Article
Microbiology
Guangpu Yang, Bingqian Zhou, Kewei Chen, Zhe Hu, Wei Guo, Xiaojun Wang, Cheng Du
Summary: Novel competitive ELISA and western blot assays were developed to detect antibodies against T. equi or B. caballi. These methods have high sensitivity, no cross-reactivity with other equine diseases, and showed high coincidence rates compared to commercially available kits.
Article
Biotechnology & Applied Microbiology
Christina Seisenberger, Tobias Graf, Markus Haindl, Harald Wegele, Michael Wiedmann, Stefanie Wohlrab
Summary: Through a study comparing ELISAs and 2D-WBs for HCP monitoring, it was found that ELISAs are better suited for accurate detection of HCPs, while 2D-WBs have some limitations.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Fisheries
Evan M. Jones, Luke P. Oliver, Jie Ma, Robine H. J. Leeuwis, Veronica Myrsell, Mary R. Arkoosh, Joseph P. Dietrich, Cameron M. Schuster, Matt Hawkyard, A. Kurt Gamperl, Kenneth D. Cain
Summary: A monoclonal antibody specific to sablefish IgM was produced and demonstrated its usefulness in studying antibody-mediated immunity, vaccine development, and aquaculture efforts for this fish species. The antibody, UI-25A, can recognize membrane-bound IgM and identify immunogenic regions of A. salmonicida.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Michaela Jakubikova, Jana Sadecka, Katarina Hrobonova
Summary: The aim of this study was to develop a simple, fast, and reagent-free method for predicting the total phenolic content and selected phenolic compounds in Slovak botrytized Tokaj wines using spectral data and partial least squares regression. The best predictive model achieved an RPD of 5.8, R2 of 0.972, and RMSE of 20.2 mg/L for TPC. The PLS calibration models for gallic, protocatechic, caffeic, p-coumaric acids, and (+)-catechin had high prediction accuracy with RPD > 4.0 and R2 > 0.9.
MICROCHEMICAL JOURNAL
(2022)
Article
Parasitology
Eya Ben Salah, Coralie Barrera, Sana Mosbahi, Bruno Gottstein, Mar Siles-Lucas, Samia Belhassen, Abdellatif Nouri, Hamouda Babba, Laurence Millon, Wahiba Sakly
Summary: The study investigated the value of antigenic proteins from Echinococcus granulosus protoscoleces and recombinant B2t and 2B2t proteins in assessing the efficacy of surgical treatment for CE-affected children. Results showed that B2t protein could be useful for early post-surgical outcome prediction, while protoscolex proteins like P26/27 and P40 may serve as promising predictive biomarkers for post-surgical follow-up of CE cases.
PARASITES & VECTORS
(2021)
Article
Environmental Sciences
Sijia Hua, Jiawen Chen, Liang Wu, Xinyue Yu, Jing Ye, Yuanting Li, Yongqiang Zhu, Fuxiang Tian
Summary: This study detected the contents of MC-LR in Microcystis aeruginosa cultures and water samples from the Huangpu River using ELISA and HPLC methods. The results showed that ELISA was a reliable and simple method for monitoring low levels of MC-LR. The concentration of MC-LR in the Huangpu River varied with the season, but remained below the WHO drinking water quality standard.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemical Research Methods
Qiana Mendez, Holland A. Driscoll, Gregory R. Mirando, Felicity Acca, Cassandra D. Chapados, Kezzia S. Jones, Michael Weiner, Xiaofeng Li, Mary R. Ferguson
Summary: Knowing the accuracy of an antibody's sensitivity and specificity is crucial for reliable data collection. Two validation methods, MILKSHAKE and Sundae, are described for antibodies binding post-translationally modified proteins. These methods improve evaluation of commercially available antibodies and identify off-target effects for research and therapeutic purposes.
JOURNAL OF IMMUNOLOGICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Muhammad Faheem, Arif-ullah Khan, Muhammad Waqas Saleem, Fawad Ali Shah, Fawad Ali, Abdul Waheed Khan, Shupeng Li
Summary: This study explored the effects of natural compounds, berbamine, bergapten, and carveol, on neuroinflammatory pain associated with paclitaxel. The results showed that these compounds attenuated thermal hypersensitivity, improved motor function, normalized body weight changes, and increased the pain threshold. They also demonstrated neuroprotective effects by regulating molecular pathways.
Article
Chemistry, Applied
Agnieszka Mierczynska-Vasilev, Keren Bindon, Richard Gawel, Paul Smith, Krasimir Vasilev, Hans-Jurgen (Hans-Juergen) Butt, Kaloian Koynov
Summary: The study used FCS to investigate the interaction between wine macromolecules and BSA, showing changes in protein conformation and complex formation with the addition of wine macromolecules. Different fractions in wine had varied effects on aggregation, with polysaccharides preventing protein-tannin complex precipitation. The application of FCS in real wines demonstrated live monitoring of heat-induced aggregation to study protein haze formation.
