilu
Summary: A method for quantifying five saccharides in fermentation samples produced by Streptococcus thermophilus was developed, allowing for accurate and high-throughput analysis.
Article
Biochemical Research Methods
Xiaoxiao Zhang, Yiqiu Hu, Xiujuan Hong, Mingwei Wang, Zhihao Fang, Xiaoji Cao, Kezhi Jiang, Cheng Guo
Summary: RNA modifications play essential roles in biological activities and are associated with human diseases, including cancers. This study established a method to quantify six nucleosides in urine and plasma samples, and found altered concentrations of certain nucleosides in breast cancer patients. Furthermore, the ratios of methylated adenosine nucleosides to adenosine in plasma were better at distinguishing breast cancer patients from healthy controls.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Biochemical Research Methods
Pauline Bosman, Valerie Pichon, Ana Carolina Acevedo, Helene Chardin, Audrey Combes
Summary: Advancements in metabolomics have allowed for the identification and characterization of saliva metabolites as biomarkers, but discrepancies in biomarker content for a given disease have been observed. Differences in saliva collection, storage, and treatment methods could contribute to these discrepancies, as there is currently no standardized method. This study investigated the effects of different collection methods on saliva metabolite analysis, and found that saliva obtained through spitting and aspiration yielded comparable results, while the Salivette (R) method did not appear suitable for untargeted analysis of the salivary metabolome.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
E. Boutou, C. Virgiliou, A. K. Seliniotaki, M. Lithoxopoulou, A. Mataftsi, N. Ziakas, E. Diamanti, N. Raikos, H. Gika
Summary: A sensitive and accurate HILIC-MS/MS method was developed to determine the concentration of phenylephrine in DBS samples from preterm infants after ocular administration. The method involved extracting the analyte using methanol - acetonitrile and separating it on an ACQUITY UPLC BEH AMIDE column. Detection was achieved using electrospray ionization.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Environmental Sciences
Lei Pan, Jinhui Jeanne Huang, Junhui Chen, Xiuping He, Yuning Wang, Jiuming Wang, Baodong Wang
Summary: This study developed a new method to detect hydrophilic cyanotoxins in freshwater and discovered the presence of various toxins in freshwater in China. The proposed method helps understand the pollution status of different cyanotoxins in water bodies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Chang Shu, Teng-Fei Li, Duo Li, Zhong-Qiu Li, Xing-Hua Xia
Summary: Pomalidomide, an IMiD approved by the FDA for multiple myeloma treatment, was quantified in human plasma using a validated LC-MS/MS method. The method was successfully applied to clinical pharmacokinetic studies with positive results.
CHINESE CHEMICAL LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Zhihao Fang, Yiqiu Hu, Xiujuan Hong, Xiaoxiao Zhang, Tao Pan, Chi Pan, Shu Zheng, Cheng Guo
Summary: RNA methylation plays a vital role in diseases like cancer. Researchers have developed a sensitive method to accurately quantify methylated nucleosides in human urine, revealing an association between the contents of urinary methylated nucleosides and the occurrence of breast cancer.
Article
Biochemical Research Methods
Yao Liu, Zhichao He, Heng Liang, Minzhen Han, Jinxingyi Wang, Qian Liu, Yanping Guan
Summary: A high-throughput UHPLC-MS/MS bioassay has been developed for simultaneous quantification of multiple anti-tumor drugs. The validated approach is reliable and effective in clinical management, providing valuable support in therapeutic drug monitoring and individualized dosing optimization.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Analytical
Fumin Li, Gang Xing, Christopher Cousineau, Sara Clemens, Melissa Mofikoya, Moo-Young Kim, Jenny (Yanhua) Zhang, Yizhong Zhang, Nancy Raha
Summary: In this study, a LC-MS/MS method for the quantitation of fructose in human urine was developed and validated. Difficulties were encountered for the post-extraction stability experiments, particularly for authentic fructose QCs at low concentrations. However, a column cleaning procedure was implemented to overcome this issue. The validated HILIC-MS/MS method was successfully applied to analyze clinical samples with a 91% overall run passing rate.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Dimitrios J. Floros, Kangkang Xu, Franz Berthiller, Heidi Schwartz-Zimmermann
Summary: Metabolomics is gaining popularity in livestock research, and this study compared different chromatographic methods to assess their coverage of mammalian metabolites. The study investigated 354 primary metabolites from various compound classes and optimized selected reaction monitoring transitions on a mass spectrometer. The results showed that hydrophilic interaction liquid chromatography (HILIC) methods had the highest coverage of metabolites, including polar ones such as amino acids and biogenic amines. Combining complementary HILIC and reversed-phase (RP) methods increased the coverage to 92%, and further incorporating anion exchange chromatography (AIC) achieved a coverage of 97%.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Shi-Yu Wen, Hong Ma, Xiang-Long Chen, Yang Zhao, Yan Liu, Yue Li, Chao Lu, Yong-Qing Wang, Lu-Ning Sun
Summary: A HILIC-MS/MS method was developed and validated to determine the levels of caffeine and its major metabolites in the plasma of premature infants. This method showed good linearity and accuracy within the specified concentration ranges.
