4.4 Article

Immobilization of Glucose Oxidase and 2-Hydroxybiphenyl 3-Monooxygenase in Mesoporous Silica: Characterization Studies and Construction of an Amperometric Glucose Biosensor

期刊

ANALYTICAL LETTERS
卷 43, 期 16, 页码 2582-2597

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/00032711003725664

关键词

2-Hydroxybiphenyl 3-monooxygenase (HpbA); Glucose oxidase (GOx); Immobilization; Silica MCM-41

资金

  1. EU [QLRT-2001-01629]
  2. Greek Ministry of Development-General Secretariat of Research and Technology

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Immobilization techniques that preserve the activity of biomolecules have many potential applications. Mesoporous silica has been used to encapsulate a wide variety of biomolecules. In this particular work, MCM-41 silica has been used as a matrix for the immobilization of two important enzymes, HpbA and GOx. The enzyme 2-hydroxybiphenyl 3-monooxygenase (HpbA) is involved in the microbial degradation of 2-hydroxy and 2,2'-dihydroxybiphenyl, which occurs during the desulfurization of coal and petroleum, while Glucose oxidase (GOx) catalyzes the oxidation of -D-glucose to -gluconolactone. The silica-enzyme hybrids were extensively characterized and the one with GOx was used for the construction of a biosensor.

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