Article
Microbiology
Sarfraz-ur-Rahman, Haroon Akbar, Muhammad Zubair Shabbir, Ubaid Ullah, Muhammad Imran Rashid
Summary: The study evaluated the diagnostic value of the recombinant SAG1 antigen for T. gondii-IgG screening. The ELISA method showed high sensitivity and specificity, but may produce false positive results in some cases.
Article
Parasitology
Marie-Kristin Raulf, Daniela Jordan, Herbert Auer, Jens M. Warnecke, Bernd Lepenies, Christina Strube
Summary: This study evaluated the newly developed Anti-Toxocara-ELISA and Anti-Toxocara-Westernblot prototypes, which showed high sensitivity and specificity, indicating high reliability of these newly developed tests.
Article
Food Science & Technology
Wen-Juan Song, Pei-Pei Liu, Chun-Bao Li, Hui-Xia Li, Shi-Jie Ding, Guang-Hong Zhou
Summary: In this research, a method of high performance liquid chromatography (HPLC) was established to quantify the potential residual content of dexamethasone, indomethacin, isobutylmethylxanthine, and rosiglitazone in cultured fat. The analysis showed that the content of these residues decreased to zero by Day 10. Additionally, the insulin content in the cultured fat was measured using enzyme-linked immunosorbent assay (ELISA) and it was found to be 2.78+/- 0.21 mu g/kg on Day 10, which decreased to 1.88 +/- 0.54 mu g/kg after soaking with phosphate buffered saline (PBS). In conclusion, this research provides an effective approach to determine the potential residual components in cultured fat and will contribute to the future safety assessment of cultured fat.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Jana Sadecka, Michaela Jakubikova, Katarina Hrobonova, Lubomir Svorc, Marek Hasso
Summary: Non-targeted UV-Vis/SF spectroscopic and DPV fingerprinting were used to discriminate between caramel-containing and caramel-free wine spirits. High correct classification rates were achieved using VIP-PLS-DA and VIP-PCA-LDA analysis. The conclusions were verified by targeted HPLC-DAD analysis.
MICROCHEMICAL JOURNAL
(2023)
Article
Immunology
Jawad Khan, Gowhar Ali, Asma Khurshid, Aamer Saeed, Sajjad Ahmad, Najeeb Ullah, Ashrafullah Khan, Robert D. Sewell, Muhammad Zakria
Summary: The study demonstrated that novel compounds KB9 and KB10 alleviated vincristine induced neuropathy through anti-inflammatory, anti-nociceptive and antioxidant mechanisms.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Veterinary Sciences
Martha Maggira, Ioannis Sakaridis, Maria Ioannidou, Georgios Samouris
Summary: This study compared three available ELISA kits for the detection of aflatoxins in feed samples. All three kits showed good performance and were validated. Furthermore, the results of both ELISA and HPLC tests confirmed their suitability for mycotoxin analysis.
VETERINARY SCIENCES
(2022)
Article
Electrochemistry
Camelia Albu, Sandra A. V. Eremia, Monica Lucia Veca, Andrei Avram, Radu Cristian Popa, Cristina Pachiu, Cosmin Romanitan, Mihaela Kusko, Raluca Gavrila, Antonio Radoi
ELECTROCHIMICA ACTA
(2019)
Article
Biochemistry & Molecular Biology
Adina Boldeiu, Monica Simion, Iuliana Mihalache, Antonio Radoi, Melania Banu, Pericle Varasteanu, Paul Nadejde, Eugeniu Vasile, Adriana Acasandrei, Roxana Cristina Popescu, Diana Savu, Mihaela Kusko
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Cosmin Romanitan, Mihaela Kusko, Marian Popescu, Pericle Varasteanu, Antonio Radoi, Cristina Pachiu
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2019)
Article
Chemistry, Multidisciplinary
Pericle Varasteanu, Cosmin Romanitan, Alexandru Bujor, Oana Tutunaru, Gabriel Craciun, Iuliana Mihalache, Antonio Radoi, Mihaela Kusko
Article
Chemistry, Applied
Marius C. Stoian, Iuliana Mihalache, Mihaela Matache, Antonio Radoi
Summary: A new luminescent material based on silica nanoparticles functionalized with a terbium complex exhibited fluorescence in aqueous and organic solvents. The material was effective in sensing metal ions, particularly showing strong quenching effects for iron(III), copper(II), and cobalt(II) ions.