Article
Chemistry, Analytical
Lana Abou-Zeid, Albert Pell, Marta Garcia Cortes, Helene Isnard, Pascale Delangle, Carole Bresson
Summary: This study developed an analytical method to determine the affinity of biomimetic peptides for uranyl ions and evaluate the effect of structural parameters on this affinity. By combining hydrophilic interaction chromatography (HILIC) with electrospray ionization mass spectrometry (ESI-MS) and inductively coupled plasma mass spectrometry (ICP-MS), the UO22+ complexes formed with different peptides were successfully separated and quantified. The results showed that an increasing number of phosphorylated residues promotes the affinity of the peptides for UO22+, while the position of the phosphorylated residues in the peptide backbone has minimal impact on this affinity, and the cyclic structure of the peptide favors the complexation with UO22+ compared to the linear structure.
ANALYTICA CHIMICA ACTA
(2023)
Article
Substance Abuse
Corey J. Hayes, Erin E. Krebs, Chenghui Li, Joshua Brown, Teresa Hudson, Bradley C. Martin
Summary: This study found that discontinuing chronic opioid therapy is associated with decreased diagnoses of opioid-related adverse outcomes, but the association with substance use disorders remains inconclusive.
Article
Nutrition & Dietetics
Sarah K. Kirschner, Nicolaas E. P. Deutz, Iris Rijnaarts, Tiffany J. Smit, Daniel J. Larsen, Marielle P. K. J. Engelen
Summary: The study found impaired protein digestion and absorption, altered plasma SCFA concentrations in CHF patients, which are associated with reduced muscle and cognitive health as well as overall well-being. More research is needed to implement strategies to improve intestinal function in CHF and to explore the mechanisms underlying its connection with other systemic manifestations.
JOURNAL OF PARENTERAL AND ENTERAL NUTRITION
(2022)
Article
Medicine, General & Internal
Golnoosh Alipour-Haris, Melissa J. Armstrong, Jennifer L. Sullivan, Uma Suryadevara, Masoud Rouhizadeh, Joshua D. Brown
Summary: This study aimed to investigate the burden of suicidal behaviors among patients with AD and ADRDs, and found differences in the frequency of suicidal-behavior-related hospitalizations across dementia subtypes.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Motomori O. Lewis, Phuong T. Tran, Yushi Huang, Raj A. Desai, Yun Shen, Joshua D. Brown
Summary: Pneumonia is a major cause of hospitalization in pediatric patients, and its severity is associated with readmission rates. Severe pneumonia has higher readmission rates and higher hospital costs. Factors associated with readmission include pneumonia severity, immunosuppressive conditions, length of stay, and hospital case volume.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Brian Cicali, Stephan Schmidt, Markus Zeitlinger, Joshua D. Brown
Summary: This study utilized pharmacoepidemiology methods and pharmacology principles to estimate the risk of macrolide treatment failure and quantify the influence of their pharmacokinetics on the risk of treatment failure. The results showed that macrolide AUC changes greater than 50% had a significant effect on treatment failure risk.
Article
Clinical Neurology
Brianna Costales, Scott M. Vouri, Joshua D. Brown, Barry Setlow, Amie J. Goodin
Summary: This study examines the pharmacotherapy treatment patterns for early-onset idiopathic RLS and finds that ropinirole, pramipexole, and gabapentin are the most commonly initiated treatments. However, persistence is low and the use of FDA-approved drugs for RLS is less frequent.
Article
Computer Science, Information Systems
Anurag Das, Bobak Mortazavi, Seyedhooman Sajjadi, Theodora Chaspari, Laura E. Ruebush, Nicolaas E. Deutz, Gerard L. Cote, Ricardo Gutierrez-Osuna
Summary: This study examines the impact of additional dietary biomarkers in blood on macronutrient prediction and finds that they improve prediction performance compared to using continuous glucose monitors alone. The findings suggest a potential solution to the problem of diet monitoring and may facilitate the development of automated methods for monitoring nutritional intake.
IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS
(2022)
Article
Nutrition & Dietetics
Raven A. Wierzchowska-McNew, Marielle P. K. J. Engelen, John J. Thaden, Gabriella A. M. ten Have, Nicolaas E. P. Deutz
Summary: This study aimed to investigate the association between obesity, sex, and sex-by-obesity interaction on whole-body arginine kinetics. The findings suggest that alterations in arginine metabolism are present in morbid obesity, but further research is needed to explore the factors related to these changes.
AMERICAN JOURNAL OF CLINICAL NUTRITION
(2022)
Article
Nutrition & Dietetics
Katherine L. Ford, Claire F. Trottier, Wendy V. Wismer, Michael B. Sawyer, Mario Siervo, Nicolaas E. P. Deutz, Carla M. Prado, Helen Vallianatos
Summary: This qualitative study explored the dietary decisions made by patients with colorectal cancer (CRC) starting chemotherapy. The results showed that patients' dietary choices were influenced by medical factors, health beliefs, fixed diets, and external influences. Understanding these factors can guide healthcare providers in providing tailored dietary support for CRC patients.
JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS
(2023)
Article
Geriatrics & Gerontology
Wayne W. Campbell, Nicolaas E. P. Deutz, Elena Volpi, Caroline M. Apovian
Summary: This narrative review discusses the impact of dietary protein intake on muscle-related attributes of older adults. Insufficient protein intake exacerbates age-related reductions in muscle size, quality, and function. Adequate protein intake promotes muscle size and function, with observational studies suggesting higher protein intake may enhance muscle strength and function. Higher protein intake benefits lean body mass changes under stress conditions. Specialized protein supplements can help attenuate muscle loss in older adults with medical conditions or acute illness. Animal protein sources are preferred for sarcopenia-related parameters. The quantity, quality, and patterning of dietary protein intake are important for supporting muscle size and function in older adults.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Editorial Material
Nutrition & Dietetics
Nathalie Delzenne, Nicolaas E. Deutz, Dileep N. Lobo, George Grimble
Summary: In this education paper, advice is given on successful scientific grant writing. The paper emphasizes the importance of defining research hypothesis, fulfilling technical aspects, and understanding the requirements before writing the proposal. It also highlights the need to develop a timeline, create an attractive summary, and set SMART research objectives. Additionally, the paper emphasizes the importance of making the proposal understandable to non-experts while providing sufficient details for experts.
CLINICAL NUTRITION ESPEN
(2023)
Article
Nutrition & Dietetics
Nicolaas E. Deutz, Nathalie Delzenne, George Grimble, Dileep N. Lobo
Summary: Publishing a scientific paper is a challenging process that depends on the quality of study design, research questions, clear primary endpoints, and data from a large population. Before submission, it is important to meet ethical requirements, register in public databases, and declare any conflicts of interest. The structure of the manuscript includes sections for introduction, materials and methods, results, discussion, and an abstract that summarizes all aspects concisely. The publication process involves editors, reviewers, and publishers, with the initial impression being crucial for review. Thoughtful responses to reviewers' feedback are necessary for revisions. The goal is acceptance and publication of the paper.
CLINICAL NUTRITION ESPEN
(2023)
Article
Nutrition & Dietetics
George Grimble, Dileep N. Lobo, Nathalie Delzenne, Nicolaas E. Deutz
Summary: When presenting a paper, it is important to tailor your presentation to the audience's knowledge level and the title and abstract you submitted. Keep the talk simple and avoid complexity. Stick to the scheduled time and present the information in a concise manner. Make sure your slides are clear and informative, readable from a distance. Practice the talk beforehand, preferably with colleagues, and be prepared to make any necessary changes. Familiarize yourself with the content so you can relax and enjoy the experience.
CLINICAL NUTRITION ESPEN
(2023)
Article
Nutrition & Dietetics
Dileep N. Lobo, George Grimble, Nathalie Delzenne, Nicolaas E. Deutz
Summary: There are potential pitfalls in the publication process that researchers may encounter, whether knowingly or unknowingly. Disregarding publication governance can be risky and harm the reputation of authors and co-authors, even if the errors are unintentional. This article highlights these pitfalls, introduces relevant rules in research and publishing, and guides readers on how to avoid stumbling on the path to publication.
CLINICAL NUTRITION ESPEN
(2023)
Review
Nutrition & Dietetics
Nina Kaegi-Braun, Fiona Kilchoer, Saranda Dragusha, Carla Gressies, Montserrat Faessli, Filomena Gomes, Nicolaas E. Deutz, Zeno Stanga, Beat Mueller, Philipp Schuetz
Summary: This systematic review suggests that postdischarge nutritional support in the outpatient setting can lower all-cause mortality, increase nutritional intake, and improve body weight in malnourished patients.
CLINICAL NUTRITION
(2022)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)