Article
Chemistry, Physical
Irina-Nicoleta Bratosin, Pericle Varasteanu, Cosmin Romanitan, Alexandru Bujor, Oana Tutunaru, Antonio Radoi, Mihaela Kusko
Summary: The study focuses on increasing the performance of supercapacitors through porosification of silicon electrodes to increase active surface area and coating electrodes for higher capacitance and better performance.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Materials Science, Multidisciplinary
Madalina Tudose, Rodica D. Baratoiu-Carpen, Elena Maria Anghel, Mariana Voicescu, Simona Somacescu, Daniela C. Culita, Anamaria Hanganu, Andrei Kuncser, Antonio Radoi
Summary: A new composite material was successfully synthesized by covalently grafting pyrene thiazole onto the surface of graphene oxide. Various characterization methods confirmed the successful functionalization of graphene oxide with pyrene thiazole, showing potential for detecting caffeic acid.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Review
Chemistry, Analytical
Reem Khan, Antonio Radoi, Sidra Rashid, Akhtar Hayat, Alina Vasilescu, Silvana Andreescu
Summary: Advancements in functional nanomaterials and precisely designed nanostructures have created new opportunities for practical biosensors with enhanced performance and reliability for detecting various analytes. By combining different types of nanostructures with bio-elements, higher selectivity and sensitivity can be achieved. Overcoming manufacturing challenges and further exploring the application of these nanomaterials in biosensors will be crucial for future developments.
Article
Materials Science, Multidisciplinary
Pericle Varasteanu, Cosmin Romanitan, Irina Bratosin, Nikolay Djourelov, Raluca Gavrila, Antonio Radoi, Mihaela Kusko
Summary: The study constructed a symmetric micro-supercapacitor by co-depositing molybdenum disulfide nanoflakes and poly-naphthalenemethylamine on micro-patterned palladium current collectors over a plastic substrate. The polymer/MoS2 nanocomposite provided the framework necessary for charge transport and ion intercalation, resulting in high volumetric capacitance. By using a PVA-H2SO4 solid gel electrolyte, a 3.1 V potential window was achieved, showing potential for further improvement of the MSC's performance.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Pericle Varasteanu, Antonio Radoi, Oana Tutunaru, Anton Ficai, Razvan Pascu, Mihaela Kusko, Iuliana Mihalache
Summary: The study focuses on developing self-powered photodetectors with silicon nanoholes (SiNHs) and four different metal nanowires (AgNWs, AuNWs, NiNWs, PtNWs), showing the AgNWs/SiNHs device performs the best in the blue light region and the AuNWs/SiNHs device has significantly enhanced sensitivity in the red and near-infrared spectral region.
Article
Electrochemistry
Irina-Nicoleta Bratosin, Cosmin Romanitan, Gabriel Craciun, Nikolay Djourelov, Mihaela Kusko, Marius C. Stoian, Antonio Radoi
Summary: In this work, a scalable route to wafer-size processing for fabrication of hybrid nanocomposite electrodes based on cobalt hexacyanoferrate/carbon/porous silicon (CoHCF/C/Si) is reported. The fabricated symmetric supercapacitor device exhibits excellent volumetric capacitance, power density, and energy density, as well as good cycling stability. The successful integration of CoHCF nanocubes with carbonaceous and silicon material using electrochemical methods results in synergistic effects over the electrochemical performances.
ELECTROCHIMICA ACTA
(2022)
Article
Chemistry, Physical
Larisa Gogianu, Marian C. Popescu, Bogdan S. Vasile, Iuliana Mihalache, Elena M. Anghel, Celina M. Damian, Alexandru Salceanu, Adina Boldeiu, Elena Constantin, Antonio Radoi, Monica Simion, Melania A. Popescu
Summary: Recent studies have shown that human oncoviruses account for 10% of cancer cases worldwide. PCR assays are highly sensitive and specific, while microarrays offer excellent performance for detecting multiple targets simultaneously. However, accurate detection of DNA viruses in microarray systems depends on the platform used, and obtaining high-quality fluorescent-labelled single-stranded DNA is equally important.
APPLIED SURFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
A. Cristina Costache, Gabriel Moagar-Poladian, Cosmin A. Obreja, Oana Tutunaru, Antonio Radoi, Cristina Pachiu
Summary: This study presents the experimental results on the recycling process of waste PA2200 powder from Selective Laser Sintering, aiming to show a decontamination route for recovery and further recycling of the waste to achieve a cleaner and more eco-friendly SLS process. The decontamination process and detailed characterization of the recovered waste are also described using SEM EDX, FTIR, and Raman spectroscopy.
UNIVERSITY POLITEHNICA OF BUCHAREST SCIENTIFIC BULLETIN SERIES B-CHEMISTRY AND MATERIALS SCIENCE
(2021)
Article
Electrochemistry
Octavian-Gabriel Simionescu, Cosmin Romanitan, Camelia Albu, Cristina Pachiu, Eugeniu Vasile, Nikolay Djourelov, Oana Tutunaru, Marius Constantin Stoian, Mihaela Kusko, Antonio Radoi
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2020)
Article
Chemistry, Multidisciplinary
Codruta C. Popescu, Marius C. Stoian, Lia-Maria Cucos, Anca G. Coman, Antonio Radoi, Anca Paun, Niculina Hadade, Arnaud Gautier, Costin-Ioan Popescu, Mihaela Matache
